RESUMEN
Objective: to detect H. Pylori antigen in stool and to determine the diagnostic accuracy of H. pylori stool antigen
Design: comparative analytical study
Setting: this study was carried out at Pakistan Medical Research Council [PMRC], at Jinnah Postgraduate Medical Centre, Karachi from May 2006 to September 2006
Material methods: endoscopic biopsy specimens were collected for rapid urease test and histopathology examination. Stool samples were taken for detection of antigen of Helicobacter pylori. The stool antigen test is a rapid immuno-assay based on "lateral flow chromatography technique" using monoclonal antibodies for qualitative detection of Helicobacter pylori antigen in human stool
Result: fifty males and 50 females were selected for this study by non-probable purposive sampling technique. Their mean age was 39 years ranging from 15 to 55 years? The sensitivity and the specificity of Helicobacter pylori stool antigen test [HPSA] with rapid urease test was found to be 89.1% and 92.6% respectively and the sensitivity and specificity of HPSA test with histopathology was 92.8% and 89.6% respectively
Conclusion: helicobacter pylori stool antigen [HPSA] is an accurate easily carried out in routine laboratory and is non-invasive
RESUMEN
Background/Objectives: An ELISA test based on mycobacterial antigen A60 for the estimation of Anti [A60] IgG in the serum has been used successfully for the rapid diagnosis of tuberculosis in studies done in the European countries, China and India. To demonstrate and evaluate anti-tuberculosis anti-body IgG for rapid diagnosis of both pulmonary and extra pulmonary tuberculosis, this study was carried out in our set up
Methods: ELISA assay based on mycobacterial antigen A60 [Anda Biologicals, France] was used to the sera obtained from 69 cases of tuberculosis and 136 controls in the population of Karachi, Sindh -Pakistan
Results: Of 136 controls, only 17.7% were positive for IgG antibodies and showing 82.3% specificity. A very high sensitivity [88.8%] was seen in sputum positive active pulmonary tuberculosis. Relatively low [75%] sero-positivity was seen in cases of sputum negative active pulmonary tuberculosis compared to those of sputum positive active pulmonary tuberculosis. In cases of extra pulmonary tuberculosis, 47.3% sensitivity was observed. In case of healed tuberculosis quite a large number of cases was found positive for IgG [62.5%]
Conclusion: The estimation value of anti tuberculosis antibody IgG against A60 for the rapid diagnosis is proved from our data. Considering all the cases of active tuberculosis and the controls the global sensitivity of 69.8% and specificity of 82.4% were obtained when IgG antibodies estimation were taken into account