RESUMEN
Objective: To use one of such mechanism like bacteriocins, produced by Lactobacilli activities against pathogens
Study Design: Analytical / observational study
Place and Duration of Study: This study was carried out at Gulab Devi Chest Hospital, Lahore from November 2012 to January 2013
Materials and Methods: This study included 203 clinical samples. Multidrug resistant clinical isolates were selected on the basis of their MAR [Multiple antibiotic resistances] index, antibiotic susceptibility testing, methicillin resistant Staphylococcus aureus [MRSA] with oxacillin disc, double disc synergism and combination disc test. Plasmid isolation, conjugation was performed. Well-Diffusion assay was used for screening of putative bacteriocins produced by Lactobacillus strains against MDRs. Physiological characterization of antimicrobial compounds and protein estimation was analyzed
Results: Twenty five strains were selected based on MAR index [>0.2]. In which 6 MRSA and 19 extended spectrum beta-lacatmases [ESBL] producers were further proceeded for antimicrobial activity with putative bactericins. Plasmid was easily transferred their resistance by the process of conjugation. Five bacteriocins were obtained from Lactobacillus strains isolated from commercial products. These bacteriocins showed a strong antibacterial activity against selected MDRs. Decrease in zone sizes was observed when putative bacteriocins were treated with heat, SDS [Sodium dodecyl sulfate] and Protinase k. Putative bacteriocins produced by Lactobacilli exhibit significant antibacterial activity against MDRs. SA1 has high antibacterial activity with high protein content of 13mg/ml
Conclusion: Putative bacteriocins produced by Lactobacilli exhibit significant antibacterial activity against selected MDRs
MDRs have ability to transfer their resistance to other bacteria. The peptidal component of these bacteriocins can be used as an alternative therapy. Proper hospital policies require minimizing the horizontal spread of MDRs. Hence, it is necessary to purify the antibacterial molecule out of putative bacteriocin for further analysis