RESUMEN
Early detection of malignant and dysplastic lesions has been an aim for clinicians. Although leukoplakia is not associated with any special hystopathologic diagnosis, it is considered as a premalignant or precancerous lesion. Human Papilloma virus especially HPV16 and HPV18 has been detected in many oral leukoplakia lesions. The aim of this study is to evaluate the presence of HPV and its genotypes 11, 16, 18, 30s and 50s in common leukoplakia, oral hairy leukoplakia, mild dysplasia and compare it with healthy mucosa. Ten samples of each lesion were prepared and fixed in paraffin blocks. After DNA isolation, PCR was done by chelex-100 method. The results were analyzed by SPSS statistical software, Chi-Square and Fisher's Exact test with P Value of 0.05. Seven out of 10 common leukoplakia and OHL, 1 out of mild dysplasia and healthy mucosa were HPV+. Double comparison by Fisher exact revealed that there was a significant difference in frequency distribution of HPV between OHL and healthy mucosa [p=0./01] and also between OHL and Mild dysplasia[p=0./01].The difference between frequency distribution of HPV and of common leukoplakia and healthy mucosa[p=0./01],also between common leukoplakia and mild dysplasia [p=0./01] was significant, the difference between other groups was not significant. Results of the study suggest that there is no significant correlation between infection of HPV subtypes in the evaluated lesions. In other words, no significant relationship was shown between the infection of special subtype of HPV and common leukoplakia, OHL and mild dysplasia. According to the findings of this study, HPV probably has a role in the pathogenesis of leukoplakia.To investigate the role of various subtypes of this virus in leukoplakia lesions, further studies with more samples are needed
RESUMEN
Dentists encountered to bleeding disorders in dental clinic. So they must be aware of dental management of them. Patients dental and medical evaluation is necessary especially when treatment has potential bleeding risk. To determine of knowledge and behavior of general dental Practitioner about coagulation tests and bleeding disorders in Rasht in year of 2009. In this cross sectional study were evaluated 102 general dentists [66 males and 36 females]. The structured questionnaire was used consisted of 3 sections: first section was about what dental procedure need to more attention? Second section was about laboratory tests and their normal range in dental procedure and third section evaluated behavior of dentists about bleeding disorders. The validity and reliability of questionnaire was confirmed by professors of oral disease department. Data was analyzed by SPSS software and Chi-square Test. Maximum total score was 24 of 25 questions. The mean age of studied group and the duration after graduation time was 38.8 +/- 9.35 and 13.46 +/- 8.1 years respectively.%43.1 of dentists had poor knowledge,%44.1 of them had moderate knowledge and%12.8 of them had good knowledge. The knowledge of dentists was decreased significantly with increasing of age and duration after graduation. The results showed the low knowledge about bleeding disorders between general dentists. So suggested to improve the knowledge of dentists according to training program and congress