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1.
Acta Medica Philippina ; : 95-102, 2023.
Artículo en Inglés | WPRIM | ID: wpr-988877

RESUMEN

Objectives@#We present in this article the design and evaluation of a blended learning approach for training community healthcare providers in performing newborn hearing screening (NHS).@*Methods@#We developed a blended learning course for training community healthcare providers on eHealth-enabled NHS, following Bloom’s revised taxonomy of educational objectives. The training involved three components: computer-based training (CBT), face-to-face (FTF) training, and on-site coaching. We used surveys and post-training interviews following Level 1 Kirkpatrick’s training evaluation model to get initial feedback on the training program. @*Results@#Thirty-one community healthcare providers from five rural health units and a private hearing screening center, with a mean age of 42.2 ± 12.0 years, participated in the pilot. 93.5% of the participants agreed that the program content met stated objectives and was relevant to their practice. The length of the course was perceived to be adequate. Overall satisfaction with the program was rated at 8.5 ± 0.9 (with ten as the highest). The majority expressed that the CBT and FTF course were satisfactory at 93.5% and 100%, respectively. All participants agreed that the course enhanced their knowledge of newborn hearing screening and telehealth. Positive reviews were received from participants on the use of CBT to improve theoretical knowledge before FTF training. Participants declared that FTF training and on-site coaching helped improved NHS skills and implementation. @*Conclusion@#Competent community healthcare providers are critical to strengthening the performance of the health system, and advances in the education and technology sectors offer promising potential in upskilling local healthcare providers. The increasing access of Filipino healthcare providers to improved information and communications technology (ICT) is a significant catalyst for pedagogical innovation, like the use of blended learning in the continuous professional development of health practitioners. As ICTs gradually penetrate the health sector, the challenge we now face is not whether but how we can use innovations in education strategies to benefit healthcare providers.


Asunto(s)
Recién Nacido , Telemedicina
2.
Acta Medica Philippina ; : 15-20, 2023.
Artículo en Inglés | WPRIM | ID: wpr-988868

RESUMEN

Background@#Universal newborn hearing screening is mandated in the Philippines through the Universal Newborn Hearing Screening and Intervention Act of 2009 (RA 9709). Newborn hearing screening (NBHS) centers are required to perform screening tests, compile and submit data on screened newborns, and advise parents on the subsequent steps after NBHS.@*Objective@#The study aimed to conduct a survey of the implementation of the Universal Newborn Hearing Screening and Intervention Program (UNHSIP) in the different regions of the country; and assess the information technology (IT) capabilities of hearing centers.@*Methods@#Fifty-one NBHS centers across twelve regions were surveyed through on-site inspections in 2016. Data was gathered on the centers’ testing capability, staffing, access to specialists, use of local protocols, connectivity, and IT capabilities. @*Results@#All surveyed centers followed the recommended protocols of the Manual of Operations of the Universal Newborn Hearing Screening and Intervention Act of 2009 (RA 9709). Among the 12 regions visited, only five (41.67%) had Category C centers with confirmatory testing and early amplification services as recommended. Majority of facilities (96.1%) were staffed by trained and certified personnel. A small percentage had access to subspecialists such as clinical audiologists (39.2%) and speech-language pathologists (23.5%). All facilities had computer access, but only 58.8% had internet access. Majority (94.1%) of the centers visited were not using the recommended data submission methods, specifically the use of registry cards and the online registry. Only 27.5% of centers had data on newborns who underwent confirmatory testing or early intervention. @*Conclusion@#Facilities were found to be compliant to NBHS screening protocols and majority complied with certification requirements for staff; but were found to be non-compliant with use of registry cards or the online registry. Majority of centers were able to contact the parents of neonates who did not pass newborn screening, but had no system to track outcomes. Lack of confirmatory and early intervention services in identified areas emphasize the need for development of regional centers. It is recommended that measures to improve the utilization of the online registry are taken.


Asunto(s)
Tamizaje Neonatal
3.
Philippine Journal of Surgical Specialties ; : 87-91, 2021.
Artículo en Inglés | WPRIM | ID: wpr-964551

RESUMEN

@#The authors report a case of post-tonsillectomy dysgeusia and discuss the pathogenesis, diagnostics, as well as treatment options done in several reported cases. A 37-year-old man who was diagnosed with recurrent tonsillitis underwent bilateral palatine tonsillectomy, and on the second post-operative day, post-tonsillectomy hemorrhage ensued which required emergency hemostasis at the operating room. Intra-operative findings include active bleeding on the left tongue base, wherein hemostasis was achieved via electrodissection. After the procedure, patient noted a disturbance to taste that persisted for several months. Dysgeusia is an unusual complication of tonsillectomy, occurring in 0.3% to 9% of cases.


