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Mem. Inst. Oswaldo Cruz ; 100(3): 281-283, May 2005. ilus
Artículo en Inglés | LILACS | ID: lil-411025

RESUMEN

Trypanosoma cruzi is classified into two major groups named T. cruzi I and T. cruzi II. In the present work we analyzed 16 stocks isolated from human cases and four isolated from triatomines from diverse geographical origins (Mexico and Guatemala). From human cases four were acute cases, six indeterminates, and six from chronic chagasic cardiophatic patients with diagnosis of dilated cardiomyopathy established based on the left-ventricular end systolic dimension and cardiothoracic ratio on chest X-radiography and impaired contracting ventricle and different degree conduction/rhythm aberrations. DNA samples were analyzed based on mini-exon (ME) polymorphism, using a pool of three oligonucleotide for the amplification of specific intergenic region of T. cruzi ME gene. All the Mexican and Guatemalan isolates regardless their host or vector origin generated a 350 bp amplification product. In conclusion T. cruzi I is dominant in Mexico and Guatemala even in acute and chronic chagasic cardiopathy patients. To our knowledge, this is the first study describing predominance of T. cruzi I in human infection for North and Central America.


Asunto(s)
Animales , Humanos , Cardiomiopatía Chagásica/parasitología , ADN Protozoario/análisis , Trypanosoma cruzi/genética , Enfermedad Aguda , Enfermedad Crónica , Guatemala , México , Reacción en Cadena de la Polimerasa , Análisis de Secuencia de ADN , Triatominae/parasitología , Trypanosoma cruzi/clasificación , Trypanosoma cruzi/aislamiento & purificación
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