Characterization of a reptilian aldolase

The optimum pH of fructose-1, 6-diphosphate aldolase from thigh muscles was found to be 9. The pK values of amino acids present at the active centre were found to be 7 and 9.7 corresponding to Imidazolium group of histidine and E1-ammonium group of lysine, respectively. The Km value and Vmax were found to be 63.49 mM and 10 S-L units, respectively