Asunto(s)
Tonsilectomía , Disgeusia
4.
Philippine Journal of Otolaryngology Head and Neck Surgery ; : 37-40, 2017.
Artículo en Inglés | WPRIM | ID: wpr-961004

RESUMEN

@#<p style="text-align: justify;"><strong>OBJECTIVES:</strong> To discuss a rare case of temporal bone capillary hemangioma and its diagnosis and management.<br /><strong>METHODS:</strong><br /><strong>Design:</strong> Case Report<br /><strong>Setting:</strong> Tertiary Government Hospital<br /><strong>Patient:</strong> One<br /><strong>RESULTS</strong>: A 44-year-old woman with a history of on-and-off right ear discharge, tinnitus and decreased hearing, and a pinkish, smooth-surfaced, non-friable, non-pulsating mass occluding the right external auditory canal, was initially treated for chronic suppurative otitis media with aural polyp. A punch biopsy due to persistence of disease despite medical treatment revealed capillary hemangioma. She underwent canal wall down mastoidectomy with obliteration to completely resect the tumor.<br /><strong>CONCLUSION:</strong> Capillary hemangiomas of the temporal bone are benign lesions that may lead to complications such as bone erosion, hearing loss, recurrent infection and bleeding if left untreated. Surgery remains the ideal treatment and recurrence is rare and the prognosis is good if resection is complete.</p>


Asunto(s)
Humanos , Femenino , Persona de Mediana Edad , Conducto Auditivo Externo , Otitis Media Supurativa , Acúfeno , Pérdida Auditiva , Hueso Temporal , Sordera , Apófisis Mastoides , Hemangioma Capilar , Pronóstico , Biopsia , Pólipos
5.
Acta Medica Philippina ; : 28-35, 2017.
Artículo en Inglés | WPRIM | ID: wpr-959857

RESUMEN

@#<p style="text-align: justify;"><strong>BACKGROUND:</strong> In 2000, the World Health Organization recommended implementation of universal hearing screening. The Philippines enacted this policy into law in 2009 as it was found to be cost-effective for the Philippines. The model at the time used a hospital-based approached to screening. This paper examines the cost-effectiveness and budget impact of implementing a community-based universal hearing screening program.<br /><strong>METHODS:</strong> A model was developed following a community-based hearing screening program. Parameters were obtained through literature review, secondary data analysis, and consultation with experts. Cost-effectiveness was assessed for a single birth cohort from a public payer and societal perspective.<br /><strong>RESULTS:</strong> A community-based universal hearing screening program was found to be cost-saving. One-way sensitivity analysis showed that results were sensitive only to treatment rate and follow-up rate. The program is also a high budget impact program.<br /><strong>CONCLUSION:</strong> A community-based hearing screening program is cost-saving for the Philippines. Ensuring treatment and good follow-up in testing will ensure cost-effectiveness.</p>


Asunto(s)
Pérdida Auditiva , Análisis de Costo-Efectividad
6.
Acta Medica Philippina ; : 61-64, 2017.
Artículo | WPRIM | ID: wpr-959836

RESUMEN

BACKGROUND: Medication errors are preventable events that can cause or lead to inappropriate drug use. Knowing the prevalence and types of errors can help us institute corrective measures and avoid adverse drug events.OBJECTIVE: This study determined the prevalence of medication errors and its specific types in the four main service wards of a tertiary government training medical center.METHODS: This is a retrospective, descriptive chart review study. From the master list of admissions, systematic sampling was done to retrieve the required number of charts. Relevant pages such as order sheets, nurses' notes, therapeutic sheets were photographed. For prolonged admissions, only the first 7 days were reviewed. Each chart was evaluated by two people who then met and agreed on the errors identified.RESULTS: The overall prevalence of medication errors is 97.8%. Pediatrics had the most (63.3/chart), followed by Medicine, OB-Gynecology, and Surgery (7.3/chart). The most common type of errors identified were prescribing, followed by compliance, then administration errors.CONCLUSION: Medication errors are present in the four main wards in our hospital. We recommend orientation of all incoming first year residents on proper ordering and prescribing of drugs, as well as a prospective observational study to determine true prevalence of all types of medication errors.


