Some biological and genetic studies on rats treated with acrylamide with or without nigellla sativa with special reference to their effects on glutathione-s-transferase and reduced glutathione

The present study was conducted to explore the effects of acrylamide [ACR] and Nigellia sativa [N.S] [which was used as a natural protection] on biochemical, hematological and genetic profiles of Albino rats. The obtained data showed that the lowest values of serum enzymatic activities [Asparatate and Alanine amino transferase [AST, ALT]] Gamma glutamyl transferase, [GGT] and Lactate dehydrogenase [LDH] were noticed in sera of rats treated with [ACR] followed by those given [N.S] with [ACR]. Furthermore, the glutathione-S-transferase and reduced glutathione [GSH] values were also significantly reduced in liver and kidneys of both groups treated with [ACR] after one and two weeks. Insignificant increase in activities of all studied enzymes as well as reduced glutathione [GSH] was recorded in rats treated by Nigella sativa only. On the other hand, the lowest values of packed cell volume [PCV], hoemoglobin [Hb], RBCs, WBCs, acidophils and lymphocytes were observed in [ACR] treated group allover the experimental period, these results nearly reversed in rats treated with Nigella sativa only. A slight improvement in biochemical and hematological criteria was observed in rats given [ACR] plus [N.S]. The obtained results revealed a significant decrease in the rate of mitosis in [ACR] treated group during the experimental period. The chromosomal structure and number is significantly altered in both groups treated with Acrylamide. Finally, the microscopic and ultra structural changes were observed only in the 14[th] day of experiment especially in rats treated with [ACR] and [ACR] plus [N.S]. Furtherly encountered lesions were more severe in [ACR] treated group than those given [ACR] plus [NS]

Study of the effect of high dietary iodine on blood of broiler chickens

The aim of this study was to elucidate the effect of high dietary iodine on some biochemical and hematological parameters of broiler chickens. The obtained data showed that the thyroidal hormones concentration [T3 and T4], alkaline phosphatase activity [ALP], aspartate aminotransferase activity [ASAT] and urea level were significantly increased in the sera of chickens kept on high dietary iodine [150 ppm in drinking water] at the 3rd and the 6th weeks post treatment. On the other h and, calcium, iron and creatinine levels were significantly increased [P <0.05] at the 6th week only. Concerning the blood picture, the obtained results revealed that, the red blood cells [RBCs] count, lymphocytic percentage, hemoglobin [Hb] concentration and packed cell volume [PCV] values were significantly increased in the iodine supplemented chickens, while the white blood cells [WBCs] count and heterophil percentage were significantly decreased in the same group

Effect of dietary cholesterol and olive oil on serum lipids and yolk cholesterol of laying hens

Effect of dietary cholesterol and olive oil on serum lipids and egg yolk cholesterol were investigated. Thirty Hisex brown hens at 25 weeks of age were used in two trials by feeding two basal diets containing 1% cholesterol with or without 10% olive oil. The feeding of cholesterol alone resulted in a significant decrease in serum phospholipids, while cholesterol-olive diet increased its level. In contrast. feeding of cholesterol alone caused an increase in the serum levels of triacylglycerol, total lipids and cholesterol. The previous results were adversed by adding olive oil to cholesterol in the diet. Although, adding of olive oil to diet containing cholesterol leads to a decease in the serum cholesterol level, yet it fails to make the same effect in the egg yolk cholesterol content