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Journal of the Egyptian Society of Parasitology. 1993; 23 (1): 109-132
en Inglés | IMEMR | ID: emr-28353

RESUMEN

Specificity and sensitivity are very important when investigating the blood meals of an insect vector, particularly those having different hosts. Several techniques are used for the identification of the blood meals. Counter immunoelectrophoresis is the technique of choice. The modification was to secure saving of time and least amount of materials. In this paper, a modified technique of the counter immunoelectrophoresis [CIEP] was used for the identification of the blood meal of wild Phlebotomus papatasi collected from different sites in Qalyoubia Governorate. Eight antisera were used. These were human, cat, dog, rat, sheep, goat, bovine, and avian. The results showed that 99.52% of 832 female P. papatasi contained human blood, 0.12% [one female] contained only avian blood, and 0.36% or three females each contained mixed blood of human together with dog blood [one], rat blood [one] or avian blood [one]. P. papatasi is an anthropophilic insect, but in rare cases, it is zoophilic


Asunto(s)
Contrainmunoelectroforesis/métodos , Phlebovirus/aislamiento & purificación
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