RESUMEN
A pilot study was conducted to differentiate various brands of alcoholic beverages commonly sold in Hyderabad city (Andhra Pradesh, India). Various brands of liquor were scanned up to parts per billion (PPB) level for more than 72 elements using the following method – Inductively Coupled Plasma Atomic Emission Spectroscopy (ICP-AES). The results obtained showed significant difference in the elemental profiles of various brands of liquor. Absence of some elements such as copper showed marker parameter for the liquor brands. This survey shows that trace elemental profiling could be a reliable method for differentiating various brands of liquor.
RESUMEN
A new gas chromatography-mass spectroscopy (GC-MS) method is being presented for the separation and detection of strychnine and brucine, alkaloids of Strychnos nux vomica in a single run. The analysis was carried out using 5% phenyl methyl silicone capillary column, electron impact ionization mode and quadrupole mass analyzer. The extracts of the exhibits were analyzed using the new method. The peaks of the two alkaloids were found to be well resolved, and there was clear separation between the two. The retention time and mass fragmentation pattern, base peaks, molecular peaks of strychnine and brucine standard/NIST library and crime case exhibits matched, establishing the presence of the two active principles of Strychnos nux vomica. The new method has the advantage of better separation of the two alkaloid peaks over the conventional GC-MS methods, and is useful for the identification and confirmation of Strychnos nux vomica constituents in biological matrices of poisoning cases that have ended in death.
RESUMEN
There have been a variety of approaches developed since the 1970s to detect pyrethroid pesticides based on gas chromatography (GC), thin layer chromatography (TLC), and high performance liquid chromatography (HPLC) with ultraviolet detection. A new thin layer chromatographic method was developed for the detection of common pyrethroids in forensic toxicology case exhibits. Various solvent systems were tried as mobile phase with different spray reagents; picric acid was used as specific spray reagent for deltamethrin, fenvalerate, and cypermethrin. The method developed is rapid and sensitive and can be used for routine case analysis with better separation. A new modified spray reagent was developed for the detection of synthetic pyrethroids containing a hydrolysable nitrile group such as deltamethrin, fenvalerate and cypermethrin using picric acid, that produces reddish-orange spots on a yellow background.
RESUMEN
A 13 year-old child suffered an anaphylactic reaction to a drug combination and died in the clinic. The Investigating Officer collected an assortment of drugs and injection vials from the clinic and forwarded them along with the injection site of the victim's skin and some viscera to the laboratory for examination. At the laboratory, the samples were subjected to chemical and chromatographic analysis. After extracting the drug from the biological samples, TLC was done using a new solvent system, which improved the separation of drug from the matrix. A gas chromatographic-mass spectrometric (GC-MS) method devised for the determination of paracetamol residue in skin tissues was done. This method allows for detection of residual drug in biological tissues by using single-ion monitoring, and confirmation by a full scan electron impact (El) mass spectrum. Paracetamol was extracted with ether/chloroform from the samples after acidic and basic extraction procedures, cleaned up and washed, followed by partition between chloroform. The cleaned up extract was injected into the GC-MS, and detection was done using single ion monitoring at m/z 109.