RESUMEN
Background: Cyclosporine is an immunosuppressive drug used largely in organ graft medicine with serious effects on liver parenchyma. We experienced combination of cyclosporine and vitamin A for liver damage risk reduction in rats
Materials and Methods: We random allocated 40 rats in 4 study groups and inoculated 1. cyclosporine with olive oil subcutaneously; 2. intramuscular cyclosporine and vitamin A; and 3. subcutaneous absolute olive oil. The 4[th] rat group was not injected and it considered as normal control group. Inoculation was continued for two weeks and rats were kept in standard equal environmental condition. By the end of the injection period they all were dissected and 2 liver tissue samples were studied from each rat using H and E stain and light microscope. Tukey test was used to compare kupfer cells between study groups by considering an alpha level of 0.05 as significant
Results: Hepatocytes disarrangement, congestion and hyperemia of sinusoids, and apoptotic cell enhancement were outcomes of cyclosporine use in rats. There was also blood in kupfer cells and obscure portal space. In comparison with vitamin A addition cyclosporine group showed increasing of kupfer cells [P<0.001]
Conclusion: Adding Vitamin A to cyclosporine regimen can probably results in liver damage risk reduction
RESUMEN
Background: Cyclosporine is an immunosuppressive drug used largely in organ graft medicine with serious effects on liver parenchyma. We experienced combination of cyclosporine and vitamin A for liver damage risk reduction in rats
Materials and Methods: We random allocated 40 rats in 4 study groups and inoculated 1. Cyclosporine with olive oil subcutaneously; 2. intramuscular cyclosporine and vitamin A; and 3. subcutaneous absolute olive oil. The 4[th] rat group was not injected and it considered as normal control group. Inoculation was continued for two weeks and rats were kept in standard equal environmental condition. By the end of the injection period they all were dissected and 2 liver tissue samples were studied from each rat using H and E stain and light microscope. Tukey test was used to compare kupfer cells between study groups by considering an alpha level of 0.05 as significant
Results: Hepatocytes disarrangement, congestion and hyperemia of sinusoids, and apoptotic cell enhancement were outcomes of cyclosporine use in rats. There was also blood in kupfer cells and obscure portal space. In comparison with vitamin A addition cyclosporine group showed increasing of kupfer cells [P<0.001]
Conclusion: Adding Vitamin A to cyclosporine regimen can probably results in liver damage risk reduction