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ObjectiveTo investigate the Alzheimer-associated neurofilament protein （AD7c-NTP） in urine of middle-aged and elderly people and its correlation between common metabolites. MethodsA total of 1 150 middle-aged and elderly people who did their physical exmanination in the health examination center of the Sichuan Science City Hospital and the Third Hopital of Mianyang were recruited from March 2017 to March 2020. The level of urine AD7c-NTP were measured by enzyme-linked immunosorbent assay （ELISA）， and common metabolites in blood were measured by biochemical analyzer. Based on urine AD7c-NTP level ≤1.5 ng/mL， the objects was divided into normal group （n=956） and elevated group （n=194）. Thier demographic data and blood biochemical indicators were collected. ResultsThe urine AD7c-NTP level in middle-aged and elderly people was 0.60（0.30~1.20） ng/mL. The urine AD7c-NTP level was higher in women than that in men ［1.04（0.40~1.30） ng/mL vs. 0.84（0.30~1.00） ng/mL， Z=4.202， P˂0.01］. And the urine AD7c-NTP level was lower in the normal group than that in the elevated group ［0.50（0.30~0.90） ng/mL vs. 2.10（1.70~2.10） ng/mL， Z=22.035， P˂0.01］. The results of the univariate comparison showed that， the differences between the two groups in age （Z=6.545）， fasting glucose （Z=3.506）， blood uric acid （Z=2.574）， urea nitrogen （Z=2.891）， creatinine （Z=2.243）， total bilirubin （Z=3.936）， glutathione （Z=0.969）， total cholesterol （t=3.956） and low density lipoprotein （Z=-5.678） were were statistically significant （P˂0.05 or 0.01）. Spearman correlation analysis showed that， the urine AD7c-NTP level was positively correlated with age and the levels of urea nitrogen， glucose， total cholesterol and low density lipoprotein （r=0.177， 0.178， 0.171， 0.109， 0.149， P˂0.01）， and negatively correlated with the level of total bilirubin （r=-0.172， P˂0.01）. Conclusionthe urine AD7c-NTP level in middle-aged and elderly females was signifitcantly higher than in middle-aged and elderly males.The urine AD7c-NTP level of middle-aged and elderly people was positively correlated with age， urea nitrogen， glucose， total cholesterol and low density lipoprotein， and negatively correlated with total bilirubin.
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Objective To investigate the drug resistant mechanism and the genetic homology of Acineto-bacter baumannii(A.ba)isolated from blood in the patients with bloodstream infection so as to provide a basis for the prevention and treatment of multi-drug resistant A.ba infection.Methods The different sites of sam-ples from the inpatients with bloodstream infection caused by A.ba were collected from January 2015 to June 2016.A total of 38 strains of A.Ba were isolated.The drug susceptibility test of all bacterial strains was per-formed according to the uniform operating specification.The carbapenemase gene was amplified by PCR and its products were analyzed by agarose gel electrophoresis.The homology study was performed by using multi-locus sequence typing(MLST)and eBURST analysis software.Results All 38 strains of A.ba were multi-drug-resistant.And in all of them,OXA-23-like and OXA-51-like gene,and ISAbal sequence were detected. OXA-23-like gene was associated with ISAbal sequence;the MLST typing showed that 38 A.ba strains mainly included the type ST195(55.3%),STn-2(15.8%)and STn-3(10.5%).The eBURST analysis indicated that the main prevalent clones were CC92,accounting for 86.8%.Conclusion Multi-drug resistant A.ba carries the same drug-resistant gene and its strain has higher genetic homology,indicating that the clone spread ex-ists,w hich provides a molecular biological basis for reducing the risks of A.ba related bloodstream infection in hospital.
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Objective:To lay a foundation of expressing VCTB fusion protein and preparation the oral vaccine for prophylaxis and therapy of H.pylori infection,the prokaryotic expression vectors contained the fusion gene vacA toxic subunit of H.pylori and cholera toxin subunit B(ctxB)was constructed.Methods:The high homogeneity gene fragment was found in comparing VacA toxic subunit gene of domestic typical H.pylori strains.The primers were designed according to vacA and ctxB sequences in the gene bank.VacA toxic subunit gene was amplified by PCR as the template of DNA genome of H.pylori and cloned into plasmid pQE30,which was plasmid pQE30-vacA.The ctxB gene was amplified by PCR as the template of pET32(?)+-ctxB plasmid.The purified ctxB gene was inserted into pQE30-vacA to construct expressing plasmid pQE-vctB of containing vacA and ctxB genes.pQE-vctB was transformed into prokaryotic E.coli Top10.The recombinant plasmid of bacteria cultivated was extracted and purified,and cutted with the incision enzyme to evaluated.Results:The sequence of vacA gene amplified was about 723 bp and the sequence of ctxB gene was about 372 bp.They were consistented with the anticipated length of the DNA.The expressing plasmid pQE-vctB was conformitied with objective gene inserted into pQE30.vctB fusion gene sequenced as 1 092 bp by the sequencing was conformitied with the genebank,and encodes polypeptides of 364 amino acid residues.Conclusion:The prokaryotic expression vectors contained vacA and ctxB fusion gene was constructed successfully and lay a foundation of expression VCTB fusion protein.