Expression of c-jun by X-ray According to Cell Growth State in CaSki Cell Line / 대한방사선종양학회지

PURPOSE: The expression pattern of c-jun by ionizing radiation according to cell growth state (exponential growth phase vs. stationary phase) and its relationship with cell cycle redistribution were investigated. MATERIALS AND METHODS: The exponential growth phase (day 4) and stationary phase (day 9) cells were determined from cell growth curve according to the elapse of days in CaSki. The cells were irradiated using 6 MV X-ray with a dose of 2 Gy at a fixed dose rate of 3 Gy/min. Northern blot analysis was performed with total cellular RNA and cell cycle distribution was analyzed using flow cytometry according to time-course after irradiation. RESULTS: The maximum expression of c-jun occurred 1 hour after irradiation in both exponential growth and stationary phase cells. After then c-jun expression was elevated upto 6 hours in exponential growth phase cells, but the level decreased in stationary phase cells. Movements of cells from G0-G1 to S, G2-M phase after irradiation were higher in exponential growth phase than stationary phase. CONCLUSION: c-jun may be involved in the regulation of cellular proliferation according to the growth states after irradiation.

Palliative Radiotherapy for Brain Metastases / 대한방사선종양학회지

PURPOSE: Radiotherapy has been the mainstay of the treatment of brain metastases. We evaluated the response rate, survival and prognostic factors of patients with brain metastases treated with radiotherapy for palliative purpose. MATERIALS AND METHODS: From January 1994 through April 1997, in all 42 patients, a retrospective analysis was undertaken. Of these, 33 patients received whole brain irradiation with 30Gy in 10 daily fractions with or without a boost of 10Gy in 5 daily fractions to the site of solitary lesion. Nine patients failed to complete the planned treatment. RESULTS: Of 33 patients who finished radiotherapy, complete and partial response were observed in 4 (12%) patients and 22 (67%) ones, respectively. Overall response rate was 79% and median survival was 4 months. In univariate analysis, prognostic factors affecting survival were initial neurologic function class (p=0.0136), extracranial tumor activity (p=0.042), and response after radiotherapy (p=0.001). CONCLUSION: We confirmed that whole brain irradiation is the effective means for treating the patient with brain metastases. Initial neurologic function class, extracranial tumor activity, and response after radiotherapy were identified as prognostic factors affecting survival.