prognostic significance of proliferative factors, angiogenesis and matrix metalloproteinase in non small cell lung carcinoma

The outcome of surgical treatment of non small cell lung carcinoma [NSCLC] remains poor. In many patients the biological behavior of NSCLC does not follow a definite pattern, and can not be accurately predicted before treatment. Thus, the proliferative potential of tumor cells, angiogenesis and matrix metalloproteinase [MMP] are an important prognostic factors. In the present study, expression of cyclin D1, CD34, MMP-2 and AgNORs count was estimated in a group of 80 surgically resected NSCLC using immunohistochemistry. The results were compared with clinicopathological parameters including patients' survival 41 cases [51.3%] revealed positive immunoreactivity for cyclin D1. Cyclin D1 overexpression is significantly higher [<0.05] in patients with lymph node metastasis [63.3% versus 15%], and with advanced pathological stages [stage I, 12.5%, II 37.5%, III 60% and IV 86.7%]. Patients with cyclin D1 positive immanoreactivity revealed a significantly shorter overall survival than patients with negativity. There is no significant correlation [p > 0.05] between CD34 score and histological type and grade, while there is significant positive correlation [p < 0.05] between high CD34 score and lymph node metastasis, distant metastasis, tumor stage and shorter over survival. No significant correlations [p > 0.05] were found between MMP-2 expression and histological type, grade and lymph node metastasis. In contrast the intensity of MMP-2 staining in tumor cells correlated significantly [p < 0.05] with tumor stage, and distant metastasis. Overall survival was shorter in patient with MMP-2 expression, although the difference does not reach statistical significant AgNORs count was found to correlate significantly [p < 0.05] with tumor grade, and the size, shape and distribution pattern was found to show a characteristic difference between non-neoplastic and neoplastic lesions. In conclusion, an overexpression of cyclin D1, angiogenesis, expression of MMP-2 and high AgNORs count are a poor prognostic factors for NSCLC, also MMP-2 and AgNORs can be used to differentiate between non-neoplastic and neoplastic lung lesions

Expression of cell regulatory proteins C-erb B-2 and Bcl-2 in epithelial ovarian tumors

Ovarian cancer remains the leading cause of death from gynecologic malignancy in many countries. Lack of symptoms in the early stages of disease with consequent delay in diagnosis continues to result in poor overall survival. The expression of cell regulatory proteins was studied in an attempt to find the role of these proteins in differential diagnosis and progression of ovarian tumors. Sixty five cases with epithelial ovarian tumors [35 serous, 24 mucinous, 4 cases endometroid carcinomas and 2 cases were undifferentiated tumors were evaluated for expression of c-erbB-2 and bcl-2 by immunohistochemical technique and correlated with clinical parameters such as type, grade, and stage-Expression of c-erbB2 was significantly differed among the various types of ovarian tumors [p=0.01], highly expressed in undifferentiated, high grade, and advanced stage tumors in contrast to its weak expression or loss in benign, variety. While the bcl-2 was significantly lost in more aggressive tumors but strong expression was observed in benign lesions .p=0.01. In ovarian tumors, proto-oncogene c-erbB-2 and antiapoptotic gene bcl-2 had a role in behavior and differentiation of these tumors