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1.
Artículo en Inglés | IMSEAR | ID: sea-110493

RESUMEN

BACKGROUND: There is high prevalence of tuberculosis in patients with HIV infection; hence the role of non-tuberculous mycobacteria (NTM) in HIV patients has always been undermined. NTM may be responsible for clinical disease in a substantial number of immuno-compromised HIV sero-positive individuals even in a country endemic for Mycobacterium tuberculosis (M. tuberculosis). The study was designed to look for the contribution of NTM to morbidity in HIV seropositive patients. MATERIAL AND METHODS: In a prospective study of ninety-four HIV seropositive individuals presenting with pulmonary or extra-pulmonary symptoms suggestive of mycobacterial infection, appropriate samples were collected and processed. Detailed clinical history was utilized to differentiate colonization or contamination by NTM from true lung disease. RESULTS: Fourteen samples grew mycobacterial species, 8(57.2%) being NTM. The distribution of NTM was--3 M. avium complex, 2 M. fortuitum, 2 M. vaccae, 1 M. phlei. 6 isolates were M. tuberculosis. CONCLUSION: NTM may be responsible for a significant proportion of mycobacterial infections in HIV seropositive individuals. Despite the high endemicity of tuberculosis in developing countries like India, the presence of NTM should be ruled out; especially in immuno-compromised HIV seropositive individuals before instituting anti-tubercular therapy empirically. In addition, non-response of NTM to ATT may be wrongly attributed to multi-drug resistant tuberculosis.


Asunto(s)
Adolescente , Adulto , Femenino , Seropositividad para VIH/complicaciones , Humanos , Masculino , Pruebas de Sensibilidad Microbiana , Persona de Mediana Edad , Infecciones por Mycobacterium/complicaciones , Complejo Mycobacterium avium/aislamiento & purificación , Mycobacterium fortuitum/aislamiento & purificación , Mycobacterium phlei/aislamiento & purificación , Estudios Prospectivos
2.
Southeast Asian J Trop Med Public Health ; 2004 Mar; 35(1): 144-6
Artículo en Inglés | IMSEAR | ID: sea-30702

RESUMEN

This study was designed to evaluate the absolute CD4+ counts and percentages in HIV subtype C infected patients at a tertiary care hospital in northern India. The CD4+ counts of 377 HIV seropositive subjects were estimated by a FACS Calibur (BD) flow cytometer. Dual color immunophenotyping was performed on each sample, which was acquired and analysed using CellQUEST software. Discordance between CD4+ counts and percentages were found more in the early stage ie Group A (37.2%) when compared with Group B (31.6%) and Group C (28.8%), with the counts remaining in the normal range but percentages being severely depressed.


Asunto(s)
Adolescente , Adulto , Anciano , Fármacos Anti-VIH/uso terapéutico , Biomarcadores/análisis , Recuento de Linfocito CD4 , Relación CD4-CD8 , Linfocitos T CD4-Positivos/inmunología , Estudios de Cohortes , Femenino , Citometría de Flujo , Infecciones por VIH/diagnóstico , Seropositividad para VIH/diagnóstico , Humanos , India/epidemiología , Masculino , Persona de Mediana Edad , Monitoreo Fisiológico , Pronóstico , Sensibilidad y Especificidad , Índice de Severidad de la Enfermedad , Distribución por Sexo , Resultado del Tratamiento , Carga Viral
3.
Artículo en Inglés | IMSEAR | ID: sea-23024

RESUMEN

We report the HIV seropositivity among adult TB patients from our hospital, a tertiary care hospital in north India between 2000-2002. Of the 555 patients with various forms of tuberculosis, 52 were found to be seropositive (9.4%). In 1994-1999, the HIV seropositivity in this hospital was only 0.4 per cent (2 of 500 patients). This communication describes a dramatic increase in seropositivity and highlights the importance of continued HIV serosurveillance in patients with TB.


