Partial Optimization and Study of Antimicrobial Activity of Polyphenol Oxidase (PPO) and Peroxidase (POD) Extracted from Chilly Pepper Pericarp.

Polyphenol oxidase(PPO) (E.C. number 1.10.3.1) has ubiquitous distribution in almost all living organism. Whereas, peroxidase(POD) (E.C. number 1.11.1) act as hormone regulation and defense mechanism in plants. Keeping in pace with their present-day industrial application, efforts have been made to evaluate the activity of these two enzymes (PPO and POD) using pepper pericarp (Capsicum annuum L.) as an experimental material using catechol and guaiacol as a substrate, respectively. The effects of enzyme extract, substrate, hydrogen peroxide concentration (only for POD), pH and temperature and antimicrobial activity against different bacterial strains were investigated.