RESUMEN
To investigate the ameliorating role of pomegranate on minocycline induced pigmentation in the epidermis of guinea pig. An experimental observational study. This study was conducted at the Anatomy Department, BMSI, J PMC, Karachi. 30 adult guinea pigs were divided into 3 groups A B and C. In this study, A served as control, B was given Minocycline, while C was given Minocycline with Pomegranate for 8 weeks, after which their skin was processed for histological examination of morphology of melanocytes and pigmentation in Dopa Oxidase stained sections under light microscope. The melanin pigmentation deposition in Minocycline treated group B was distributed densely and extended till stratum corneum as compared to the control group A, while in the Pomegranate treated group C along with Minocycline, the melanin pigmentation was considerably reduced and was observed to be distributed sparsely extended till stratum basale. The morphology and number of melanocytes in both treated groups remain same as compared to control group A. Based on the present study it is concluded that pigmentation induced by Minocycline can be reduced and may be prevented by taking pomegranate simultaneously
RESUMEN
To investigate the protective effects of Pomegranate on Minocycline induced epidermal pigmentation on the extremities of guinea pigs. An experimental observational study. This study was conducted at the Anatomy Department, BMSI, J PMC, Karachi. 60 adult guinea pigs were divided into 3 groups A B and C, A served as control, B given Minocycline, while C given Minocycline with pomegranate for 8 weeks, after which their skin was processed for histological examination and pigmentation was observed in Masson Fontana stained sections under light microscope. The melanin pigmentation deposition observed in Minocycline treated group B, distributed densely and extended till stratum corneum as compared to the control group A, while in the Pomegranate treated group c along with Minocycline, the melanin pigmentation was considerably reduced and was observed to be distributed sparsely extended till stratum spinosum. Based on the present study it is conducted that pigmentary changes induced by Minocycline can be protected by taking pomegranate