Asunto(s)
Humanos , Masculino , Femenino , Preparaciones Farmacéuticas , Ginecología , Prescripción Inadecuada , Efectos Colaterales y Reacciones Adversas Relacionados con Medicamentos , Hospitales , Pediatría
7.
Philippine Journal of Otolaryngology Head and Neck Surgery ; : 37-40, 2017.
Artículo en Inglés | WPRIM | ID: wpr-633856

RESUMEN

OBJECTIVES: To discuss a rare case of temporal bone capillary hemangioma and its diagnosis and management.METHODS:Design: Case ReportSetting: Tertiary Government HospitalPatient: OneRESULTS: A 44-year-old woman with a history of on-and-off right ear discharge, tinnitus and decreased hearing, and a pinkish, smooth-surfaced, non-friable, non-pulsating mass occluding the right external auditory canal, was initially treated for chronic suppurative otitis media with aural polyp. A punch biopsy due to persistence of disease despite medical treatment revealed capillary hemangioma. She underwent canal wall down mastoidectomy with obliteration to completely resect the tumor.CONCLUSION: Capillary hemangiomas of the temporal bone are benign lesions that may lead to complications such as bone erosion, hearing loss, recurrent infection and bleeding if left untreated. Surgery remains the ideal treatment and recurrence is rare and the prognosis is good if resection is complete.


Asunto(s)
Humanos , Femenino , Persona de Mediana Edad , Conducto Auditivo Externo , Otitis Media Supurativa , Acúfeno , Pérdida Auditiva , Hueso Temporal , Sordera , Apófisis Mastoides , Hemangioma Capilar , Pronóstico , Biopsia , Pólipos
8.
Acta Medica Philippina ; : 61-64, 2017.
Artículo en Inglés | WPRIM | ID: wpr-633383

RESUMEN

@#<p style="text-align: justify;"><strong>BACKGROUND:</strong> Medication errors are preventable events that can cause or lead to inappropriate drug use. Knowing the prevalence and types of errors can help us institute corrective measures and avoid adverse drug events.<br /><strong>OBJECTIVE:</strong> This study determined the prevalence of medication errors and its specific types in the four main service wards of a tertiary government training medical center.<br /><strong>METHODS:</strong> This is a retrospective, descriptive chart review study. From the master list of admissions, systematic sampling was done to retrieve the required number of charts. Relevant pages such as order sheets, nurses' notes, therapeutic sheets were photographed. For prolonged admissions, only the first 7 days were reviewed. Each chart was evaluated by two people who then met and agreed on the errors identified.<br /><strong>RESULTS:</strong> The overall prevalence of medication errors is 97.8%. Pediatrics had the most (63.3/chart), followed by Medicine, OB-Gynecology, and Surgery (7.3/chart). The most common type of errors identified were prescribing, followed by compliance, then administration errors.<br /><strong>CONCLUSION:</strong> Medication errors are present in the four main wards in our hospital. We recommend orientation of all incoming first year residents on proper ordering and prescribing of drugs, as well as a prospective observational study to determine true prevalence of all types of medication errors.</p>


Asunto(s)
Errores de Medicación
9.
Philippine Journal of Otolaryngology Head and Neck Surgery ; : 31-35, 2016.
Artículo en Inglés | WPRIM | ID: wpr-632649

RESUMEN

@#<p style="text-align: justify;"><strong>OBJECTIVE:</strong> To present an unusual cause of pulsatile tinnitus, presenting in a young adult suffering from chronic recurrent foul-smelling discharge from the same ear.<br /><strong>METHODS:</strong><br /><strong>Design:</strong> Case Report<br /><strong>Setting:</strong> Tertiary National University Hospital<br /><strong>Patient:</strong> One<br /><strong>RESULTS:</strong> A 24 year-old woman presented with pulsatile tinnitus on a background of chronic recurrent foul-smelling discharge. Clinico-radiologic findings seemed consistent with a glomus tympanicum coexisting with chronic suppurative otitis media with cholesteatoma. She underwent tympanomastoidectomy with excision of the mass. Histopathologic evaluation revealed the mass to be granulation tissue.<br /><strong>CONCLUSION:</strong> Pulsatile tinnitus is rarely associated with chronic middle ear infection. Granulation tissue arising at the promontory may mimic glomus tumors when accompanied with this symptom. Despite this revelation, it would still be prudent to prepare for a possible glomus tumor intraoperatively so that profuse bleeding and complications may be avoided.</p>


