Bone regeneration and graft material resorption in extraction sockets grafted with bioactive silica-calcium phosphate composite versus non-grafted sockets: clinical, radiographic, and histological findings

Purpose@#The purpose of the present study was to evaluate the effect of silica-calcium phosphate composite (SCPC) granules on bone regeneration in extraction sockets. @*Methods@#Ten patients were selected for a split-model study. In each patient, bone healing in SCPC-grafted and control ungrafted sockets was analyzed through clinical, radiographic, histomorphometric, and immunohistochemical assessments 6 months postoperatively. @*Results@#A radiographic assessment using cone-beam computed tomography showed minimal ridge dimension changes in SCPC-grafted sockets, with 0.39 mm and 1.79 mm decreases in height and width, respectively. Core bone biopsy samples were obtained 6 months post-extraction during implant placement and analyzed. The average percent areas occupied by mature bone, woven bone, and remnant particles in the SCPC-grafted sockets were 41.3%±12%, 20.1%±9.5%, and 5.3%±4.4%, respectively. The percent areas of mature bone and woven bone formed in the control ungrafted sockets at the same time point were 31%±14% and 24.1%±9.4%, respectively. Histochemical and immunohistochemical analyses showed dense mineralized bundles of type I collagen with high osteopontin expression intensity in the grafted sockets. The newly formed bone was well vascularized, with numerous active osteoblasts, Haversian systems, and osteocytes indicating maturation.In contrast, the new bone in the control ungrafted sockets was immature, rich in type III collagen, and had a low osteocyte density. @*Conclusions@#The resorption of SCPC granules in 6 months was coordinated with better new bone formation than was observed in untreated sockets. SCPC is a resorbable bone graft material that enhances bone formation and maturation through its stimulatory effect on bone cell function.Trial Registration: ClinicalTrials.gov Identifier: NCT03897010

Bone regeneration and graft material resorption in extraction sockets grafted with bioactive silica-calcium phosphate composite versus non-grafted sockets: clinical, radiographic, and histological findings

Purpose@#The purpose of the present study was to evaluate the effect of silica-calcium phosphate composite (SCPC) granules on bone regeneration in extraction sockets. @*Methods@#Ten patients were selected for a split-model study. In each patient, bone healing in SCPC-grafted and control ungrafted sockets was analyzed through clinical, radiographic, histomorphometric, and immunohistochemical assessments 6 months postoperatively. @*Results@#A radiographic assessment using cone-beam computed tomography showed minimal ridge dimension changes in SCPC-grafted sockets, with 0.39 mm and 1.79 mm decreases in height and width, respectively. Core bone biopsy samples were obtained 6 months post-extraction during implant placement and analyzed. The average percent areas occupied by mature bone, woven bone, and remnant particles in the SCPC-grafted sockets were 41.3%±12%, 20.1%±9.5%, and 5.3%±4.4%, respectively. The percent areas of mature bone and woven bone formed in the control ungrafted sockets at the same time point were 31%±14% and 24.1%±9.4%, respectively. Histochemical and immunohistochemical analyses showed dense mineralized bundles of type I collagen with high osteopontin expression intensity in the grafted sockets. The newly formed bone was well vascularized, with numerous active osteoblasts, Haversian systems, and osteocytes indicating maturation.In contrast, the new bone in the control ungrafted sockets was immature, rich in type III collagen, and had a low osteocyte density. @*Conclusions@#The resorption of SCPC granules in 6 months was coordinated with better new bone formation than was observed in untreated sockets. SCPC is a resorbable bone graft material that enhances bone formation and maturation through its stimulatory effect on bone cell function.Trial Registration: ClinicalTrials.gov Identifier: NCT03897010

Evaluation of preservative capacity for some selected cosmetic products found in the market

Aim of the study: To determine the preservative capacity of different cosmetic preparations commonly found in the Egyptian markets

