RESUMEN
Statement of Problem: Due to the clinicopathologic similarity of pyogenic granuloma to some neoplastic or hamartomatouse lesion, vascular density can represent some biological behaviors of this lesion
Purpose: This study aimed to evaluate the density of the vessels and the clinicopathological features of oral pyogenic granuloma [PG] and its histopathologic subtypes
Materials and Methods: Information about age, sex, site, size, duration and the presence of surface ulceration in total and in two histopathologic subtypes [lobular and non-lobular] were collected From 94 PG files in the archives and were compared. Vessel counting was performed on Hand E slides and the density of the vessels was compared in two subtypes, two genders and ulcerated and non-ulcerated groups using Independent Samples T- test
Results: Pyogenic granuloma comprised 4.5 percent of all the lesions. The male to female ratio was 1:1.7. The mean age of the patients was 27.99 years and the most frequent involved site was the gingiva [84%]. The Non-LCH lesions were more than LCH. Post- mandibular gingiva in the LCH group and the anterior portion of the maxillary gingiva were the most locations of involvement. The mean duration of the presence of the lesion in Non-LCH was more than that in LCH. Surface ulceration in Non-LCH [89.4%] was more than LCH [78.6%]. Although the mean density of the vessels was equal in both males and females; there was a significant difference in the lesions with or without surface ulcerations [p =0.01]. However there was no significant difference between the lobular and nonlobular groups
Conclusion: The site of the lesion, duration, and the lower cases with surface ulceration in the LCH form can be in contrast with reactive nature of this subtype, showing similarity to tumoral or developmental lesions. Gender does not influence the density of blood vessels in PG but surface ulceration can influence it
RESUMEN
Hemophilia B is a genetic disorder due to deficiency or complete absence of factor IX coagulation factor. Treatment of choice for these patients is use of factor IX concentrates. Therefore, purification of plasma proteins and separation of factor IX have been major objectives for scientists involved in this field. In this respect, purification procedure using ion exchange chromatography is widely used, but in the past decade affinity chromatography was also introduced. The objective of the present study has been to apply both techniques for the purification of factor IX and compare the quality and yield of the product. For the purification procedure, chromatography columns [XK-16], containing DEAE sepharose and Heparin sepharose were used. Factor IX coagulation activity was measured using a one-stage coagulation assay and factor IX antigen was quantified using ELISA technique. The specific activity and relative increase in purity of factor IX was calculated and it was demonstrated that specific activity improved from 3.1 IU/mg using DEAE ion exchange to 29 IU/mg when affinity chromatography was added and purity was increased from 155 to 1450 respectively. The present study demonstrates that addition of an affinity chromatography step using heparin sepharose is a major improvement in the purification of factor IX, where both specific activity and purity are increased considerably