Asunto(s)
Humanos , Femenino , Adulto Joven , Dolor de Oído , Cefalea , Vértigo , Tumor Glómico , Colesteatoma
10.
Acta Medica Philippina ; : 30-34, 2014.
Artículo en Inglés | WPRIM | ID: wpr-633734

RESUMEN

BACKGROUND AND AIMS: Acute otitis media (AOM) seems to be a significant public health problem, but national data on its prevalence is lacking. This study aims to determine the prevalence of acute otitis media in the Philippines and characterize this population.METHODS: A cross-sectional survey of children 0-12 years old in community health centers and schools was performed, utilizing a multi-stage cluster sampling design.RESULTS: Thirteen areas were included in the survey with 26 household and school clusters, surveying a total of 2,277 children. There were 218 children with clinically diagnosed acute otitis media, with an overall prevalence rate of 9.6%. Among the children with AOM, 108 (10%) were male while 110 (9.2%) were female. There were 32 (14.6%) cases of AOM in the 0-2 year olds, 56 (9.5%) in the 3-6 year olds, and 117 (8.4%) in the 7-12 year old children.CONCLUSIONS: The overall prevalence of clinically diagnosed acute otitis media in the Philippines is 9.6%, with a variables area-specific prevalence. There is no gender predilection, with the 0-2 age group having the most prevalent cases of AOM in the sample.


Asunto(s)
Humanos , Masculino , Femenino , Niño , Lactante , Otitis Media , Inflamación , Oído , Prevalencia , Diagnóstico , Enfermedad Aguda , Centros Comunitarios de Salud
11.
Philippine Journal of Otolaryngology Head and Neck Surgery ; : 5-9, 2011.
Artículo en Inglés | WPRIM | ID: wpr-632434

RESUMEN

@#<p style="text-align: justify;"><strong>OBJECTIVES</strong>: This study aimed to determine the prevertebral soft tissue thickness among normal patients aged 0-14 years old in a tertiary government training hospital, to compare these values with divergent criteria in the standard otorhinolaryngology and radiology texts used in our institution, and to recommend adoption of a set of criteria based on the results.<br /><br /><strong>METHODS</strong>:<br /><strong>Design</strong>: Descriptive Study <br /><strong>Setting</strong>: Tertiary Government Hospital<br /><strong>Subjects and Methods</strong>: Lateral cervical radiographs taken from May 2007 to August 2009 which were initially read as normal were collected. Fifty (50) patients, 39 males and 11 females, aged 0-14 years old meeting inclusion criteria were reviewed and prevertebral soft tissue thicknesses (PVST) and cervical vertebral body diameter at levels C2, C5, C6 were measured and compared to criteria set by standard otorhinolaryngology and radiology textbooks.<br /><br /><strong>RESULTS</strong>: The average PVST at C2 ranged from 4.02 mm for 2-3 year-olds (n= 2) to 8.16 mm for 1 -2 year-olds (n=2). The average PVST at C5 ranged from 8.11mm for 1-2 year-olds to 10.75 mm for for 0-1 year-olds. The average PVST at C6 ranged from 7.13 mm for 1 - 2 year-olds to 10.36 mm for 0-1 year-olds. Only 12% of the patients satisfied the criteria set by Keats and Lusted, while 100% satisfied Duncan's criteria, 94% and 98% satisfied Wippold's first and second criteria respectively. <br /><br /><strong>CONCLUSION</strong>: All of the PVST criteria mentioned in Cummings' Textbook of Otorhinolaryngology Head and Neck Surgery had a more than 90% accuracy compared to only 12% for those mentioned in Keats and Lusted's Atlas of Roentgenographic Measurement. Therefore, we recommend the use of any criteria for PVST contained in the former over the latter.</p>


Asunto(s)
Humanos , Masculino , Femenino , Adolescente , Niño , Lactante , Columna Vertebral , Radiografía , Huesos
12.
Rev. bras. farmacogn ; 20(6): 891-896, dez. 2010. tab
Artículo en Portugués | LILACS | ID: lil-572620

