The use of frozen inoculum and 0,2 por cento formaldehyde for inactivation of Pertussis vaccine production

The use of frozen seeds and the effect of the concentration of formaldehyde, and the removal of 2/3 of the supernatant were investigated in order to facilitate the production of pertussis cellular vaccine. Results indicated that is possible to replace fresh seeds by frozen ones, and the formaldehyde concentration can be increased to 0,2 por cento after the remotion of 2/3 of supernatant, resulting in a good vaccine preparation in a shorter time