Asunto(s)
Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Niño , Femenino , Seropositividad para VIH/complicaciones , Seroprevalencia de VIH/tendencias , Humanos , India/epidemiología , Masculino , Persona de Mediana Edad , Tuberculosis Pulmonar/complicaciones
4.
Indian J Med Microbiol ; 2003 Apr-Jun; 21(2): 93-7
Artículo en Inglés | IMSEAR | ID: sea-54071

RESUMEN

PURPOSE: The objective of this study was to clone a c-DNA fragment of hepatitis C virus in a eukaryotic expression vector and to measure the efficacy of humoral immune responses in mice inoculated with this recombinant plasmid. This study was an attempt to lay a foundation for HCV nucleic acid vaccine development in the future. METHODS: A c-DNA fragment of BK146, a clone of HCV type 1b, was sub-cloned into an eukaryotic expression vector pMT3. HepG2 and COS cells were transfected with this construct, named pMT3-BK146. The expression of HCV mRNA and proteins was studied by reverse transcribed polymerase chain reaction, radio Immunoprecipitation (RIPA) and immunofluorescence (IFA). The DNA of this construct was injected into the footpad of BALB/c mice and antibody response was tested by enzyme immunoassay and indirect immunofluorescence. RESULTS: COS and HepG2 cells transiently transfected with the recombinant plasmid pMT3-BK146 showed the expression of HCV proteins by RT-PCR, RIPA and immunofluorescence. This DNA clone when injected into Balb/c mice was able to generate specific antibody response to hepatitis C virus by ELISA and IFA. CONCLUSIONS: A c-DNA fragment of HCV cloned in an eukaryotic expression vector was able to express core protein. This DNA clone was also able to elicit antibody response in mice. This can be an initial step towards the development of a potential DNA vaccine for hepatitis C virus infection.

5.
J Postgrad Med ; 2002 Jan-Mar; 48(1): 27-8
Artículo en Inglés | IMSEAR | ID: sea-116058

RESUMEN

BACKGROUND: Difference of opinion about the prevalence of H. pylori association with gastric cancer exists in the literature. AIMS: To study the correlation of Helicobacter pylori (H. pylori) to gastric carcinoma. METHODS: 50 proved cases of gastric cancer were studied by rapid urease test, culture, histopathology and ELISA test for H. pylori IgG. RESULTS: 68% of cases of gastric cancer were found to be positive for H. pylori infection as compared to 74% of healthy controls. CONCLUSIONS: The prevalence rate of H. pylori infection in our patients of gastric cancer was lower than in the control population though statistically not significant, suggesting that H. pylori may not be responsible for gastric carcinogenesis in this population.


Asunto(s)
Adulto , Estudios de Casos y Controles , Ensayo de Inmunoadsorción Enzimática , Femenino , Infecciones por Helicobacter/complicaciones , Helicobacter pylori/aislamiento & purificación , Humanos , Masculino , Persona de Mediana Edad , Prevalencia , Neoplasias Gástricas/microbiología
6.
Southeast Asian J Trop Med Public Health ; 2001 Sep; 32(3): 472-5
Artículo en Inglés | IMSEAR | ID: sea-35878

RESUMEN

Dengue fever (DF) is endemic in India and dengue hemorrhagic fever (DHF) has been reported with increasing frequency in the last decade. We evaluated three commercial assays for detection of antibodies to dengue virus, to assess their performance in a diagnostic laboratory. Sera from 58 patients collected during a febrile outbreak in New Delhi in 1997 were studied. The methods evaluated were MRL Diagnostic Dengue Fever Virus IgM Capture ELISA, Pan Bio Dengue Duo IgM and IgG Capture ELISA and Pan Bio Rapid Immunochromatographic test. The MRL ELISA correctly identified 97.8% (43 of 44) of samples as dengue positive while the Pan Bio Duo ELISA and Pan Bio RIT identified 95.45% (42 of 44). The sensitivities of both Pan Bio Duo ELISA and Pan Bio RIT for primary dengue and secondary dengue were 100% and 93.54% respectively. The specificity of three assays were MRL IgM ELISA 100%, Pan Bio Duo ELISA 92.8% and Pan Bio RIT 85.7%.


Asunto(s)
Dengue/diagnóstico , Virus del Dengue/inmunología , Brotes de Enfermedades , Humanos , India/epidemiología , Juego de Reactivos para Diagnóstico/normas , Sensibilidad y Especificidad
7.
Artículo en Inglés | IMSEAR | ID: sea-16359