RESUMEN

Pterodon emarginatus Vogel, Fabaceae, conhecida popularmente como sucupira-branca, é uma espécie arbórea que possui interesse como planta medicinal e fonte de madeira. Vários estudos com o óleo e extratos dos seus frutos demonstraram atividades cercaricida, antimicrobiana e antiinflamatória. Tendo em vista a importância de P. emarginatus como planta medicinal o presente trabalho analisou a composição química, a atividade antifúngica e antibacteriana do óleo essencial de suas folhas, isolou e identificou compostos do extrato etanólico bruto das folhas. A análise do óleo essencial das folhas permitiu a identificação de 9 hidrocarbonetos sesquiterpênicos, sendo os majoritários o γ-muuroleno (48,79 por cento) e o biciclogermacreno (22,66 por cento). O óleo essencial apresentou atividade frente a bactérias Gram-positivas, sendo inativo sobre isolados clínicos de Candida. O estudo fitoquímico do extrato etanólico das folhas permitiu o isolamento da mistura dos esteróides estigmasterol e β-sitosterol. Todos os resultados obtidos contribuem para a ampliação de informações sobre essa planta amplamente utilizada pela população.


Pterodon emarginatus Vogel, Fabaceae, known as "sucupira-branca", is a tree specie with interest as medicinal plant and source of wood. Several studies with the oil and fruit's extracts have been showed cercaricid, antimicrobial and antiinflamatory activities. The importance of P. emarginatus conducted the present study to realize evaluated antifungal and antibacterial activity of essential oil of their leaves, and isolated some compounds of ethanolic extract of the leaves. The analysis of the essential oil from leaves allowed the identification of nine sesquiterpenics hydrocarbons, with the majority γ-muurolene (48.79 percent) and biciclogermacrene (22.66 percent). The essential oil showed activity against the Gram-positive bacteria, and inactive on clinical isolates of Candida. The phytochemical study of the ethanol extract of the leaves allowed the isolation of the mixture of stigmasterol and β-sitosterol steroids. All results contribute to the expansion of information about this plant widely used by the population.

13.
Philippine Journal of Surgical Specialties ; : 74-80, 2009.
Artículo en Inglés | WPRIM | ID: wpr-732097

RESUMEN

OBJECTIVE: The aim of the study was to describe the diagnosissurgical management and outcomes of eight patients with bone paraganglioma.PATIENTS: A series of 8 patients with temporal bone paraganglioma were seen over a 10-year period and operated n by the senior author.RESULTS: The majority of patients presented with hearing loss and pulsatile tinnitus. Other symptoms were facial numbness, facial asymmetry, dizziness, hoarseness, and dysphagia. All patients had pre-operative high resolution computed temography (HRCT) scan and/or magnetic resonance imaging. Pre-operative angiography and embolization facilitated the excision of these tumors in all but one case. There was reversal of pre-existent cranial nerve neuropathy in 3 of 8 cases. Unique to this series were the management of a patient who previously underwent gamma knife radiosurgery, one case in whom the glomus tumor grew from the better hearing ear thus posing the surgical management challenges and a complicated case of postoperative necrosis that required latissimus dorsi flap reconstruction. Other complications were described including post-operative facial paresis which eventually resolved in all patients.CONCLUSION: In our setting, it is possible to achieve good result with acceptable morbidity with surgery with or without postoperative radiotherapy.


Asunto(s)
Humanos , Acúfeno , Mareo , Tumor Glómico , Parálisis Facial , Hipoestesia , Ronquera , Audición , Pérdida Auditiva , Hueso Temporal , Paraganglioma , Angiografía , Nervios Craneales
14.
Philippine Journal of Otolaryngology Head and Neck Surgery ; : 27-31, 2009.
Artículo en Inglés | WPRIM | ID: wpr-633256

RESUMEN

Objective:To describe an intranasal mass initially diagnosed and treated as benign that eventually turned out to be a malignant extramedullary plasmacytoma of the maxillary sinus and to review the literature on its presenting signs and symptoms, diagnosis, management and pathophysiology. Methods: Design: Case Report Setting: Tertiary Public Hospital Patient: One Results: A 45-year-old male with persistent nasal obstruction and intermittent epistaxis underwent several biopsies of a mass shown on computed tomography scans as heterogeneously enhancing, expansile, occupying the left maxillary sinus with extension into the left nasal cavity with areas of erosion. Immunohistochemical staining was negative for cytokeratin (CK) and leukocyte common antigen (LCA). Complete excision yielded a final histopathologic interpretation of plasmacytoma. Laboratory examinations excluded multiple myeloma. The final diagnosis was extramedullary plasmacytoma and he was treated with post-operative adjuvant radiotherapy. Conclusion: Plasmacytoma may present in the sinu-nasal region and be part of a systemic disease like multiple myeloma. A high index of suspicion and thorough initial histopathological work-up may help in establishing a definitive diagnosis and providing optimum treatment.

