The Effect of 3-Dimensional Microcarrier Culture on Chondrocyte Proliferation and Matrix Production / 대한정형외과연구학회지

PURPOSE: Injury to articular cartilage is likely progressive to develop arthritis and dysfunction of joint because its regeneration is limited. Transplantation of cultured autologous chondrocytes was proved to be a satisfactory treatment method and could prevent a dysfunction of joint. However chondrocytes propagated in monolayer culture lose their original characteristics by assuming a morphologic shift to fibroblast like cell and biosynthetic shift to production of collagen type I from type II and to decrease in synthesis of DNA and proteoglycans. A three dimensional culture of articular chondrocytes using microcarrier was performed to overcome disadvantages of phenotypic changes in monolayer culture. MATERIALS & METHODS: Cultured articular chondrocytes, 6 times passed in 13 weeks, were innoculated and propagated in flasks for monolayer culture and spinner flasks for three dimensional culture respectively for 2 weeks. RESULTS: Cells in three dimensional culture proliferated faster and showed more markedly increase in cell number than those in monolayer culture. Expression of type II collagen was observed in three dimensional culture, but was hardly observed in monolayer culture. CONCLUSION: Degree of synthesis of DNA and proteoglycans also more markedly increase in three dimensional culture than in monolayer culture. Our experiments demonstrate that three dimensional culture of articular chondrocyte using microcarrier provides increase in growth and reexpression of phenotype of chondrocytes.

The Effect of 3-Dimensional Microcarrier Culture on Chondrocyte Proliferation and Matrix Production / 대한정형외과연구학회지

PURPOSE: Injury to articular cartilage is likely progressive to develop arthritis and dysfunction of joint because its regeneration is limited. Transplantation of cultured autologous chondrocytes was proved to be a satisfactory treatment method and could prevent a dysfunction of joint. However chondrocytes propagated in monolayer culture lose their original characteristics by assuming a morphologic shift to fibroblast like cell and biosynthetic shift to production of collagen type I from type II and to decrease in synthesis of DNA and proteoglycans. A three dimensional culture of articular chondrocytes using microcarrier was performed to overcome disadvantages of phenotypic changes in monolayer culture. MATERIALS & METHODS: Cultured articular chondrocytes, 6 times passed in 13 weeks, were innoculated and propagated in flasks for monolayer culture and spinner flasks for three dimensional culture respectively for 2 weeks. RESULTS: Cells in three dimensional culture proliferated faster and showed more markedly increase in cell number than those in monolayer culture. Expression of type II collagen was observed in three dimensional culture, but was hardly observed in monolayer culture. CONCLUSION: Degree of synthesis of DNA and proteoglycans also more markedly increase in three dimensional culture than in monolayer culture. Our experiments demonstrate that three dimensional culture of articular chondrocyte using microcarrier provides increase in growth and reexpression of phenotype of chondrocytes.

The Results of Five Years Follow-Up of Cemented Total Knee Arthroplasty

PUEPOSE: To analysis the clinical and radiographic results of patients who could be followed more than five years in the cemented total knee arthroplasty. MATERIALS AND METHODS: Twenty-two cases(30 patients) were analysed clinically and radiographically, which underwent cemented total knee arthroplasties between March 1991 and December 1994, and were followed up at least more than 5 years. Several factors such as pain at walking, range of motion(ROM), knee rating score of the Hospital for Special Surgery(HSS), flexion contracture for clinical evaluation were evaluated and femorotibial angle, radiologic component position, radiolucent zone according to the roentgenographic evaluation of american knee society for the radiologic evaluation were analysed. RESULTS: Twenty-nine cases(90.7%) were rated as good or excellent, HSS scores were improved from preoperatively 51.5 points to postoperatively 85.5 points, Flexion contracture was relieved from 11degrees pre-operatively to 1degree postoperatively and ROM of knee joint were increased from 118degrees to 120.2 degrees . There were 4 complications; patellar subluxation in three cases, aseptic loosening in one case. The survival rate was 96.9% at six years and seven months. CONCLUSION: According to the clinical and radiologic assessments, cemented total knee arthraplasty seemed to be one of the best methods to fix the prostheses of total knee arthroplasty, which gave good or excellent clinical results at more than five years follow up.