RESUMEN
Characterizations of the VP4 (P type) and VP7 (G type) genes of Korean isolates of bovine rotavirus were performed using RT-PCR/RFLP and nucleotide sequencing analysis. After RT-PCR amplification of partial length (1094bp) of the VP4 and full length (1062bp) of the VP7 genes, amplified PCR products were digested with restriction endonucleases and digestion patterns were compared with those of reference rotaviruses. With the VP4 genes, four RFLP (AD) profiles were observed; three (A, B and C) were the same as those of bovine rotavirus NCDV (P[1]), IND (P[5]) and B223 (P[11]), respectively, Profile D was the same as that of porcine rotavirus OSU (p[7]). With the VP7 genes, five RFLP profiles (I-V) were observed; three of them (1, II and III) were the same as those of bovine rotavirus NCDV (G6), Cody I-801 (G8), and B223 (G10), respectively, Profile IV and V were atypical to those of reference bovine rotaviruses used in this study. These two profiles were identified as G6 and G5, respectively, after analyzing and comparing the nucleotide sequences. The G typing analysis revealed that 61.9% (26/42) were G6, which included G6 subtype; 28.6% (12/42) were G5; 7.1% (3/42) were G10; 2.4% (1/42) were G8. The P typing analysis revealed that 54.8% (23/42) were P(5); 28.6% (12/42) were P(7); 11.8% (5/42) were P(11); 4.8% (2/42) were P(1). Our results showed that G6/P(5) were the most prevalent rotaviruses in diarrheic calves in Korea. Also, this is the first report that G5P(7) rotaviruses were identified from cattle with diarrhea.
Asunto(s)
Animales , Bovinos , Secuencia de Bases , Diarrea , Digestión , Enzimas de Restricción del ADN , Corea (Geográfico) , Reacción en Cadena de la Polimerasa , Polimorfismo de Longitud del Fragmento de Restricción , RotavirusRESUMEN
Idiopathic gingival fibromatosis is associated with other abnorma ities of which the most striking is hypertrichosis of variable degree. Gorlin and Pindborg described this condition as a distinct syndrome termed idiopathic gingival fibromatosis and hypertrichosis. We report a case of idiopathic gingival fibromatosis and hypertrichosis occuring in 34-year-old woman with enlarged gingival tissue, hypertrichosis, and enlargenment of the breasts, ears and nose. Laboratory tests including hormonal and chromosomal studies were all within normal limits. Histologically, the gingival hyperplastic tissue showed thick bundle of collagen fibers with few fibroblasts or capillaries and little or no inflammatory infiltrate.
Asunto(s)
Adulto , Femenino , Humanos , Mama , Capilares , Colágeno , Oído , Fibroblastos , Fibromatosis Gingival , Hipertricosis , Nariz , Huelga de EmpleadosRESUMEN
Idiopathic gingival fibromatosis is associated with other abnorma ities of which the most striking is hypertrichosis of variable degree. Gorlin and Pindborg described this condition as a distinct syndrome termed idiopathic gingival fibromatosis and hypertrichosis. We report a case of idiopathic gingival fibromatosis and hypertrichosis occuring in 34-year-old woman with enlarged gingival tissue, hypertrichosis, and enlargenment of the breasts, ears and nose. Laboratory tests including hormonal and chromosomal studies were all within normal limits. Histologically, the gingival hyperplastic tissue showed thick bundle of collagen fibers with few fibroblasts or capillaries and little or no inflammatory infiltrate.
Asunto(s)
Adulto , Femenino , Humanos , Mama , Capilares , Colágeno , Oído , Fibroblastos , Fibromatosis Gingival , Hipertricosis , Nariz , Huelga de EmpleadosRESUMEN
Annular elastolytic giant cell granuloma(AEGC(3) has been called as atypical necrcibiosis lipoidica, Mieschers granuloma of the face and scalp, and actinic granu- Joma and needs differen(iation from granuloma annulare, elastosis perforans serpiginosa and nerrobiosis lipoidica. We report a case of AECCG occurring in a 53-year-old male. The patient. had an annular erythematous plaque measuring 4 cm in diameter with serpiginous raised edges and slightly atrophic centers on the right forearm for 5 years. The histopathologic finding showed patchy dermal infiltration of lymphocytes, histiocytes, and multinucleated giant cells engulfing elastic fibers. The. skin lesion cleared after intrader mal injection cf cortieosteroids.
Asunto(s)
Humanos , Masculino , Persona de Mediana Edad , Actinas , Tejido Elástico , Antebrazo , Células Gigantes , Granuloma , Granuloma Anular , Granuloma de Células Gigantes , Histiocitos , Linfocitos , Cuero Cabelludo , PielRESUMEN
No abstract available.