Effect of macrophages on the differentiation of mouse induced pluripotent stem cells into hepatic progenitor cells / 临床肝胆病杂志

ObjectiveTo investigate the effect of macrophages (MCs) on the differentiation of mouse induced pluripotent stem cells (iPSCs) into hepatic progenitor cells (HPCs). MethodsA total of 24 C57BL/6N mice were used to obtain MCs by peritoneal irrigation, and the supernatant was collected to obtain the conditioned medium of MCs (MC-CDM). Activin A, bone morphogenetic protein 4, and fibroblast growth factor were used to induce the differentiation of mouse iPSCs into HPCs. The differentiation of HPCs were randomly divided into control group (normal medium) and experimental group (MC group; use of MC-CDM medium on day 5 of induction). Morphology, immunofluorescence assay, and Western blot were used to compare the morphology of HPCs and the expression of related proteins between the control group and the MC group. The t-test was used for comparison of continuous data between two groups. ResultsHPCs derived from iPSCs were established in vitro, and HPCs had the potential to differentiate into hepatocytes. Immunofluorescence assay showed that compared with the D12 control group, the D12 MC group had a significant increase in the protein expression of the HPC-specific protein CK19 (0.901±0.072 vs 0.686±0.097, t=-3.093, P＜0.05). Western blot showed that compared with the D12 control group, the D12 MC group had a significant increase in the protein expression of the HPC-related protein CK19 (1.922±0.103 vs 1.448±0.012, t =-7.881, P ＜005), as well as a significant increase in the protein expression of the autophagy-related protein LC3 (1.392±0.042 vs 1.101±0048, t =-5.978, P＜005). ConclusionMCs can promote the differentiation of mouse iPSCs into HPCs, possibly by increasing the autophagy level of HPCs.

Catheter Associated Infection and Preventive Measures in ICU / 中华医院感染学杂志

OBJECTIVE To study the incidence of catheter associated infection,bacterial infections and pathogens distribution in ICU,so as to offer a reference to the prevention and control of hospital infection.METHODS The patients with catheter were investigated and specimens were underwent a process of germ culture and identification from ICU in 2006 to a first half year of 2008.RESULTS The incidence of hospital infection was 21.54‰ in ICU.The incidence of ventilator associated pneumonia(VAP) was 23.14‰.The incidence of urinary tract catheter infection was 10.31‰.The incidence of vascular associated infection was 2.74‰.There was a significant difference between the incidence of catheter associated infections and that of no catheter hospital infection(P