RESUMEN
The objective of the present study was to evaluate the specificity of the Montenegro skin test (MST) in an area in Brazil, state of Grande do Sul State (RS), which was considered to be non-endemic for leishmaniasis. Sixty subjects presented a positive MST and were reevaluated by clinical examination, serology and polymerase chain reaction (PCR) of peripheral blood for the detection of subclinical Leishmania infection. None of the subjects presented clinical signs or symptoms of current leishmaniasis or a history of the disease.Leishmania (Viannia) DNA was detected in blood by PCR and hybridization in one subject. The PCR skin test-positive individual remained asymptomatic throughout the study. Clinical examination showed no scars suggestive of past cutaneous leishmaniasis. Human subclinical infection with Leishmania (Viannia) in RS was confirmed by PCR. This is the first report of subclinical infection with this parasite in the human population of this area.
Asunto(s)
Animales , Humanos , Leishmania braziliensis , Leishmaniasis Cutánea/diagnóstico , Estudios de Casos y Controles , ADN Protozoario/sangre , Leishmania braziliensis/genética , Leishmania braziliensis/inmunología , Leishmaniasis Cutánea/parasitología , Reacción en Cadena de la Polimerasa , Sensibilidad y Especificidad , Pruebas Cutáneas/métodosRESUMEN
Descreve-se a ocorrência da leishmaniose tegumentar em cães da localidade de Morada das Aguias (Serra da Tiririca), Maricá, Estado do Rio de Janeiro, Brasil. Foram avaliados 83 cães por meio de exames clínico, sorológico e parasitológico. Os soros de 11 (13,2 por cento) animais foram reagentes à imunofluorescência indireta (IFI) e de 30 (36,1 por cento) ao ensaio imunoenzimático (ELISA). Ulceras cutâneas e ou mucosas foram observadas em 18 (n = 83; 21,7 por cento) dos animais. Leishmania foi isolada de 11 cães. Discute-se a ocorrência da doença e a ocupação da localidade.