Correlation of the contents of trace elements in male body fluids with sperm quality / 中华男科学杂志

<p><b>OBJECTIVE</b>To explore the contents of trace elements in the blood and seminal plasma of men and their correlation with semen parameters.</p><p><b>METHODS</b>We detected and analyzed the contents of calcium, magnesium, copper, zinc, iron and lead in the blood and semen of 113 men with the BH-5100 5-channel atomic absorption spectrometer, YY-1001 blood lead instrument and Weili color sperm automatic analyzer to investigate the correlation of the contents of trace elements with quality.</p><p><b>RESULTS</b>The contents of lead, copper, zinc and iron in the blood were significantly correlated with those in the seminal plasma, but not the contents of calcium and magnesium. The contents of lead, copper, zinc, calcium, magnesium and iron in the blood were significantly different from those in the semen. In the seminal plasma, the content of calcium showed a significant positive correlation with sperm motility, linear motile sperm motility (LMSM), straight line velocity (VSL), curvilinear velocity (VCL), mean angle of deviation (MAD), active sperm density (ASD), average path velocity (VAP) and lateral head amplitude (ALH), while that of lead was negatively correlated with sperm motility, LMSM, ASD, motile sperm density (MSD) and linear motile sperm density (LMSD), and so was that of magnesium with sperm motility, LMSM and LMSD, and those of calcium, magnesium and iron with seminal pH. In the blood, the content of zinc was negatively correlated with sperm motility and LMSM, and so was that of magnesium with sperm motility, LMSM and VAP, while that of lead was positively correlated with ALH.</p><p><b>CONCLUSION</b>The contents of trace elements in different body fluids are differently correlated with sperm quality.</p>

Determination of uric acid in seminal plasma and correlation between seminal uric acid and semen parameters / 中华男科学杂志

<p><b>OBJECTIVE</b>To establish the method of seminal uric acid (UA) determination and investigate the correlation between the seminal UA level and semen parameters.</p><p><b>METHODS</b>The method of seminal UA determination was established by modifying the method of serum UA detection, and its intraassay coefficient of variation (CV) and the difference of the results between different technicians were also observed to evaluate the acceptability of the method. In the meanwhile, the correlations of the seminal UA level with the patients' age, abstinence time, semen volume, pH, sperm concentration, motility, the percentage of grade a and b sperm, and the percentage of morphologically normal sperm.</p><p><b>RESULTS</b>The intraassay CV was 9. 16% for the method of seminal UA detection, and there was no significant difference in the UA level detected by 2 technicians (P = 0.541). The seminal UA level was positively correlated with the percentage of morphologically normal sperm (r = 0.350, P = 0.025) , with a tendency of positive correlation with sperm motility (r = 0.147, P = 0.085) and the percentage of grade a and b sperm (r = 0.156, P = 0.068), but not with other parameters such as semen volume, pH, sperm concentration, abstinence time and the patients' age.</p><p><b>CONCLUSION</b>An acceptable method of seminal UA determination could be established by modifying the method of serum UA detection. Sperm morphology, motility and the percentage of grade a and b sperm might be related to the level of seminal UA.</p>