RESUMEN

BACKGROUND & OBJECTIVES: HIV-1 gp160 is an important structural protein for the virus cell interaction and virus entry. Therefore, it is regarded as the most important target for HIV-1 vaccine development. In this study we investigated the use of HIV-1 gp160-DNA construct in eliciting specific and cross reactive cell mediated immune response in mice. METHODS: DNA segment encoding env, tat and rev genes of HIV-1 subtype B (strain BRU-2) was amplified and cloned into mammalian expression vector pCI to generate plasmid pCIBRU-TRE. Mice were injected intramuscularly four times at biweekly intervals with 100 micrograms/dose of pCIBRU-TRE in normal saline, and subsequently analysed for anti HIV-envelope (env) immune responses. RESULTS: A low antibody level was detected as determined by ELISA after 4 doses. Subsequent inoculations failed to increase the antibody titres significantly. Spleen cells from the immunized mice were used for the detection of cellular immune response by lymphocyte proliferation assays (LPA), in vitro production of cytokines and cytotoxic T lymphocyte (CTL) assays. T cell response which was seen from the second week onwards, persisted even at the end of 24 wk following the last dose. Similar levels of T cell proliferation were observed on stimulation with either homologous or heterologous peptides. Cytokine studies showed a Th1 type of response. A cross clade MHC class I restricted CTL response was observed against target cells stimulated with either homologous or heterologous HIV antigens. INTERPRETATION & CONCLUSION: This study demonstrated that DNA encoding full length HIV-1 env glycoprotein gp160 induces specific as well as cross reactive cell mediated immune responses in mice. However, the induction of antibody response was poor.


Asunto(s)
Vacunas contra el SIDA/genética , Animales , Reacciones Cruzadas , Anticuerpos Anti-VIH/biosíntesis , Proteínas gp160 de Envoltorio del VIH/genética , VIH-1/genética , Inmunidad Celular , Inmunización , Ratones , Ratones Endogámicos BALB C , Vacunas de ADN/genética
8.
Southeast Asian J Trop Med Public Health ; 2001 Jun; 32(2): 326-35
Artículo en Inglés | IMSEAR | ID: sea-35424

RESUMEN

The recent emergence of dengue hemorrhagic fever/dengue shock syndrome (DHF/ DSS) in India has been a source of concern. In the present study a quantitative comparison of 406 nucleotide long sequence from the capsid-premembrane junction region (C-PrM) of 9 dengue virus type 2 (DEN-2) isolates from Delhi with 10 DEN-2 isolates from diverse geographic areas provided sufficient information for estimating genetic relationships. The data indicated that the 1996 epidemic of DHF in Delhi was caused by genotype IV strains of DEN-2. This genotype, perhaps, displaced genotype V strains of DEN-2, which was circulating genotype in 1967. The period during which this displacement had occurred is not clear from the present study. Nonetheless, similar experience in four countries in Latin America and in Sri Lanka suggest that the introduction of new genotypes of DEN-2 displacing the circulating genotype may be associated with the appearance of DHF/DSS. More work is required to elucidate this hypothesis. Transitions at nucleotide positions 406 and 431 resulted in amino acid substitutions near (aa position 104, methionine --> valine) and at the hinge region (aa position 112, valine --> alanine) of C-PrM, respectively in all/most genotypes of group III and IV DEN-2 viruses analysed. Most of these virus strains have been isolated from DHF/DSS outbreaks. Significance of this observation is discussed. The data presented in this study suggest the utility of C-PrM sequence analysis for molecular epidemiology of dengue viruses.


Asunto(s)
Adolescente , Adulto , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Cápside/genética , Línea Celular , Niño , Cartilla de ADN , ADN Complementario/genética , Dengue/diagnóstico , Virus del Dengue/clasificación , Evolución Molecular , Femenino , Humanos , India , Masculino , Ratones , Persona de Mediana Edad , Datos de Secuencia Molecular , Filogenia , Homología de Secuencia de Aminoácido
9.
Indian J Med Sci ; 2001 Mar; 55(3): 149-56
Artículo en Inglés | IMSEAR | ID: sea-67546

RESUMEN

The aims of the present study were to carry out surveillance for dengue virus infection in adults with short-duration fever, and serological study of dengue virus infection in persons without fever. Patients were divided into two groups. Group 1 included patients above 12 years of age with fever of 2-12 days duration without any apparent cause. Of these, patients who presented with fever for 2-5 days were included for virus isolation (group 1a) while those who presented within 6-12 days of the onset of fever were included for the dengue-specific IgM serology (group 1b). Group 2 included a sample of population belonging to all age groups but without pyrexia and blood was collected for dengue-specific IgG serology. Twenty-six patients were enrolled in group 1a over a period of 4 months (September to December, 1997). Of these, DEN1 was isolated in 5 cases. Group 1b included 182 patients, out of which 34 (18.68%) were positive for dengue-specific IgM antibodies. Significantly, all the positive cases were detected during the months of September to November. Retro-orbital pain was present in a significantly more number of IgM-positive cases as compared to IgM-negative cases. Group 2 included 125 cases without fever. The overall positivity for dengue-specific IgG antibodies was 77.6%, with the highest positivity of 100% in the age group of 31-40 years. It was concluded that dengue virus infection is endemic in and around Delhi with peak incidence between September and Novemver. The prevalent serotype during September and December 1997 was DEN1. Since previous epidemic of DHF was due to DEN2 type, isolation of DEN1 serotype indicates changes of another epidemic of DHF due to DEN1 serotype. The stresses the urgent need for implementation of measures to control the transmission of dengue infection.


