Bioremediation of Zearalenone by using Lactobacillus acidophilus in albino rats bodies [in vivo]

Objective: This study aimed to evaluate the efficiency of Lactobacillus acidophilus to reduce zearalenone toxicity in vital systems of albino white rats

Methods: Fusarium graminearum isolate was tested to produce zearalenone toxin. L. acidophilus was used to reduce the toxin in rats. This ability was studied by many parameters such as biochemical and physiological parameters in addition to histological study

Results: The results showed that L. acidophilus had the ability to reduce zearalenone toxicity. L. acidophilus had the ability to raise Hb 11 g/dl in blood of animals that was treated with zearalenone only to 13.06 g/dl in animal's blood that was treated with zearalenone + L. acidophilus. As well as the number of red blood cells in animals blood treated with these treatment was 6.62 x 10[12] cell/ L. L. acidophilus reduces the toxicity of zearalenone through its ability to reduce the number of platelets to normal levels

Conclusion: L. acidophilus had a role in repeated biochemical parameters to normal levels. Total protein rose to 6.6 g/dl in animal's blood treated with zearalenone + L. acidophilus compared with these levels in blood of control group. Also GPT and sugar levels in animal blood that was treated with zearalenone + L. acidophilus were at normal level. Histological study proved that L. acidophilus had the ability to protect liver, kidney, and uterus from the toxicity of zearalenone

best stain for morphological study of human seminal fluid's smears

There is a high need for proper evaluation of the morphological features of human sperms. The importance of this lies in the field of andrology, male fertility and in vitro fertilization. The wet smears can give rough clue about the shape of the sperms, but it is neither accurate nor reproducible. This study aimed to determine the best stain which can be used for seminal fluid cytology. This study was conducted in Port Sudan, Red Sea State, Sudan in the period from October 2006 to September 2007. The total number of patients was 50. Samples which were collected from normospermic patients [NSP] were prepared by direct smear technique. Samples which were collected from oligospermic patients [OSP] and azoospermic patients [ASP] were prepared by direct smear technique and also by indirect smear techniques [concentration method]. Smear samples were stained by freshly prepared Harris's Haematoxylin, Papanicolaou stain, May-Grunwald Giemsa stains [MGG], supra vital stain, Giemsa stain and leishman's stain. In this study, the best stain was Harris's Haematoxylin [80% excellent for the head of sperm, 70% good for the neck, 59% excellent for the tail, 42% very good for cells in background]. Harris's stain was followed by papanicolaou stain and the third best stain was supra vital stain. MGG was better than Giemsa in staining of semen smears [75% good versus 25% good] in overall performance. The worst stain was Leishman's stain. Stained smears must be used for the morphological study of semen samples. Harris's Haematoxylin is the best stain for semen cytological features. Stains which used for the semen samples should be freshly prepared