Melatonin inhibiting the migration and invasion of gastric cancer cell line SGC-7901 / 解剖学报

Objective To investigate the inhibitory effect of melatonin on the migration and invasion of gastric cancer cell and the underlying molecular mechanism. Methods Human gastric cancer SGC-7901 cells were treated with different concentrations of melatonin ( 0. 1, 1, 2 and 4 mmol/L ) for 24 hours, and the changes in the migration and invasion of gastric cancer cells were detected by scratch test and Transwell assay. The expressions of matrix metalloproteinase ( MMP) -2 and MMP-9 in the supernatant were detected by ELISA kit. The changes of MMP-2, MMP-9, intercellular cell adhesion molecule-1 ( ICAM-1 ) and CD44 expressions were detected by using Real-time PCR. The protein expressions of ICAM-1, CD44, p-P38, P38 and phosphorylated mitogen-activated protein kinase kinase ( p-MKK) 3/6 were detected by Western blotting. Results Melatonin inhibited the migration and invasion of gastric cancer cells in a dose- dependent manner. Compared with the blank control group, melatonin reduced the expression of MMP-2, MMP-9, ICAM-1 and CD44, and inhibited the expressions of p-P38, P38 and p-MKK3/6 in gastric cancer cells. Conclusion Melatonin inhibits the migration and invasion of gastric cancer cells by inhibiting the expressions of MMP-2, MMP-9, ICAM-1 and CD44. Inhibition may be related to the p38MAPK signaling pathway.

Anti-gastric cancer effect of melatonin and Bcl-2, Bax, p21 and p53 expression changes / 生理学报

In order to investigate the role of melatonin in inhibiting the proliferation of murine gastric cancer and the underlying molecular mechanism, we performed an in vivo study by inoculating murine foregastric carcinoma (MFC) cells in mice, and then tumor-bearing mice were treated with different concentrations of melatonin (i.p.). The changes of Bcl-2, Bax, p21 and p53 expressions in tumor tissue were detected by using real-time fluorescence quantitative RT-PCR and Western blot. We found that: (1) melatonin resulted in reductions of tumor's volume and weight in the gastric cancer-bearing mice and thus showed anti-cancer effect; (2) melatonin reduced Bcl-2 expression, but increased the expression of Bax, p53 and p21 in tumor tissue. Our results suggest that melatonin could inhibit the growth of tumors in gastric cancer-bearing mice through accelerating the apoptosis of tumor cells.

Involvement of protein kinase C in NMDAR-dependent long-term potentiation in rat amygdala / 生理学报

The mechanism of long-term potentiation (LTP) in basolateral amygdala (BLA) was explored using field potential recording in rat brain slice preparation. Field potentials (field excitatory post-synaptic potentials, fEPSPs) in BLA were evoked with sharpened steel bipolar stimulating electrodes placed in the external capsule (EC). Two theta burst stimulations (TBS, interval=10 min) induced LTP in BLA. TBS-induced synaptic potentiation lasted for more than 30 min after the second TBS. LTP in BLA was input-specific and was blocked by N-methyl-D-aspartate receptor (NMDAR) antagonist 2-amino-5-phosphonovaleric acid (APV). The effect of protein kinase C (PKC) on LTP was then determined using PKC inhibitor chelerythrine chloride. Bath application of chelerythringe chloride had no effect on basic field potentials and paired-pulse ratio (PPR). However, in the presence of chelerythrine chloride, two TBS failed to induce LTP. In contrast, bath application of chelerythrine chloride 10 min after the second TBS did not affect the maintenance of LTP in BLA. These results indicate that LTP is NMDAR-dependent and PKC is involved in the induction and early maintenance of LTP in BLA.

Effect of jiangu granule on levels of urinary deoxypyridinoline and osseous intigrin beta1 mRNA expression in Ovariectomized osteoporosis model rats / 中国中西医结合杂志

<p><b>OBJECTIVE</b>To investigate the regulatory effect of Jiangu Granule (JGG) on osteoblast and its effect on bone absorption on the molecular level and from aspect of bone metabolism.</p><p><b>METHODS</b>Osteoporosis model SD rats established by ovariectomy were interfered by drug. Levels of urinary deoxypyridinoline (D-Pyr) and osseous integrin beta1 (Itgbeta1) mRNA expression were detected by ELISA and in situ hybridization, respectively.</p><p><b>RESULTS</b>Level of urinary D-Pyr increased (P < 0.01) and osseous Itgbeta1 mRNA expression decreased significantly in the model rats. After treatment with JGG for 12 weeks, the D-Pyr levels in them decreased significantly (P < 0.05), while the quantity of osteoblasts and the expression of Itgbeta1 mRNA in bone tissue increased obviously. Conclusion JGG can not only decrease the catabolism of collagen fiber type I in bone matrix, but also promote the osteogenic activity of osteoblast. So, it can improve or reverse the pathological tendency of osteogenesis shortage caused by E2 reduction to play its action in strengthening bone.</p>

Effect of jiangu granule on quality of bone in model rats with osteoporosis induced by ovariectomy / 中国中西医结合杂志

<p><b>OBJECTIVE</b>To study the effect of Jiangu granule (JGG) on quality of bone in model rats with osteoporosis.</p><p><b>METHODS</b>Osteoporosis model was established by means of ovariectomy. Bone mineral density (BMD) was measured with dual energy X-ray densitometry. Osseous tissue structure of upper tibia was observed by ono-decalcified section and toluidine blue staining, and morphometry was carried out. Compressive strength limit and elastic modulus of first lumbar vertebrae, and bend load of femur were measured by light-based elastometer.</p><p><b>RESULTS</b>JGG can significantly increase BMD, area of trabecula and thickness of bone cortex/diameter of marrow cavity ratio of model rats, to elevate the compressive strength limit of vertebrae and bend load of femur.</p><p><b>CONCLUSION</b>JGG could increase BMD of osteoporosis model rats, and also could effectively improve the structure of osseous tissue, biomechanical property of bone and enhance the overall quality of bone.</p>

Treating the urinary incontinence in stroke patients with acupuncture,moxibustion and physical therapy / 中华物理医学与康复杂志

Objective To analyze the urodynamic abnormalities of incontinent stroke patients,and evaluate the effects of acupuncture,moxibustion in combination with physical therapy.Methods One hundred and six in- continent stroke patients were divided into a control group and a treatment group.The control group was treated with conventional methods,and the treatment group with acupuncture,moxibustion and physical therapy modalities such as ultrasound and EMG biofeedback.The Urodynamic examination was performed before and after treatment.Re- sults It was found that the uradynamic abnormalities in the incontinent stroke patients include detrusor hyperreflexia and uninhibited sphincter relaxation.Combined use of acupuncture,moxibustion and physical therapy can significant- ly improve the patients' symptoms.Conclusion Common urodynamic abnormalities.are detrusor hyperreflexia and uninhibited external sphincter relaxation in incontinent stroke patients.Combined use of acupuncture,moxibustion and physical therapy is effective for treating this condition.