Methods: Microbiological evaluation of 74 cosmetic sample and preservative capacity test for cosmetic samples showing no microbial contamination using rejecting microorganisms by cup plate technique

Results: It was found that 29 samples were contaminated at levels >103 or >500 [for baby care products] or contaminated with rejecting microorganisms or both. The preservative capacity was variable between the different types and brands of the tested cosmetics against bacteria [P<0.05], while for Candida albicans all samples of the different cosmetic types were of nearly the same effect [p>0.05]

Conclusion: The detection of microbial counts greater than the microbial limits standards and isolation of rejecting microorganisms are clear evidences of non-adherence to good Manufacturing Practices. Variable preservative capacity in some cosmetics may indicate its ability to withstand microbial contamination which leads to spoilage of these cosmetics

Sentinel surveillance for patients with acute hepatitis in Egypt, 2001-04

Viral hepatitis is a major problem in Egypt. To define the epidemiology of the disease, sentinel surveillance was established in 5 hospitals in diverse areas of the country in 2001. Data were completed for patients meeting the case definition for viral hepatitis. Of a total of 5909 patients evaluated, 4189 [70.9%] showed positive antibody markers for hepatitis. Out of those, 40.2% had evidence of hepatitis A virus [HAV] infection, 30.0% hepatitis B virus [HBV] and 29.8% hepatitis C virus [HCV] infection. This surveillance system was useful in identifying the variable endemicity of acute HAV infection in different regions and for better understanding the epidemiology of HBV and HCV infection

Ruptured sinus of valsalva aneurysm initially misdiagnosed as ventricular septal defect by echocardiography

Aneurysms of sinus of valsalva are rare cardiac lesions and most of them are in congenital origin. The malformation consists of a separation or lack of fusion between the media of the aorta and the annulus fibrosis of the aortic valve. The structure becomes aneurysmal and may rupture to form a fistula. We present a case of ruptured sinus of valasalva aneurysm in a 25-year-old man. The diagnosis was made by echocardiography and confirmed at operation

pharmacognostical study of Monsonia nivea decne. WEBB. growing in Egypt

The macro- and micro-morphological characters of the leaves, petioles, stems and roots of Monsonia nivea Decne. Webb. [Family Geraniaceae] have been studied in order to identify them in both entire and powdered forms

Phytochemical and biological investigation of Monsonia Nivea decne.webb. growing in Egypt

Six compounds were isolated from Monsonia nivea Decne. Webb. [Geraniaceae] growing in Egypt. The compounds are sterol, triterpene and flavonoid in nature. These compounds were identified as alpha-amyrin, beta-sitosterol, ursolic acid, apigenin-7-O-beta-D-glucopyranoside, quercetin-3-O-glucoside and rutin. The structures of the isolated compounds were confirmed by different spectroscopic methods. The anti-oxidant, anti-bacterial and the DNA-binding activities [as an indicator for anti-tumor activity] were carried out for the extracts and some pure compounds

Rapid detection of Candida species in intensive care unite of neonates and premature infants by species specific PCR of V3 region of large subunit ribosomal DNA

An increasing incidence of systemic candidiasis has been reported in neonates and low birth weight infants requiring intensive care. The rapid detection and identification of Candida species in clinical laboratories are extremely important for the management of patients with hematogenous candidiasis. The presently available culture and biochemical methods for detection and species identification of Candida are time consuming and lack the required sensitivity and specificity. In this study, we have applied nested PCR [nPCR] using universal and species-specific primers for detection of Candida species in clinical specimens. Primers to conserved sequences in the V3 region of large subunit ribosomal DNA [rDNA] were used to amplify DNA from Candida species, including, C. albicans, C. krusei, C. glabrata, and C. parapsilosis. Candida isolates showed 99% concordant results with CHROMagar Candida identification system. Evaluation by nPCR for detection of Candida species in, suspected [n ' 16] patients and healthy subjects [n ' 14] showed that nPCR results were consistently negative in healthy subjects. In the category of proven candidemia with negative blood cultures for Candida, seven patients [43.75%] were positive by nPCR; two of them had dual infection with C. albicans and either C. parapsilosis or C. glabrata. In conclusion, the nPCR developed in this study is specific and more sensitive than culture for the detection of Candida species in clinical specimens

