Quality of defoamer enema combined with bellym assage on the colonic preparation in elderly patients with constipation / 中国实用护理杂志

Objective To explore the role of defoamer enema combined with bellym assage on the colonic preparation in elderly patients with constipation. Methods One hundred patients were divided into two groups by random number table method, the experimental group and the control group with 50 cases each. Patients in the control group were told to drink polyethyleneglycolelectrolytesolution 3000 ml. Patients in the experimental group were told to drink polyethyleneglycolelectrolytesolution 2000 ml. After medicinepre paration, the patients of experimental group were given defoamer enema. After that, they were undertaken counterclockwise massage for10 mins, then massageing clockwise until defecation. Results 14 patients with oral catharsis drugs failed to give up check, 46 cases of intervention group and 40 cases of control group finally complete intestinal preparation and colonoscopy. Intervention group patients after bowel preparation before the incidence of abdominal distension, abdominal pain were 6.52% (3/46), 8.70%(4/46), lower than the control group 65.00% (26/40), 25.00% (10/40), the difference was statistically significant (χ2= 32.74, 4.17, P< 0.05). Percent of pass was 65.22%(30/46) for intestinal preparation intervention group, significantly higher than the control group 35.00% (14/40), the difference was statistically significant (χ2= 7.82, P< 0.05). Intervention group intestinal cleanliness ratings of Ottawa total score was 4.00 (4.00), which was lower than the control group 7.00 (4.50), the difference was statistically significant (Z= 3.80, P< 0.05). Endoscopic check process, the intervention group arrived at the terminal ileum and mirror back time were 7.00 (3.00) and 9.00 (1.00) min, were less than 9.00(6.50) and 10.50 (3.00) min in the control group, the difference was statistically significant (Z= 2.09, 4.53, P< 0.05). Intervention group of colons polyps detection rate was 67.39% (31/46), higher than that of control group 30.00% (12/40), the difference was statistically significant (χ2 = 11.97, P< 0.05). Conclusions The bowel preparation with defoamer enema will enhance the intestinal tract cleaness and the detection rate of polyps in elderly patients with constipation.

Effects of arsenic trioxide on cell proliferation and apoptosis in C6 glioma cell / 中国医师进修杂志

Objective To investigate the effects of arsenic trioxide(As2O3) on the cell proliferation and apoptosis of C6 glioma cells in vitro.Methods The C6 glioma cells were treated by 1,3,5 μ mol/L of As2O3 with different duration and observed under the microscope and electromicroscope.The viability of C6 glioma cells was examined by methyl thiazolyl tetrazolium (MTT) assay,and cell cycle and apoptosis were examined by flow cytometry.Results After treatment of 1,3,5 μ mol/L As2O3,C6 glioma cells were inhibited obviously with a dose- and time-dependent manner (P ＜0.05) by MTT.During flow cytometry,more increasing apoptotic cells were found in different concentration As2O3.Characteristic morphological changes were observed in As2O3 intervention by transmission election microscopy including cell shrinkage,physaliphore,nuclear condensation and apoptosis and so on.Conclusion As2O3 can inhibit the cell proliferation and induce the apoptosis of C6 glioma cells.

Effects of quercetin on human laryngeal cancer Hep-2 cells / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To investigate the antiproliferative effects of quercetinon on human laryngeal cancer Hep-2 cells and its mechanism.@*METHOD@#The antiproliferative effects of quercetinon on human laryngeal cancer Hep-2 cells was evaluated by MTT assay. The morphological alterations were studied by inverted phase contrast microscope. The apoptosis and cell cycle of Hep-2 cells was analyzed by flow cytometry (FCW).@*RESULT@#The growth of Hep-2 cells was significantly inhibited by quercetin. By inverted phase contrast microscope, morphological alterations including adherencing capability weakening and shape shrinking were noticed. It was found that apoptosis cells increased with the increase of drug concentration and the elapse of time by FCW. Cell cycle transition arrested at S phase and the broken DNA was noticed.@*CONCLUSION@#Quercetin could effectively inhibit the proliferation of Hep-2 cells and its mechanism is probably related to the apoptosis.

Allograft renal transplantation improves chronic renal failure verified by the quality and quantity of erythrocytes / 中国组织工程研究

BACKGROUND: Repeated blood transfusion and recombinant human erythropoietin (rhEpo) have been previously used to improve anemia following chronic renal failure; however, their clinical applications are extremely limited by various side effects. OBJECTIVE: To investigate the therapeutic effect of allograft renal transplantation with rhEpo on anemia following chronic renal failure via verieying quality and quantity of some examines. DESIGN, TIME AND SETTING: A randomized grouping animal study was performed in Laboratory of Physiology. Shanxi Medical University from June 2004 to March 2005.MATERIALS: Eighty healthy adult male Wistar rats collected as receptors were injected with doxorubicin hydrochloride (6.5 mg/kg)into caudal vein three times per week for six weeks in total. Right kidney was then exsected to establish model of chronic renal failure. Another 20 newborn Wistar rats (3-4 days old, of both Sexes) were selected as donor. RhEpo (2000 U/mL) was provided by Shangdong Ahua Pharmaceutical Company (batch number-99435). METHODS: 20 adult rats in the control group did not undergo any treatments, and then other 60 adult rats were randomly divided into three groups of 20 rats per group after chronic renal failure modeling. Adult rats in the transplantation group underwent multidrop wansplantation of renal tissue into renal envelop; adult rats in the rhEpo group were intraperitoneally injected with rhEpo (30U/kg) three times Der week for six successive weeks in total; adult rats in the model group did not undergo any treatrnents. MAIN OUTCOME MEASURES: SABC assay was used to detect expression and distribution of rhEpo in reDal tissue and graft. ELISA was used to directly measure level of serum rhEpo in angular vein, routine assay was used to measure level of hemoglobin, and the corresponding kits were used to measure content of Na+-K+ATPaSe. activity of superoxide dismutase (SOD).and level of malondialdehyde (MDA)in erythrocyte membrane. RESULTS: rhEpo-antigen positive reaction showed a strongly positive expression in the control group. a weakly positive or an absent expression in the model group, and a strongly positive expression in the transplantation group. There were significant differences in the model group as compared to other two groups(P＜0.01).After 30,45,and 60 days, rhEpo level and numbers of hemoglobin and erythrocytes were higher in the rhEpo group and transplantation group than those in the model group(P＜0.05-0.01).After 60 days,rhEpo level in the rhEpo group was higher than that in the transplantation group (P＜0.05).On the other hand,after 30,45,and 60 days,MDA content in the rhEpo group and transplantation group was lower than that in the model group(P＜0.05).but there were no significant difeerences between rhEpo group and transplantation group(P＞0.05).Changes of SOD and Na+-K+ATPase were opposite to MDA among these groups.CONCLUSION:Renal transplantation can increase quality and quantity of erythrocytes of rats with anemia caused by chronic renal failure.The eflfect is equivalent to rhEpo.