Determination of MDPV in human urine by liquid-phase small-extraction-GC/MS / 中国法医学杂志

Objective The method for the analysis of Methylenedioxypyrovalerone (MDPV) in urine by liquid-phase small-extraction- GC/MS have been developed and studied . Methods A 1-mL of urine sample was adjusted to pH9 with sodium bicarbonate, and was extracted with 50μL dichloromethane. The mixture centrifuged at 14000g for 2 min . A 1-μL organic phase was injected into the GC/MS system. Results The calibration curves showed good linearity in range of 0.05μg/mL~0.20μg/mL , and the limit of detection was 0.02μg/mL, and the RSD was 3.86~5.69%, and the recovery rate was 86.5~92.8 %. Conclusion The method is sensitive, accurate and was easy to operate for fast detection of MDPV in human urine.