Phytochemical and biological investigation of leptadenia pyrotechnica dence growing in Egypt

The phytochemical investigation of Leptadenia pyrotechnica Dence [Family Asclepiadaceae] growing in Egypt was performed in this study. Sterols and triterpenes were isolated; namely, beta-amyrin, beta-sitosterol, lupeol and betulin. In addition, five flavonoid compounds, viz. quercetin, kaempferol-3-O-beta-D-glucoside, isorhamnetin-3-O-rutinoside, quercetin-3'- O-beta-D-glucoside and rutin. The isolation and identification of these compounds were achieved through different chromatographic and spectroscopic methods. The isolated pure compounds were subjected to a DNA-binding assay to reveal any antitumor activity of these compounds. Only betulin showed a moderate DNA-binding activity at IC50, 42 mug/ml

Antimicrobial flavonoids from solanum dobium fres

Two flavonoids compounds were isolated from Solanum dobium Fers [Solanaceae]. One major compound was identified as astragalin [kampferol-3-O-glucoside], and a minor compound that was tentatively identified as quercetin-3-glycoside. The antimicrobial activities of the 2 compounds were tested against 12 microorganisms [7 bacteria, 2 fungi and 3 C and ida of different strains]. The major compound was found to possess a pronounced antibacterial activity as presented by the minimum inhibitory concentration showing activity 4 times as that of streptomycin and double that of tobramycin against Pseudomonas aeruginosa UM 60690. It displayed double activity of streptomycin against both Salmonella typhi UM 26049 and Flavobacterium meningosepticum UM 260494. In addition, the isolated astragalin showed a significant 2-4 times antic and idal activity as compared with nystatin against C and ida intermedia ATCC 5159, C and ida albicans Um 050494, and C and ida lipolytica ATCC 8661. However, this compound did not show any significant antifungal activity. The isolated minor flavonoid showed significant antic and idal, antifungal as well as moderate antibacterial activity

comparative macro- and micromorphology of the leaves, stems and stems and seeds of Vicia sative 1, Vicia calcarate desf and Vicia faba L, [var Minor] growing in Egypt

A macro- and micromorphology of the leaves, stems and seeds of Vicia sativa L., V. calcarata Desf, and V. faba L. [var. Minor] growing in Egypt are presented for the sake of their identification and differentiation in both entire and powdered forms

Macro and micromorphology of leaves, stems and roots of plantago psyllium L growing in Egypt

A macro- and micromorphology of the leaves, stems and roots of Plantago psyllium L. growing in Egypt are presented

Macro and micromorphology of ipomoea carnea jaq growing in Egypt part III: fruit and seed

A study of the macro- and micromorphological characters of the fruit and seed of Ipomoea carnea Jacq. is given in both entire and powdered forms. Many plants belonging to the family Convolvulaceae are known to be useful as purgative drugs. Ipomoea carnea Jacq. is perennial, erect shrub, indigenous to Belgium and was introduced to Egypt for ornamental purpose. Nothing was reported in the current literature concerning both chemical and botanical studies of the plant. The chemical investigation of the resin contents of the plant was recently reported. Meanwhile, the botanical studies of the leaves and flowers, stem and roots were reported, so it was found to be interesting to investigate the botanical characters of the fruits and seeds

Impact of hepatitis C virus [HCV] seropositivity on the serum protein electrophoretic pattern