15.
P. R. health sci. j ; 25(2): 159-161, Jun. 2006.
Artículo en Inglés | LILACS | ID: lil-472185

RESUMEN

The authors describe a pediatric patient who presented with a 3-month history of dry cough, chest pain, progressive breathlessness, fever and recurrent pneumonia with atelectasis. A fiberoptic bronchoscopy revealed a whitish lesion at the left bronchus. A biopsy of the lesion demonstrated an anaplastic large cell lymphoma (ALCL). Evaluation for disseminated disease was negative. After the patient completed chemotherapy the lesion abated and she has been in complete remission for almost 4 years. Although extranodal involvement of ALCL is frequent at some stage of the disease, endobronchial involvement is extremely rare even in the presence of advanced disease. To our knowledge, this is the first primary isolated endobronchial ALCL described in a pediatric patient.


Asunto(s)
Humanos , Femenino , Niño , /administración & dosificación , Neoplasias de los Bronquios , Linfoma de Células B Grandes Difuso , Antibióticos Antineoplásicos , Antimetabolitos Antineoplásicos/administración & dosificación , Antineoplásicos Fitogénicos/administración & dosificación , Antineoplásicos Hormonales/administración & dosificación , Biopsia , Broncoscopía , Bronquios/patología , Doxorrubicina/administración & dosificación , Estudios de Seguimiento , Inmunosupresores , Inyecciones Intravenosas , Inyecciones Espinales , Metotrexato , Prednisona/administración & dosificación , Protocolos de Quimioterapia Combinada Antineoplásica/uso terapéutico , Radiografía Torácica , Inducción de Remisión , Tomografía Computarizada de Emisión de Fotón Único , Tomografía Computarizada por Rayos X , Vincristina/administración & dosificación
16.
Rev. Inst. Med. Trop. Säo Paulo ; 47(1): 55-57, jan.-fev. 2005. ilus
Artículo en Inglés | LILACS | ID: lil-393344

RESUMEN

Este trabalho relata um caso de feohifomicose subcutânea causado por Exophiala jeanselmei em um paciente que havia recebido transplante de coração e mantinha terapia com micofenolato mofetil, tracolimus e prednisone. As lesões tiveram início após trauma na perna inferior direita que evoluíram produzindo múltiplos nódulos e úlceras. Diagnóstico foi realizado através de avaliação histológica e de características macroscópicas e microscópicas da cultura das lesões da pele. O paciente fez uso de itraconazol em concentração de 200 mg/dia durante três meses, não se observando no entanto, melhora das lesões. Após este período, o paciente foi tratado com anfotericina B a uma concentração de 0,5 mg/Kg/dia totalizando 3,8 g. Após quatro meses de tratamento as lesões mostraram melhora evidente, verificando-se fechamento das fístulas e cicatrização das lesões.


Asunto(s)
Humanos , Masculino , Persona de Mediana Edad , Dermatomicosis/microbiología , Exophiala , Trasplante de Corazón , Huésped Inmunocomprometido , Anfotericina B/uso terapéutico , Antifúngicos/uso terapéutico , Dermatomicosis/tratamiento farmacológico , Dermatomicosis/patología , Inmunosupresores/uso terapéutico , Itraconazol/uso terapéutico , Tejido Subcutáneo/microbiología
17.
Mem. Inst. Oswaldo Cruz ; 98(7): 963-965, Oct. 2003. tab
Artículo en Inglés | LILACS | ID: lil-352402

RESUMEN

The essential oil and the aqueous, hexane and methanolic fractions from Hyptis ovalifolia leaves were evaluated for their antifungal activity in vitro against 60 strains of dermatophytes: 10 strains of Microsporum canis, 10 of M. gypseum, 20 of Trichophyton rubrum and 20 of T. mentagrophytes. The extracts inhibited growth of the dermatophytes tested at different concentrations. The most biologically active was the essential oil from the leaves which inhibited 57 isolates (95 percent) at a concentration of ú 500 æg/ml.


