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Chinese Journal of Applied Physiology ; (6): 453-457, 2010.
Artículo en Chino | WPRIM | ID: wpr-301538

RESUMEN

<p><b>OBJECTIVE</b>To investigate the protective effect of Crocin against hypoxia damage of cardiac myocytes of neonatal rats and the regulation of HIF-1 and prolyhydroxylase (PHDs).</p><p><b>METHODS</b>A model of CoCl2 simulated hypoxia damage was established in primary cultural myocardial cell. Expression levels of HIF-1alpha, VEGF, iNOS, as well as PHD1, 2, 3 protein in myocardial cells were detected by Western blot.</p><p><b>RESULTS</b>Compared with CoCl2 group, the viability of myocardial cell was significantly increased after treated 24 h at 10(-5)mol/L Crocin (P < 0.01), HIF-1alpha, VEGF and iNOS were expressed higher than those in Crocin + CoCl2 group (P < 0.01), the expression of PHD2 was significantly increased (P < 0.01), while the expression of PHD3 was remarkably reduced in Crocin + CoCl2 Group (P < 0.01).</p><p><b>CONCLUSION</b>Crocin has better protective effect on hypoxic damage of myocardial cell. The mechanisms of protective effect of Crocin may be related to the activation of HIF-1-mediated pathway of the hypoxia response. PHDs may be involved in the pathophysiology regulated process of myocardial cells.</p>


Asunto(s)
Animales , Ratas , Carotenoides , Farmacología , Hipoxia de la Célula , Células Cultivadas , Proteínas de Homeodominio , Metabolismo , Subunidad alfa del Factor 1 Inducible por Hipoxia , Metabolismo , Prolina Dioxigenasas del Factor Inducible por Hipoxia , Metabolismo , Miocitos Cardíacos , Metabolismo , Óxido Nítrico Sintasa de Tipo II , Metabolismo , Procolágeno-Prolina Dioxigenasa , Metabolismo , Ratas Sprague-Dawley , Factor A de Crecimiento Endotelial Vascular , Metabolismo
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