The impact of HCV seropositivity on the serum protein electrophoretic pattern was analyzed. Abnormalities were tested for prediction of hepatic dysfunction. Sera were obtained from 20 asymptomatic patients seropositive for the virus and 20 control subjects. Sera were subjected to further testing of HCV seropositivity using the second generation enzyme immunonassay kit, and the pattern of binding of anti-HCV antibodies to HCV structural [C1 and C2] and non structural [NS3 and NS4] antigens was determined using the immunoblotting technique. The serum protein electrophoretic pattern was determined and the levels of the different fractions were tested for correlation to the levels of the liver enzymes, serum level of immunoglobulins and to the pattern of reactivity to structural and non structural HCV antigents. The results suggested the importance of the hypergammaglobulinemia as a marker of hepatic dysfunction in HCV seropositive patients, its level might be of value in the follow up of patients with normal liver transaminases levels

T-Cell phenotypes and PHA blastogenic response in symptomatic and asymptomatic Giardia SIS

To determine the relation and the possible significance of peripheral blood T-cell subsets and PHA blastogenesis to various clinical manifestations of Giardiasis, 20 symptomatic, 27 asymptomatic and 18 apparently healthy individuals of matched age and sex were included in this study. Functional analysis of peripheral blood mononuclear cells of asymptomatic Giardia lambia-infection as measured by 3H-thymidine uptake in response to PHA mitogen was significantly inhibited as compared to symptomatic cases of Giardiasis and reference subject groups. On the other hand, phenotypic analysis of the T-lymphocyte subsets showed that CD4+, CD8+, as well as CD4+/CD8+ ratio of asymptomatic, symptomtatic Giardiasis and control groups to be statistically not different from each other. The results suggested a possible role of cell mediated immunity in clearing Giardia lamblia- infection, i.e. development of asymtomatic cases. Meanwhile, the results of the present work showed a defective correlation between peripheral T-lymphocyte subsets and clinical manifestations, i.e. asymptomatic Giardiasis

Phenylpropanoid glycosides from orobanche aegyptiaca and orobanche ramosa

Four phenyl propanoid glycosides, acteoside, 2'-0-acetyl acteoside, poliumoside and 2'-0-acetyl poliumoside were isolated from the butanol extract of Orobanche aegyptiaca Pers. and poliumoside was isolated from the butanol extract of O. ramosa L. The chemotaxonomy of these species was discussed

Essential oils of Thuja occidentalis, Thuja orientalis, Cupressus sempervirens and Juniperus phoenicea

Essential oils were obtained by steam distillation from the twigs and berries of Thuja occidentalis [0.25 and 0.5% w/w respectively], Thuja orientalis [0.32 and 0.25%,w/w], Cupressus sempervirens [0.4 and 0.16 w/w], and Juniperus phoenicea [0.75 and 1.25% w/w]. Comparisons of the four essential oils were made by gas chromatography/mass spectral [GC/MS] analysis. Major constituents of the oils, alpha and were alpha-thujene, [+] sabinene B-pinenes, [+]-camphene, cedrol and cedrenol

Some phenylpropanoid glycosides from Orobanche crenata forsook

A new glycoside, decaffeoylcrenatoside and a known phenylpropanoid glycoside, cistanoside F, have been isolated from the butanol extract of Orobanche crenata Forssk. Crenatoside and acteoside have been identified by TLC. The structures of descaffeoylcrenatoside as well as the known one cistanoside F were established through chemical and spectroscopical methods

Investigation of the lipid and flavonoid content of some orobanche species

Chemical investigation of the petroleum ether and ether extracts of Orobanche aegyptiaca Pers., O. crenata Frossk. and O. ramosa L. afforded a hydrocarbon, a mixture of beta sitosterol, stigmasterol and compesterol, beta sitosterol-3-D-glucoside, tricin, apigenin and luteolin. The fatty acid composition of these plants have been investigated. The chemotaxonomic value of the chemical composition of these extracts and the fatty acid composition have been discussed

Macro-and micromorphology of Veronica anagallis-aquatic var, anagalloides schinzethel

The macro-and micromorphological characters of the different parts of veronica anagallis-aquatica var. anagalloides Schinz et Thell. viz; leaf, stem, flower and root are presented aiming for their identification in both the entire and powdered forms