Asunto(s)
Adulto , Dengue/epidemiología , Dengue Grave/epidemiología , Países en Desarrollo , Femenino , Humanos , India/epidemiología , Masculino , Vigilancia de la Población , Estudios Seroepidemiológicos , Población Urbana/estadística & datos numéricos
10.
Indian J Ophthalmol ; 2000 Dec; 48(4): 307-9
Artículo en Inglés | IMSEAR | ID: sea-70505

RESUMEN

BACKGROUND: Platelets are an elective site for oxidative stress owing to their high content of polyunsaturated fatty acid. Increased lipid peroxidation and elevated platelet thiobarbituric acid-reacting substances (TBARS) signal oxidative stress. This possibly leads to retinal neovascularization in Eales' disease. METHODS: TBARS levels were estimated in consecutive cases of Eales' disease with neovascularisation (n = 26), Eales' disease without neovascularisation (n = 17) and healthy controls (n = 17). RESULTS: Platelet TBARS levels in the cases of Eales' disease with neovascularisation, Eales' disease without neovascularisation, and healthy controls were 0.66 +/- 0.1, 0.57 +/- 0.11 and 0.42 +/- 0.14 n moles TBARS formed/hour/10(8) platelets respectively. Student's t-test showed a significant increase in platelet TBARS levels in cases with neovascularisation as compared to cases without neovascularization (p < 0.05) and healthy controls (p < 0.01). CONCLUSION: The increase in platelet TBARS levels in proliferative Eales' disease is consistent with an emerging view that lipid peroxides may be associated with retinal neovascularisation.


Asunto(s)
Adolescente , Adulto , Biomarcadores/sangre , Plaquetas/metabolismo , Progresión de la Enfermedad , Humanos , Peroxidación de Lípido/fisiología , Peróxidos Lipídicos/sangre , Enfermedades de la Retina/sangre , Neovascularización Retiniana/sangre , Sustancias Reactivas al Ácido Tiobarbitúrico/metabolismo , Vasculitis/sangre
11.
Artículo en Inglés | IMSEAR | ID: sea-64408

RESUMEN

Budd-Chiari syndrome presents with ascites, edema and bleeding from esophageal varices. Presentation as bleeding scrotal varices is rare. We report a patient with Budd-Chiari syndrome who presented with recurrent bleeding from scrotal varices for 20 years.


Asunto(s)
Síndrome de Budd-Chiari/diagnóstico , Diagnóstico Diferencial , Estudios de Seguimiento , Hemorragia/diagnóstico , Humanos , Masculino , Persona de Mediana Edad , Escroto/irrigación sanguínea , Várices/diagnóstico
13.
Indian J Chest Dis Allied Sci ; 2000 Jul-Sep; 42(3): 157-60
Artículo en Inglés | IMSEAR | ID: sea-30168

RESUMEN

We report HIV seropositivity among tuberculosis patients from our hospital between 1994-99. Of the 500 patients with various forms of tuberculosis, two were found to be seropositive (0.4%). This report contrasts with the HIV seropositivity reports from other parts of India where increasing HIV seropositivity has been reported. As the HIV infection is making rapid in-roads in India, it is suggested that continuous HIV sero-surveillance should be done in patients with tuberculosis.


