1.
RBCF, Rev. bras. ciênc. farm. (Impr.)
;
40(3): 301-308, jul.-set. 2004. ilus, tab, graf
Artículo
en Inglés
| LILACS
| ID: lil-420677
RESUMEN
This paper describes validated direct liquid chromatographic chiral methods for enantiomeric separation and quantitative determination of clinically significant ß-blocking agent, propranolol. A liquid chromatographic method was validated and applied for enantiomeric determination of propranolol enantiomers in pharmaceutical formulations. Separation were obtained in polar organic mode on a `alpha'-Burke 2® chiral stationary phase (250 x 4.6 mm, 5 µm) with mobile phase composed of dichloromethane:methanol (90:10 v/v), along with 12 mM of ammonium acetate, at a flow rate of 0.9 mL/min. Detection was made by ultraviolet absorption at 280nm. In all cases the run time was less than 10 min...