RESUMEN
Abstract Micro-RNA-221(miR-221) is one of oncogenic miRNAs that plays a vital role in the development and progression of oral cancers. The aim of this study is to introduce a new gene therapy for oral squamous cell carcinoma by blocking the expression of oncogenic miR-221 by its inhibitor. The present work was performed on squamous cell carcinoma cell line SCC-25 and anti-miR-221 was delivered to the cells using an ultrasound micro bubbles. Assessment of the effect of miR-221 inhibitor on SCC-25 cells was done using MTT assay, cell cycle analysis and apoptosis detection. In addition, reverse transcription-polymerase chain reaction was also used to detect the expression -miR-221 and its target genes. Using ANOVA, statistical analysis of the results showed significant inhibition of cell viability with and induction of cell apoptosis of SCC-25 cell line after transfection. Moreover, the expression of miR-221, Epidermal growth factor receptor (EGFR) and CDKNIB/p27 were downregulated without significant difference. Transfection of SCC-25 by inhibitor of miR-221 resulting in blockage of its expression leading to arresting of tumor growth. These results proved the effective role of micro-RNA inhibitors as novel therapeutic agent for oral cancers.
Resumo Micro-RNA-221 (miR-221) é um dos miRNAs oncogênicos que desempenham um papel vital no desenvolvimento e progressão de carcinomas orais. O objetivo deste estudo é apresentar uma nova terapia gênica para o carcinoma epidermóide oral por meio do bloqueio da expressão do miR-221 oncogênico por seu inibidor. O presente trabalho foi realizado na linhagem de células de carcinoma de células escamosas SCC-25 e o anti-miR-221 foi administrado às células usando micro-bolhas de ultrassom. A avaliação do efeito do inibidor miR-221 em células SCC-25 foi feita usando ensaio de MTT, análise do ciclo celular e detecção de apoptose. Além disso, a reação em cadeia da polimerase com transcrição reversa também foi usada para detectar a expressão -miR-221 e seus genes-alvo. Usando ANOVA, a análise estatística dos resultados mostrou inibição significativa da viabilidade celular e indução da apoptose celular da linhagem celular SCC-25 após a transfecção. Além disso, a expressão de miR-221, receptor do fator de crescimento epidérmico (EGFR) e CDKNIB/p27 foram regulados para baixo sem diferença significativa. A transfecção de SCC-25 por inibidor de miR-221 resultou no bloqueio de sua expressão, levando à interrupção do crescimento do tumor. Esses resultados comprovaram o papel eficaz dos inibidores de micro-RNA como novo agente terapêutico para carcinomas orais.
Asunto(s)
Humanos , Neoplasias de la Boca/genética , Carcinoma de Células Escamosas/genética , Carcinoma de Células Escamosas/terapia , MicroARNs/antagonistas & inhibidores , MicroARNs/genética , MicroARNs/uso terapéutico , Neoplasias de la Boca/terapia , Terapia Genética , Apoptosis , Línea Celular Tumoral , Proliferación CelularRESUMEN
Background: evaluation of ovarian reserve has become an essential part of the treatment assessment of woman to undergo assisted reproductive technique
Aim of the Work: this work was conducted to measure FSH, LH and E2 at any day of the whole menstrual cycle compared to day2 with measurement and establishing an actual negative correlation between FSH and E2
Patients and methods: observational analytical prospective study on 50 women attending the Gynecology Outpatient Clinics of Maternity Hospital, Ain-Shams University in 2016
Results: there was a significant negative correlation between E2/FSH on the 3[rd], 5[th] and 10[th] days of the cycle. Also there was insignificant negative correlation on day 21
Conclusion: there was negative correlation between basal day2 E2/FSH which was equivalent or similar to that ratio on days [5, 10, 21] so there was no need to wait for hormonal analysis to the next cycle [day 2 or 3] to save time specially in patients >35years; for them there was importance of cycle day3 for evaluation of ovarian reserve and prior ovulation induction and subsequent pregnancy potential during the infertility work up
Recommendations: during the infertility work up, rigid adherence to cycle day3 collection, no longer seems necessary ,no need to wait for hormonal analysis to Estradiol and FSH to the next cycle as there was equivalent negative correlation between E2/FSH on menstrual cycle days [3,5,10,21] aiming for saving time