RESUMEN
This study was carried out to investigate fifteen cases of acute lethal infection of calves (< or = 4 months of age) by the protozoan parasite Theileria (T.) annulata in the south of Portugal. Calves developed multifocal to coalescent nodular skin lesions, similar to multicentric malignant lymphoma. Infestation with ticks (genus Hyalomma) was intense. Theileria was seen in blood and lymph node smears, and T. annulata infection was confirmed by isolation of schizont-transformed cells and sequencing of hypervariable region 4 of the 18S rRNA gene. At necropsy, hemorrhagic nodules or nodules with a hemorrhagic halo were seen, particularly in the skin, subcutaneous tissue, skeletal and cardiac muscles, pharynx, trachea and intestinal serosa. Histologically, nodules were formed by large, round, lymphoblastoid neoplastic-like cells. Immunohistochemistry (IHC) identified these cells as mostly CD3 positive T lymphocytes and MAC387 positive macrophages. A marker for B lymphocytes (CD79alphacy) labeled very few cells. T. annulata infected cells in these nodules were also identified by IHC through the use of two monoclonal antibodies (1C7 and 1C12) which are diagnostic for the parasite. It was concluded that the pathological changes observed in the different organs and tissues were caused by proliferation of schizont-infected macrophages, which subsequently stimulate a severe uncontrolled proliferation of uninfected T lymphocytes.
Asunto(s)
Animales , Bovinos , Femenino , Masculino , Secuencia de Bases , Enfermedades de los Bovinos/epidemiología , Procesos de Crecimiento Celular/fisiología , ADN Protozoario/química , Brotes de Enfermedades/veterinaria , Inmunohistoquímica/veterinaria , Linfocitos/parasitología , Datos de Secuencia Molecular , Reacción en Cadena de la Polimerasa/veterinaria , Portugal/epidemiología , ARN Ribosómico 18S/química , Análisis de Secuencia de ADN , Enfermedades de la Piel/epidemiología , Theileria annulata/aislamiento & purificación , Theileriosis/epidemiologíaRESUMEN
Desenvolve algumas reflexoes de carater mais conceitual a respeito do ecletismo - "estilo" ao qual esta associado o conjunto arquitetonico de Manguinhos - bem como uma analise historica dos processos de producao dos bens arquitetonicos e do espaco urbano a epoca em que se edificou o Instituto Oswaldo Cruz. Trata, ainda, mais especificamente, do Instituto e de seu arquiteto, Luiz de Morais Junior. Aborda tambem a regiao onde esta localizado o Instituto, indicando em linhas gerais como evoluiu a ocupacao da area.