HPLC identification of flavonoid content of plant stains on cotton and nylon fabrics

A simple HPLC method was applied for idenfication of flavonoid aglycones in plant stains. Flavonoids were detected from as low as 1 mg of dried leaves and fresh leaves stains on 2.1 cm[2] of cotton and 3.2 cm[2] nylon fabrics. Plant species were identified by comparing the chromatogram of stain with that of plant extract. This method offers a great help in identifying plant stains in cases of criminal investigation