Susceptibility of Anopheles anthropophagus from different areas of China to Plasmodium vivax / 中国血吸虫病防治杂志

Objective To compare the susceptibility of Anopheles anthropophagus from Jiangsu, Guangdong and Liaoning provinces in China to Plasmodium vivax.Methods The blood samples of patients with P. vivax in endemic areas of China were collected to feed the mosquitoes of An. anthropophagus from different areas by using the artificial in vitro membrane feeding system in the lab, and then the mosquitoes were dissected during the 7-9th day and on the 14th day after the feeding and the oocysts and sporozoites in the stomach and salivy gland of mosquitoes were counted. Results The mosquitoes from Jiangsu, Guangdong and Liaoning were simultaneously fed with the blood of 35 cases of P. vivax. The oocyst positive rates of An. anthropophagus from Jiangsu, Guangdong and Liaoning during the 7-9th day after the feeding were 68.57%, 60.00% and 68.57%, as well as the sporozoite positive rates of them on the 14th day after the feeding were 22.86%, 14.29% and 22.86%, respectively. On the 7th day after the feeding, 228, 235, 228 mosquitoes of An. anthropophagus from Jiangsu, Guangdong and Liaoning were dissected, and the positive mosquito rates with oocyst infection were 28.07%, 25.11% and 26.75%, respectively. On the 14th day after the feeding, 150, 142, 135 mosquitoes of An. anthropophagus from the three areas were dissected, the positive rates with sporozoite infection were 10.67%, 8.45% and 11.85%, respectively. The num-bers of mosquitoes dissected with infective grade("+","++","+++","++++") of sporozoites of An. anthropophagus from Jiangsu, Guangdong and Liaoning were 4, 3, 2, 7; 2, 2, 3, 7 and 1, 6, 3, 8, respectively. Conclusions An. anthropophagus from Jiangsu, Guangdong and Liaoning is susceptible to the parasites of Plasmodium vivax and there is no significant difference among the susceptibilities of An. anthropophagus from the three areas to Plasmodium vivax.

Growth difference of Plasmodium vivax in Anopheles sinensis and An.anthropophagus during the periods of clinic attack and diapause of vivax malaria patients / 中国血吸虫病防治杂志

Objective To compare the growth of Plasmodium vivax in Anopheles sinensis(An.s) and Anopheles anthropophagus(An.a) during the periods of malaria clinic attack and diapause. Methods The blood samples of patients during the clinic attack and diapause of vivax malaria patients in the vivax epidemic area in China were collected, feeding the mosquitoes of An.s and An.a by using the artificial membrane feeding system in vivo in the lab, and the mosquitoes were dissected during the day 7-9th and 14th after the infection and the oocysts and sporozoites in the stomach and gland of the mosquitoes were counted, respectively. Results The oocyst positive rate in An.s fed by Plasmodium vivax during the fever stage was lower than that in non-fever stage, the sporozoite positive rate in An.a fed by Plasmodium vivax during the fever stage was lower than that in non-fever stage. The positive mosquito rate with oocyst and sporozoite infected by Plasmodium vivax in the fever stage to An.s and An.a were lower than those in non-fever stage. The infective sporozoite intensity of An.s fed by Plasmodium vivax in the fever stage was lower than that in non-fever stage, but the reverse result was found to An.a. Conclusion There is a significant difference between the periods of malaria clinic attack and diapause of the oocyst and sporozoite infection to An.s and An.a.