Vitrectomy combined with heavy silicone oil tamponaded for severe proliferative vitreous retinopathy / 国际眼科杂志(Guoji Yanke Zazhi)

@#AIM: To assess clinical efficacy of vitrectomy combined with heavy silicone oil tamponaded for severe proliferative vitreous retinopathy. <p>METHODS: Totally 13 severe proliferative vitreous retinopathy(PVR)patients(13 eyes)admitted to our hospital between June 2012 and December 2015 were included. We analyzed the clinical efficacy of vitrectomy combined with heavy silicone oil tamponaded and heavy silicone oil removal combined with C₃F₈ tamponaded at late phase for 13 patients. Heavy silicone oil removal of 13 patients were performed at 10 to 17wk after heavy silicone oil tamponaded. The patients were reviewed at 1-7d after vitrectomy combined with heavy silicone oil tamponaded, 1, 2, 4 and 17wk after hospital discharge, 1-7d after the heavy silicone oil removal, 1, 2, 4, 8, 12 and 24wk after the second hospital discharge. Patients were followed up for at least 24wk after the heavy silicone oil removal. The observed indicators included the rate of retinal reattachment, the best corrected visual acuity, intraocular pressure(IOP), intraocular lens and postoperative complications.<p>RESULTS: During follow-up,13 eyes showed a stably reattached after heavy silicone oil tamponaded. Heavy Silicone oil of 13 patients were removed combined with C₃F₈ tamponaded for 10 to 17 wk after heavy silicone oil tamponaded. But the fifth case had retinal detachment recurrence for 4wk after heavy silicone oil removed because of macular hole and the eighth case had retinal detachment recurrence for 8wk after heavy silicone oil removed because of new superior temporal retinal hole. Follow up for 24wk, the other 11 eyes showed a stably reattached after heavy oil silicone removed. The best corrected visual acuity range of 13 eyes were in light perception to hand move before heavy oil silicone tamponaded and the best corrected visual acuity range of 13 eyes were in hand move to 20/250 for 24wk after heavy oil silicone removed. The best corrected visual acuity of the fifth case and the eighth case were count finger and hand move for 24wk after heavy oil silicone removed. Four eyes had high intraocular pressure 1wk after heavy oil silicone tamponaded which were reduced to 10-21mmHg after drug treatment. At late phase 5 eyes had drug uncontrollable high intraocular pressure which we took silicone oil removal timely. Three eyes had temporarily high intraocular pressure after heavy oil silicone removed which were reduced to 10-21mmHg after drug treatment. At late phase 3 eyes did not appear high intraocular pressure after stopping anti-glaucoma medication. Thirteen eyes had no serious complications such as anterior chamber inflammation and endophthalmitis during the treatment. <p>CONCLUSION: Vitrectomy combined with heavy silicone oil tamponaded and heavy oil silicone removed combined with C₃F₈ tamponaded at late phase for 13 severe proliferative vitreous retinopathy patients could achieve a satisfactory rate of retinal reattachment and improve the patients' prognostic visual acuity.

Implication of MAPK in sodium salicylate-induced heat shock protein 27 expression in human lens eplthelial Cells in vitro / 中华实验眼科杂志

Background Heat shock proteins (HSPs) are highly conserved proteins that are induced in cells when confronted with a wide variety of proteotoxic stresses.HSP27 has a high degree of similarity with α-crystallin protein.The abnormality of HSP27 structure and expression are closely related to the formation of cataracts.Our previous study showed sodium salieylate has the protective effect on H2O2-induced lens damage.Objective This study was to investigate the roles of MAPK signal pathway in sodium salicylate-induced the expression of HSP27 in human lens epithelial cells (LECs) in vitro.Methods Human LECs were incubated in the fresh media containing sodium salicylate at different concentrations (0-55 mmol/L) for different times (1-5 hours) and allowed to be recovered in fresh medium without sodium salicylate for 1-24 hours with or without pretreatment with P38MAPK inhibitor (SB203580), ERK1/2 inhibitor (PD98059) and JNK/SAPK inhibitor ( SP600125). The expressions of P38MAPK, EBK1/2, JNK/SAPK, phosphorylated P38MAPK, phosphorylated ERK1/2, phosphorylated JNK/SAPK and HSP27 were detected by Western blot. HSP27 mRNA was detected by RT-PCR. The expression of HSP27 was also detected by immunohistochemistry. Results There was only weak expression of HSP27 in normal human LECs.After stimulation of 35-55 mmol/L sodium salicylate was removed and human LECs were cultured again for 6 hours,the expression of HSP27 in LECs were significantly increased ( F= 509. 953,P<0. 01). HSP27 was absent expressed in human LECs in 55 mmol/L sodium salicylate stimulation for 1-5 hours groups, but LECs were re-cultured for 3,6 hours after removed the stimulation, the expression of HSP27 was elevated (F = 452. 534, P<0. 01). Activation of P38 M APK occurred after sodium salicylate stimulation 30 minutes and 1 hour ( F = 865.68, P<0. 01). However, ERK 1/2 was expressed after sodium salicylate was eliminated for 1-6 hours ( F = 388.84, P<0. 01). JNK/SAPK was inactived by sodium salicylate. The expression of HSP27 could be down-regulated with the pretreatment of SB203580 and PD98059. Conclusion Sodium salicylatc can induce the expression of HSP27 in human (LECs) . The effects are mediated,at least in part ,through the activation of P38MAPK and ERK1/2 signaling pathway .