Asunto(s)
Adulto , Femenino , Infecciones por VIH/epidemiología , Seropositividad para VIH/epidemiología , Humanos , Incidencia , India/epidemiología , Masculino , Pruebas Serológicas , Tuberculosis Pulmonar/complicaciones
14.
Southeast Asian J Trop Med Public Health ; 1999 Sep; 30(3): 507-10
Artículo en Inglés | IMSEAR | ID: sea-32233

RESUMEN

Dengue fever (DF) and dengue hemorrhagic fever (DHF) are major public health problems in India. During the period following an epidemic, a study was carried out using virological and serological tests for confirmation of suspected cases of dengue virus infection in fever cases presenting to the All India Institute of Medical Sciences. Serum samples of suspected DF/DHF cases were processed from January to December 1997. In 37 samples from patients with fever of less than 5-day duration, received on ice, virus isolation was attempted in C6/36 clone of Aedes albopictus cell line, followed by indirect fluorescent antibody staining with monoclonal antibodies to dengue viruses 1 to 4. One hundred and forty-three serum samples from patients with more than 5 days fever were tested for dengue specific IgM antibody by either MAC-ELISA or a rapid immunochromatographic assay. Dengue virus type 1 was demonstrated by culture in 8 (21.6%) of 37 serum samples and IgM antibody could be detected in 42 (29.4%) of the 143 serum samples by the serological methods. The peak of dengue virus infection was seen from September to November 1997.


Asunto(s)
Adolescente , Adulto , Aedes , Animales , Anticuerpos Monoclonales , Niño , Preescolar , Dengue/sangre , Virus del Dengue/aislamiento & purificación , Ensayo de Inmunoadsorción Enzimática , Técnica del Anticuerpo Fluorescente Indirecta , Humanos , Inmunoglobulina G/sangre , Inmunoglobulina M/sangre , India/epidemiología , Lactante , Estaciones del Año
15.
Yonsei Medical Journal ; : 118-123, 1999.
Artículo en Inglés | WPRIM | ID: wpr-45264

RESUMEN

Transforming growth factor-beta (TGF-beta), a pleiotropic growth factor, is a potent inhibitor of cellular proliferation in cells of epithelial origin. Recently, it has been suggested that a loss of sensitivity to TGF-beta through a loss of expression of TGF-beta receptors T beta R-I and T beta R-II--is associated with tumor initiation and progression. Therefore, to investigate the relationship between TGF-beta receptors expression and carcinogenesis of bladder TCC, this study examined the expression of T beta R-I and T beta R-II in 46 bladder TCC patients using immunohistochemistry. Since histopathological grade is a widely accepted marker of prognosis, the results were compared in relation to the three grades of bladder TCC. The results demonstrated that the loss of TGF-beta receptors expression is associated with increasing histopathological grades of bladder TCC. Specifically, both T beta R-I and T beta R-II were readily detected in all 10 normal bladder mucosa specimens. Likewise, all 6 specimens of grade I TCC samples expressed high levels of both TGF-beta receptors. However, among grade II TCC samples, T beta R-I and T beta R-II were detected in 78% and 89%, respectively: among grade III TCC samples, T beta R-I and T beta R-II were detected in 45% and 41%, respectively. These results suggested that loss of sensitivity to TGF-beta may play a role in the progression of TCC from low to high grade disease.


Asunto(s)
Adulto , Anciano , Humanos , Neoplasias de la Vejiga Urinaria/patología , Neoplasias de la Vejiga Urinaria/metabolismo , Carcinoma de Células Transicionales/patología , Carcinoma de Células Transicionales/metabolismo , Persona de Mediana Edad , Receptores de Factores de Crecimiento Transformadores beta/metabolismo , Valores de Referencia
17.
Indian J Chest Dis Allied Sci ; 1998 Jan-Mar; 40(1): 5-16
Artículo en Inglés | IMSEAR | ID: sea-29230

RESUMEN

Polymerase chain reaction (PCR) has been found to be a sensitive and rapid method to confirm a clinical diagnosis of tuberculosis. We evaluated PCR for M. tuberculosis complex specific MPB64 gene for the diagnosis of pulmonary tuberculosis, in a double blind study. One hundred and eighty-two clinical samples (sputum, bronchioalveolar lavage and pleural fluid) from patients with a clinical diagnosis of pulmonary tuberculosis and 72 samples from patients with non-tubercular pulmonary lesions and normal healthy individuals were included. The samples were coded and clinical details were concealed from the laboratory, where conventional diagnostic methods and PCR were carried out independent of each other. On decoding and analysing the data, PCR was positive in 59% of single sputum samples from clinically diagnosed pulmonary tuberculosis, while M. tuberculosis could be grown in 18% of the samples. PCR could identify M. tuberculosis in 81.8% of the culture positive sputum samples. PCR was also positive in 71.4% of bronchioalveolar lavage (BAL) fluid and 60.7% pleural fluid samples from clinically suspected cases, which were mostly culture negative. On comparison with response to treatment, PCR was positive in 79.5% of patients who improved on anti-tuberculosis treatment, with a positive predictive value of 92%. PCR for MPB64 gene provides a useful alternative for the diagnosis of pulmonary tuberculosis from sputum and paucibacillary samples like BAL and pleural fluid in which conventional methods show low sensitivity, especially in areas from which strains show a low copy number of other PCR targets like the IS 6110 insertion sequence.


