RESUMEN
Our previous studies showed that biomodification of demineralized dentin collagen with proanthocyanidin (PA) for a clinically practical duration improves the mechanical properties of the dentin matrix and the immediate resin-dentin bond strength. The present study sought to evaluate the ability of PA biomodification to reduce collagenase-induced biodegradation of demineralized dentin matrix and dentin/adhesive interfaces in a clinically relevant manner. The effects of collagenolytic and gelatinolytic activity on PA-biomodified demineralized dentin matrix were analysed by hydroxyproline assay and gelatin zymography. Then, resin-/dentin-bonded specimens were prepared and challenged with bacterial collagenases. Dentin treated with 2% chlorhexidine and untreated dentin were used as a positive and negative control, respectively. Collagen biodegradation, the microtensile bond strengths of bonded specimens and the micromorphologies of the fractured interfaces were assessed. The results revealed that both collagenolytic and gelatinolytic activity on demineralized dentin were notably inhibited in the PA-biomodified groups, irrespective of PA concentration and biomodification duration. When challenged with exogenous collagenases, PA-biomodified bonded specimens exhibited significantly less biodegradation and maintained higher bond strengths than the untreated control. These results suggest that PA biomodification was effective at inhibiting proteolytic activity on demineralized dentin matrix and at stabilizing the adhesive/dentin interface against enzymatic degradation, is a new concept that has the potential to improve bonding durability.
Asunto(s)
Humanos , Clorhexidina , Química , Farmacología , Colagenasas , Farmacología , Recubrimiento Dental Adhesivo , Cementos Dentales , Química , Análisis del Estrés Dental , Dentina , Recubrimientos Dentinarios , Química , Gelatinasas , Farmacología , Hidroxiprolina , Metaloproteinasa 8 de la Matriz , Farmacología , Inhibidores de la Metaloproteinasa de la Matriz , Química , Farmacología , Proantocianidinas , Química , Farmacología , Estrés Mecánico , Propiedades de Superficie , Resistencia a la Tracción , Desmineralización Dental , PatologíaRESUMEN
<p><b>OBJECTIVE</b>To investigate the potential effect of proanthocyanidins (PA), a natural cross-linker, on the stability of resin-dentin bonds against thermal cycling.</p><p><b>METHODS</b>Ten percent, 15% PA-based preconditioners, and 5% glutaraldehyde were prepared for the transient pretreatment of demineralized dentin before bonding. Specimens without pretreatment were used as negative controls (n = 4 teeth for each group). Microtensile bond strength, failure mode, micromorphologies of resin-dentin interface and the collagen degradation of bonded specimens after thermal cycling were evaluated.</p><p><b>RESULTS</b>After thermal cycling, the microtensile bond strength values of resin-dentin bond in groups pretreated with 15% PA for 120 s and 60 s [(23.09 ± 3.19) and (21.88 ± 3.49) MPa] were significantly higher than that in control group [(15.47 ± 3.78) MPa] (P < 0.05). Mixed fractures were the most prevalent failure mode. Specimens with pretreatment presented compact hybrid layer, while some narrow gaps were found in hybrid layer of non-treated specimens. Collagen biodegradation rates in groups with pretreatment were significantly lower than that in control group (P < 0.05). Among them, specimens pretreated by 15% PA preconditioner for 120 s exhibited the lowest biodegradation rates [(0.316 ± 0.019) mg/g].</p><p><b>CONCLUSIONS</b>The application of natural cross-linker PA on demineralized dentin reduced the bond degradation against aging by thermal cycling, and can be helpful to create more durable bonds to dentin.</p>