RESUMEN
Glutamine plays a key role in cell protection following stress, by causing a simultaneous increase in hsp72 expression, which is dependent on glutamine metabolism and its relation with cortisol levels and gluconeogenesis. To assess the effect of glutamine supplement ingestion on hsp72, cortisol and glucose plasma changes after exercise, 28 soccer players were divided in four groups; the control, supplement, supplement-exercise and the exercise groups.0.5 g/kgBw supplement and placebo consumed 5 ml/kgBw volume of water one hour prior to the exercise protocol, which included 3 stages of 20 minutes running [80% HRmax intensity] with 5 minute rest periods between each stage. Baseline, and pre, post and 90 minutes after exercise, blood sampling was done and cortisol, glucose and hsp72 levels were measured using RIA, Enzymatic, and Elisa tests respectively. Data was analyzed with MANOVA and Bonferoni post hoc tests, P
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Backgroun and aims: Anterior knee pain is a common problem among adults and young people. This study aimed to investigate the effects of low-level LASER on improvement of knee pain and function in patients with anterior knee pain
Methods: This double-blind, randomized clinical trial was carried out in Zahedan University of Medical Sciences, in 2008. In this study, thirty patients were randomly divided into experimental [N=15] and control groups [N=15]. In the experimental group, beside the exercise, a low-level Ga-As LASER was applied with a 100 mW average power, wavelength 905 nm and 8 J/cm2 dosages for 3 minutes duration. In the control group, sham LASER was used. A 16 session treatment program [during 4 weeks, 4 sessions per weeks] was performed for both groups. Before and after the intervention, we measured the pain level through visual analog scale [VAS] and the function by Knee and Osteoarthritis Outcome Score [KOOS] and range of knee flexion by goniometry. Paired and Independent t-tests were used for data analysis
Results: The knee pain decreased from 6.06+/-2.6 to 2.7+/-2.6 in the experimental group [P<0.001] and from 6.4+/-1.9 to 3.4+/-1.4 in the control group [P=0.003]. KOOS score was decreased from 87.2+/-29.8 to 48.4+/-24.1 in the experimental group and from 94.8+/-20.9 to 61.7+/-15.3 in the control group [P<0.001]. Level of pain was significantly decreased in the experimental group compared to the control group [P=0.012]
Conclusion: The results showed that exercise therapy with or without LASER can reduce pain and cause better knee function and expansion of the ranges of knee flexion. However; LASER is more effective in decreasing anterior knee pain
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The aim of this study was to determine the frequency of enterotoxigenic and enteroaggregative strains of Escherichia coli in chicken carcasses by polymerase chain reaction [PCR]. In this study 63 strains of E. coli were isolated from 110 samples of chicken carcasses during processing after chilling in the poultry slaughter house of Shahrekord. Polymerase chain reaction assays were used to detect the presence of the genes encoding heat-stable enterotoxin a [STa], heat-stable enterotoxin b [STb], heat labile toxin [LT] and Enteroaggregative heat-stable toxin 1 [EAST1]. Sixty three out of 110 [57.27%] carcasses were contaminated with E. coli. Six out of 63 [9.52%] harbored the gene for LT, 1 [1.58%] STb, 21 [33.3%] EAST1 and 8 [12.69%] contain both LT and EAST1 genes. None of the strains contain the STa gene. The results indicated that contamination of the chicken carcasses with E. coli in such a level could be a potential hazard for consumers
RESUMEN
Background: brucellosis is one of the five common bacterial zoonosis caused by a gram negative, non-spore forming, and facultative intracellular bacterial organism belonging to the genus Brucella. Although brucellosis is considered as a health problem for both men and domestic animals in many countries, any licensed human vaccine has not been designed and produced for it yet. To overcome the problem, currently, antigenic determinants of Brucella cell wall e.g. outer membrane proteins [OMPs] and lipopolysaccharide [LPS] are considered as potential candidates to develop subunit vaccines
Materials and Methods: brucella abortus S99 used in the present study is obtained from the standard bacterial collection of Institute Pasteur of Iran. OMPs were extracted by deoxy cholate extraction technique and further purification performed by sequential centrifugation and ultracentrifugation. Protein concentration was determined using the Nano drop ND-10000 spectrophotometer. SDS-polyacrylamide gel electrophoresis [SDS- PAGE] was performed to determine the electrophoretic pattern and the molecular weight of the extracted OMP samples
Results: OMPs concentration of B.abortus S99 has been measured and reported as 6.27 mg/ml. SDS-PAGE analysis indicated one protein band in the range of 36-38 kDa which would be classified as the porins of B.abortus S99
Conclusion: extraction of B.abortus S99 OMPs with the applied method in the present study produced a satisfactory yield of OMPs. These proteins belonging to the second group of OMPs, called porins