Asunto(s)
Líquido del Lavado Bronquioalveolar/microbiología , ADN Bacteriano/análisis , Amplificación de Genes , Genes Bacterianos , Humanos , Mycobacterium tuberculosis/genética , Derrame Pleural/microbiología , Reacción en Cadena de la Polimerasa/métodos , Valor Predictivo de las Pruebas , Reproducibilidad de los Resultados , Esputo/microbiología , Tuberculosis Pulmonar/diagnóstico
18.
Artículo en Inglés | IMSEAR | ID: sea-16151

RESUMEN

A rapid and simple nonradioisotopic method has been developed for detection of polymerase chain reaction (PCR) amplified product. Digoxigenin-11-dUTP (DIG-11-dUTP) was incorporated in the amplified product by including it in the PCR reaction mixture. The PCR product was detected colorimetrically either directly or by reverse phase hybridization method where an unlabelled oligo-nucleotide probe was immobilized on a nitrocellulose dipstick and the digoxigenin labelled PCR product was in the liquid phase. With this system the PCR product could be detected even after 10 cycles of amplification by both direct and hybridization methods. The method was applied on the amplified product of DNA from peripheral blood mononuclear cells from 10 HIV-1 ELISA positive and 8 ELISA negative individuals. PCR was positive in all ELISA positive, Western blot positive individuals from whom HIV-1 was also isolated. PCR was negative in all ELISA negative individuals.


Asunto(s)
Secuencia de Bases , Cartilla de ADN , ADN Viral/análisis , Ensayo de Inmunoadsorción Enzimática , Anticuerpos Anti-VIH/análisis , VIH-1/genética , Humanos , Datos de Secuencia Molecular , Hibridación de Ácido Nucleico , Reacción en Cadena de la Polimerasa/métodos
19.
Artículo en Inglés | IMSEAR | ID: sea-20353

RESUMEN

Anti-idiotypic antibodies (Ab-2) to HSV-1 (herpes simplex virus 1) neutralizing monoclonal antibody were raised by hybridoma. These Ab-2 were found to represent an epitope of glycoprotein B (gB-1) of this virus. To further characterise this antibody for its ability to mimic the antigenic epitope, in vitro lymphoproliferation assays were done. In this assay (i) antigen specific lymphocyte priming activity of the three monoclonal Ab-2 and (ii) the in vitro stimulating ability of these Ab-2 for gb-1 primed mouse lymphocytes were tested. We could identify two monoclonal Ab-2 which were able to prime the mouse lymphocytes in vivo. These antibodies were able to recognise the in vitro stimulation signal of the antigen gB-1 and consequently could proliferate. The stimulation index was comparable to that with the antigen. These two Ab-2 were also recognized by the antigen primed mouse lymphocytes in a specific manner.


Asunto(s)
Animales , Anticuerpos Antiidiotipos/inmunología , Anticuerpos Monoclonales/inmunología , Anticuerpos Antivirales/inmunología , Epítopos , Herpesvirus Humano 1/inmunología , Hibridomas , Activación de Linfocitos , Ratones , Pruebas de Neutralización
20.
Artículo en Inglés | IMSEAR | ID: sea-21289

RESUMEN

Fifty one biopsies from women with malignant lesions of the uterine cervix and 9 biopsies fron non-malignant lesions were examined for the presence of HPV 16 and 18 DNA sequences by in situ hybridization method using 35S-labelled DNA probes. HPV 16 DNA sequences were detected in 82.4 per cent biopsies from women with malignant lesions, whereas HPV 18 DNA was detected in only 3 biopsies which were also positive for HPV 16 DNA. Two biopsies from non-malignant lesions were positive for HPV 16 DNA only. Data were also analysed according to the histologic type of cancer. It was observed that no significant correlation existed between HPV types and different histologic types of cervical cancer.


Asunto(s)
Sondas de ADN de HPV , ADN Viral/análisis , Femenino , Humanos , Hibridación in Situ , India , Papillomaviridae/aislamiento & purificación , Infecciones Tumorales por Virus/diagnóstico , Enfermedades del Cuello del Útero/microbiología
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