Assessment of biochemical and antioxidative status in patients suffering from dengue fever / 华中科技大学学报（医学）（英德文版）

A multi-centred study was designed to collect dengue epidemiologic data from government and registered private hospitals/clinics and maintained archive of frozen specimens in bio-bank to be used for future dengue epidemic control program, and assess the epidemiology of dengue fever (DF) by evaluating biochemical and oxidative status of patients. ELISA IgM antibodies test was done to confirm DF. From August 2010 to December 2011, 101 confirmed blood samples of DF patients referred to pathology lab of Jinnah Hospital Lahore were subjected to the epidemiologic assessment by evaluating the biochemical and physiological indices and alterations of circulating antioxidants. Clinical features of DF patients and effect of fever on blood components and serum proteins of liver were recorded. The hospital stay in DF, dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS) showed significant difference. Significant increases in serum alanine amino transferase (ALT) (P=0.000), aspartate amino transferase (AST) (P=0.000), alkaline phosphatase (ALP) (P=0.000), malondialdehyde (MDA) along with significant decreases in total protein (TP) (P=0.000), reduced glutathione (GSH) (P=0.000), superoxide dismutase (SOD), catalase (CAT) (P=0.000), and sialic acid contents (P=0.016) were observed. A positive correlation existed between bound sialic acid levels, liver enzymes and circulating antioxidants (r=0.656, P=0.016). In the present study, alterations of circulating antioxidants in DF suggest that DF might be a metabolic response to an acute, self-limiting tropical viral infection, and a consequence of the viral inflammatory process.

Comparative studies of salivary and blood sialic acid, lipid peroxidation and antioxidative status in oral squamous cell carcinoma [OSCC]

Oral squamous cell carcinoma [OSCC] is considered to be a serious life threatening issue for almost two decades. The objective of this study was to evaluate the over production of lipid peroxidation [LPO] byproducts and disturbances in antioxidant defense system in the pathogenesis of oral cancer. Lipid peroxidation and antioxidant status in OSCC patients were estimated and compared the sensitivity and specificity of circulating biomarkers [MDA, Sialic acid, Catalase, SOD, GSH and Neuraminidase] with B-2 microglobulin [B-2MG] at different thresholds in blood and saliva using receiver operating characteristics [ROC] curve design. Our results showed that the levels of MDA and Sialic acid were significantly increased in plasma of OSCC patients as compared to healthy subjects whereas antioxidant level was significantly decreased. ROC analysis indicated that MDA in saliva is a better diagnostic tool as compared to MDA in blood and B-2MG in blood is better diagnostic marker as compared to B-2MG level in saliva

Polycystic ovarian syndrome; Determination of alterations in glucose level and lipid profile

The objective of the present study was to determine the changes in the glucose level and lipid profile in patients with polycystic ovarian syndrome [PCOS]. Descriptive study. This study was conducted at lnstitute of Molecular Biology and Biotechnology, The University of Lahore from June 2009 to June 2010. Total 50 patients with PCOS were included and 50 age-matched control subjects were also selected for comparison. Their glucose levels and lipid profile were assessed using commercial kits. The data thus obtained was subjected to statistical analysis. Results: Significant differences [P<0.05] in fasting blood glucose level and individual parameters of lipid profile were observed in women with PCOS. A higher prevalence of hypertriglyceridemia, hypercholesterolemia, higher LDL, lower HDL and higher fasting blood glucose levels was explored in PCOS women than controls. Abnormal glucose level and lipid profile in PCOS women showed that these women are at an increased risk of developing diabetes and subsequently cardiovascular diseases.