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1.
Journal of Zhejiang University. Science. B ; (12): 39-44, 2007.
Artículo en Inglés | WPRIM | ID: wpr-309038

RESUMEN

Forkhead box (Fox) proteins play critical roles in the regulation of differentiation, proliferation, immunity and aging of cells. Most studies on Fox proteins are limited to cultured cells and rodent. The aim of the current study is to detect by immunohistrochemistry whether FoxO1, FoxO3a and FoxO4 proteins are localized in the stomach and intestine of the pig. The results showed that FoxO4 exists in the mucosa in all parts of the stomach and intestine; FoxO3a exists mainly in the lamina propria and muscularis of some parts. However, FoxO1 is not detectable in all parts of the stomach and intestine. Collectively, the results of the present study indicate that there exists a distinct expression pattern of Fox proteins, and that FoxO4 is a primary forkhead transcriptional factor localized in the gastrointestinal tracts of the pig.


Asunto(s)
Animales , Femenino , Masculino , Factores de Transcripción Forkhead , Metabolismo , Tracto Gastrointestinal , Metabolismo , Inmunohistoquímica , Sus scrofa , Metabolismo , Distribución Tisular
2.
China Journal of Chinese Materia Medica ; (24): 107-110, 2005.
Artículo en Chino | WPRIM | ID: wpr-276637

RESUMEN

<p><b>OBJECTIVE</b>To study, the high polar media-water extraction process of Herba epimedii with the microwave technology.</p><p><b>METHOD</b>The single parameters of extraction process was investigated with dry extract yield and extraction efficiency of icariin as content index.</p><p><b>RESULT</b>Through studying the single parameter, the optimal conditions are as follows: 70% ethanol as wetting solvent, volume of solvent to weight of material equal to 6:1 in wetting, 30 min wetting time, 2.5 min microwave irradiation time, volume of solvent to weight of material equal to 50:1 in extraction process, 10 min microwave-assisted extraction time.</p><p><b>CONCLUSION</b>Compared with conventional methods, microwave-assisted extraction (MAE) after Microwave Pretreament (MP) of raw material was higher extraction efficiency and time-saving.</p>


Asunto(s)
Medicamentos Herbarios Chinos , Epimedium , Química , Flavonoides , Microondas , Plantas Medicinales , Química , Solventes , Tecnología Farmacéutica , Métodos
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