Asunto(s)
Antifúngicos , Arthrodermataceae , Relación Dosis-Respuesta a Droga , Pruebas de Sensibilidad Microbiana , Extractos Vegetales
18.
Mem. Inst. Oswaldo Cruz ; 98(6): 839-841, Sept. 2003.
Artículo en Inglés | LILACS | ID: lil-348356

RESUMEN

Sixty clinical isolates of Cryptococcus neoformans from AIDS from Goi⮩a, state of Goiᳬ Brazil, were characterized according to varieties, serotypes and tested for antifungal susceptibility. To differentiate the two varieties was used L-canavanine-glycine-bromothymol blue medium and to separate the serotypes was used slide agglutination test with Crypto Check Iatron. The Minimal Inhibitory Concentration (MIC) of fluconazole, itraconazole, and amphotericin B were determined by the National Committee for Clinical Laboratory Standards macrodilution method. Our results identified 56 isolates as C. neoformans var. neoformans serotype A and 4 isolates as C. neoformans var. gattii serotype B. MIC values for C. neoformans var. gattii were higher than C. neoformans var. neoformans. We verified that none isolate was resistant to itraconazole and to amphotericin B, but one C. neoformans var. neoformans and three C. neoformans var. gattii isolates were resistant to fluconazole. The presence of C. neoformans var. gattii fluconazole resistant indicates the importance of determining not only the variety of C. neoformans infecting the patients but also measuring the MIC of the isolate in order to properly orient treatment


Asunto(s)
Adulto , Persona de Mediana Edad , Anfotericina B , Antifúngicos , Cryptococcus neoformans , Fluconazol , Itraconazol , Pruebas de Aglutinación , Infecciones Oportunistas Relacionadas con el SIDA , Cryptococcus neoformans , Pruebas de Sensibilidad Microbiana , Serotipificación
19.
Rev. Inst. Med. Trop. Säo Paulo ; 44(3): 121-125, 2002. tab
Artículo en Inglés | LILACS | ID: lil-314538

RESUMEN

A comparison of the Etest and the reference broth macrodilution susceptibility test for fluconazole, ketoconazole, itraconazole and amphotericin B was performed with 59 of Candida species isolated from the oral cavities of AIDS patients. The Etest method was performed according to the manufacturer's instructions, and the reference method was performed according to National Committee for Clinical Laboratory Standards document M27-A guidelines. Our data showed that there was a good correlation between the MICs obtained by the Etest and broth dilution methods. When only the MIC results at ± 2 dilutions for both methods were considered, the agreement rates were 90.4 percent for itraconazole, ketoconazole and amphotericin B and 84.6 percent for fluconazole of the C. albicans tested. In contrast, to the reference method, the Etest method classified as susceptible three fluconazole-resistant isolates and one itraconazole-resistant isolate, representing four very major errors. These results indicate that Etest could be considered useful for antifungal sensitivity evaluation of yeasts in clinical laboratories


Asunto(s)
Humanos , Antifúngicos , Candida , Pruebas de Sensibilidad Microbiana , Fluconazol , Itraconazol
20.
Rev. Soc. Bras. Med. Trop ; 33(1): 75-78, jan.-fev. 2000. tab, graf
Artículo en Portugués | LILACS | ID: lil-301714

RESUMEN

Criptococose é considerada a infecçäo fúngica sistêmica oportunista mais comum em pacientes com AIDS. Nestes pacientes tem predominado como agente etiológico Cryptococcus neoformans var. neoformans, e muito raramente relata-se C. neoformans var. gattiii, mesmo nas regiöes onde se verifica a sua prevalência. Foram estudados 50 pacientes com lesöes de criptococose meningoencefálica associada com AIDS. Os isolados foram identificados através de características microscópicas e macroscópicas exibidas em meios de ágar Sabouraud, ágar niger e Christensen. As variedades de C. neoformans foram determinadas pela reaçäo de coloraçäo obtida em meio de L-canavanina glicina-azul de bromotimol (CGB). Em todos os pacientes examinados foram isolados C. neoformans, sendo identificados C. neoformans var. neoformans em 47 isolados e C. neoformans var. gattii em 3. Os resultados encontrados mostram que a criptococose em pacientes com AIDS pode também ser causada por C. neoformans var. gatti, apesar de haver predominância de C. neoformans var neoformans nesta populaçäo


Asunto(s)
Cryptococcus neoformans
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