Pollution of drinking water with coliform a pilot study

This study was carried out on 200 water samples, 100 samples collected from Aga [Dakahilia Governorate] which represent the cholorinated water, the other 100 samples collected form Minet Samanoud Village [Gharbia Governorate] which represent the underground water. Out of 100 chlorinated water samples, 10 samples [10%] were poluted. The pollution with typical coliform represented In 2 samples [2%] while 8% non faecal coliform [atypical]. Pollution with coliform bacilli in the underground water was 42% out of 100 water samples, 30 samples [30%] were polluted with typical coliform bacilli while 12 samples [12%] were polluted with atypical type. Water samples were colIected by special method from different sites. All samples were subjected to comprehensive bacteriological study including routine tests for faecal pollution as plate count, presumptive coliform count [MPN] and differential coliform test Including Eijkman test and citrate utilisation test. Our work intended to evaluate the system of purification and control of water to reduce the rate of pollution. From this study, it can be concluded that Pollution with typical coliform bacilli was heavily in the underground water. We also recommend continouous and frequent cleaning of storage tanks and sahreigs which may be responsible for pollution

effect of Nigella sativa [black seed] on the B cell function of balb/C mice

The genus Nigella includes many species [N. sativa L., N. arensis L., N. assyriace Boiss., N. deserti Boiss., N. hispanica L., and N. damascena L., amongst which only N. sativa L. is known to be indogenous and grows well in different localities of Egypt. This species is known to have considerable therapeutic values. The immunological effect of this seed on the immune system was studied by testing the B cell function of BALB/C mice after their feeding on the seed for two weeks. The test was performed by the method of plaque assay using Cunningham and Szenberg slides. The results was so encouraging because it revealed a four fold increase in the mean number of plaques in mice fed on the seeds [80 plaques per 10 lymphocytes] while those not fed on the seeds [i.e.] control group showed only 20 plaques per 10 lymphocytes