RESUMEN
BACKGROUND/AIMS: Pancreatic cancer (PC) patients have poor prognoses because this cancer is typically diagnosed at an advanced stage and the therapeutic options are limited. We examined the potential of metabolic profiling for early diagnosis and identification of potential therapeutic targets. METHODS: Ten patients and 10 healthy volunteer controls older than 20 years of age were enrolled between May and December 2015. The patients were confirmed to have pancreatic ductal adenocarcinoma cytologically or histologically. Blood plasma samples were derivatized and analyzed by gas chromatography mass spectrometry (GC-MS). Untargeted GC-MS data were analyzed using statistical methods, including Wilcoxon rank-sum test and principal component analyses. RESULTS: L-lysine was 1.36-fold higher in patients than in healthy controls (p<0.05). L-leucine was 0.63-fold lower (p<0.01) and palmitic acid was 0.93-fold lower (p<0.5) in patients than in controls. Orthogonal partial least squared-discriminant analysis revealed significant differences between the patients and controls. CONCLUSIONS: This study suggests that the metabolic profiles of patients with PC are distinct from those of the healthy population. Further studies are required to develop methods for early diagnosis and identify therapeutic targets.
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Humanos , Adenocarcinoma , Diagnóstico Precoz , Cromatografía de Gases y Espectrometría de Masas , Voluntarios Sanos , Corea (Geográfico) , Leucina , Lisina , Metaboloma , Ácido Palmítico , Conductos Pancreáticos , Neoplasias Pancreáticas , Plasma , Análisis de Componente Principal , PronósticoRESUMEN
Plasmodium vivax, a protozoan malaria parasite of humans, represents a major public health concern in the Republic of Korea (= South Korea). However, little is known about the genetic properties and population structures of the P. vivax isolates circulating in South Korea. This article reviews known polymorphic genetic markers in South Korean isolates of P. vivax and briefly summarizes the current issues surrounding the gene and population structures of this parasite. The critical genetic characteristics of major antigens of the parasite, such as circumsporozoite protein (CSP), merozoite surface protein 1 (MSP-1) and MSP-3, Duffy binding protein (DBP), apical membrane antigen 1 (AMA-1), and GAM-1, are also discussed.
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Animales , Humanos , Secuencia de Aminoácidos , Antígenos de Protozoos/química , Secuencia de Bases , Malaria Vivax/parasitología , Datos de Secuencia Molecular , Plasmodium vivax/química , Polimorfismo Genético , Proteínas Protozoarias/química , República de Corea , Alineación de SecuenciaRESUMEN
A complete cDNA sequence encoding a pore-forming subunit (Kir6.2) of ATP-senstive potassium channel in the adult worm, Clonorchis sinensis, termed CsKir6.2, was isolated from an adult cDNA library. The cDNA contained a single open-reading frame of 333 amino acids, which has a structural motif (a GFG-motif) of the putative pore-forming loop of the Kir6.2. Peculiarly, the CsKir6.2 shows a lack-sequence structure, which deleted 57 amino acids were deleted from its N-terminus. The predicted amino acid sequence revealed a highly conserved sequence as other known other Kir6.2 subunits. The mRNA was weekly expressed in the adult worm.
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Animales , Humanos , Adenosina Trifosfato/metabolismo , Secuencia de Aminoácidos , Secuencia de Bases , Clonación Molecular , Clonorchis sinensis/genética , Proteínas del Helminto/genética , Datos de Secuencia Molecular , Canales de Potasio de Rectificación Interna/genética , ARN de Helminto/química , Alineación de SecuenciaRESUMEN
Experimental animals were inhalated 50 ppm formaldehyde gas for 3 times with one hour exposure and one hour rest. The olfactory mucosa were taken from the animals on 4, 7, 9, 11 days and 2-6 weeks after the inhalation. The characteristics of the various glycoproteins and the mitotic activity of the olfactory epithelial cells were investigated using lectins and 5'-bromodeoxyuridine (BrdU, 50 mg/kg) which injected one hour before sacrifice of the animals. The results were as follows; 1. In experimental animals, the degenerative changes of the olfactory epithelium such as atrophy and squamous metaplasia were observed until 2 weeks after formaldehyde gas inhalation. 2. In control animals, positive reactions appeared in the supporting cell to PNA, SBA, WGA, ECL, PHA-L and in the olfactory cells to PNA, SBA, WGA, UEA and in the proper basal cell to GS-I, SBA, WGA, PHA-L. In experimental groups, the positive reaction was increased in the supporting cells to SBA, ECL, PHA-L and in Bowman's gland to used lectins except ECL, GS-I. 3. The number of BrdU labelled cells in the olfactory epithelium was 14.8+/-1.2/mm in the control animals. The mitotic activities were decreased to 4.8+/-0.8 mm in 2 weeks and recovered in 3 weeks after the gas inhalation. 4. To find the stem cells of olfactory receptor cells, double labelling method was performed with lectins which were specific for proper basal cells (GS-I or PHA-L) and BrdU immunohistochemistry. The ratio of globose/proper basal cells in BrdU labelled cells with GS-I lectin positive reaction was 82%/18% in control group, 39.3%/60.7% in lesion group and 55.5%/44.5% in recovery group.