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@#Marfan syndrome (MFS) is an autosomal dominant and multisystemic disorder affecting the connective tissues. Patients with MFS may exhibit characteristic oral features including maxillary protrusion, high palate, crowded teeth, and fragility of the temporomandibular joint. Periodontal manifestations may include a higher prevalence of gingivitis and/or periodontitis owing to the high concentration of elastic fibres in the periodontal ligament. This case report describes the management of gingival enlargement associated with MFS. The patient was successfully managed with non-surgical and surgical periodontal therapy. In patients with oral manifestations of systemic disorders, patient motivation and effective treatment planning are able to achieve the optimum periodontal and oral health outcomes.
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Background & objectives: Regional Virus Research and Diagnostic Laboratory established at ICMR-National Institute of Cholera and Enteric Diseases (NICED) regularly receives samples for dengue screening and serotyping from patients of acute febrile illness (AFI) from Kolkata and adjacent districts. In this study, data over a three year period (August 2016-July 2019) was retrospectively analyzed to provide insight into the epidemiological trends of dengue fever in this region. Methods: Serological screening of dengue was performed by detection of NS1 antigen and/or immunoglobulin M (IgM) antibody. Dengue serotyping was done by conventional or real-time reverse transcriptase–PCR. The data were analyzed to describe the distribution of dengue with respect to age of patient, duration of fever on the day of blood collection and month of the year. Zip codes were used for spatial plotting. Results: Out of the 24,474 samples received from Kolkata and its adjacent districts (Hooghly, Howrah, North and South 24 Parganas), 38.3 per cent (95% confidence interval: 37.7-38.9%) samples were screened positive for dengue. The correlation between age and dengue positivity was found to be weak. A combination of dengue NS1 antigen and dengue IgM antibody detection may be a better option for detecting dengue positivity compared to a single test. Most AFI cases were tested from August to November during the study period, with maximum dengue positivity noted during September (45.9%). The predominant serotype of 2016, dengue virus serotype 1 (DENV-1), was almost entirely replaced by DENV-2 in 2017 and 2018. Interpretation & conclusions: Dengue continues to be an important cause of AFI in the region and round-the-year preventive measures are required for its control. Serotype switching is alarming and should be monitored routinely.
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In this study, we have collected and screened a total of 268 stool samples from diarrheal patients admitted to an Infectious disease hospital in Kolkata for the presence of Cryptosporidium spp. The initial diagnosis was carried out by microscopy followed by genus specific polymerase chain reaction assays based on 70 kDa heat shock proteins (HSP70). DNA sequencing of the amplified locus has been employed for determination of genetic diversity of the local isolates. Out of 268 collected samples, 12 (4.48%) were positive for Cryptosporidium spp. Sequences analysis of 70 kDa heat shock proteins locus in 12 Cryptosporidium local isolates revealed that 2.24% and 1.86% of samples were showing 99% to 100% identity with C. parvum and C. hominis. Along with the other 2 major species one recently described globally distributed pathogenic species Cryptosporidium viatorum has been identified. The HSP70 locus sequence of the isolate showed 100% similarity with a previously described isolate of C. viatorum (Accession No. JX978274.1, JX978273.1, and JN846706.1) present in GenBank.
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In this study, we have collected and screened a total of 268 stool samples from diarrheal patients admitted to an Infectious disease hospital in Kolkata for the presence of Cryptosporidium spp. The initial diagnosis was carried out by microscopy followed by genus specific polymerase chain reaction assays based on 70 kDa heat shock proteins (HSP70). DNA sequencing of the amplified locus has been employed for determination of genetic diversity of the local isolates. Out of 268 collected samples, 12 (4.48%) were positive for Cryptosporidium spp. Sequences analysis of 70 kDa heat shock proteins locus in 12 Cryptosporidium local isolates revealed that 2.24% and 1.86% of samples were showing 99% to 100% identity with C. parvum and C. hominis. Along with the other 2 major species one recently described globally distributed pathogenic species Cryptosporidium viatorum has been identified. The HSP70 locus sequence of the isolate showed 100% similarity with a previously described isolate of C. viatorum (Accession No. JX978274.1, JX978273.1, and JN846706.1) present in GenBank.
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Background: Ghr¸ta murcchana _x005F_x005F_x0001_ is a process of pre-treatment recommended in Ayurveda to purify gheebefore it can be used for siddha ghr¸ta which is claimed to improve the properties of the ghee in generaland that of the prepared siddha ghr¸ta.Objective: This work is aimed at studying the physiochemical properties of ghee and murcchita ghr¸ _x005F_x005F_x0001_ ta inorder to understand the impact of ghr¸ta murcchana _x005F_x005F_x0001_ process.Materials and methods: Ghee and murcchita ghr¸ _x005F_x005F_x0001_ ta were prepared from the milk of local Pahadi, Jersey andHolstein cows. The samples were characterized by FTIR spectroscopy, differential scanning calorimetryand free fatty acid measurements.Results: Among the samples studied, the Holstein cow ghee was found to contain the least amount offree acid (1.34%) whereas ghr¸ta murcchana _x005F_x005F_x0001_ process led to further decrease in the free acid contentpolymorphism was observed in the samples as evidenced by multiple melting points. In most cases,murcchita ghr¸ _x005F_x005F_x0001_ ta was found to contain less solid fat than the corresponding ghee implying that the highmelting compound was converted to low melting one during the process.Conclusion: The observed lowering of free fatty acid and solid fat contents in the ghee samples mayprovide a possible validation to the performance enhancement of the ghr¸ta murcchana _x005F_x005F_x0001_ process.
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Direct massively parallel sequencing of SARS-CoV-2 genome was undertaken from nasopharyngeal andoropharyngeal swab samples of infected individuals in Eastern India. Seven of the isolates belonged to the A2aclade, while one belonged to the B4 clade. Specific mutations, characteristic of the A2a clade, were alsodetected, which included the P323L in RNA-dependent RNA polymerase and D614G in the Spike glycoprotein. Further, our data revealed emergence of novel subclones harbouring nonsynonymous mutations, viz.G1124V in Spike (S) protein, R203K, and G204R in the nucleocapsid (N) protein. The N protein mutationsreside in the SR-rich region involved in viral capsid formation and the S protein mutation is in the S2 domain,which is involved in triggering viral fusion with the host cell membrane. Interesting correlation was observedbetween these mutations and travel or contact history of COVID-19 positive cases. Consequent alterations ofmiRNA binding and structure were also predicted for these mutations. More importantly, the possibleimplications of mutation D614G (in SD domain) and G1124V (in S2 subunit) on the structural stability of Sprotein have also been discussed. Results report for the first time a bird’s eye view on the accumulation ofmutations in SARS-CoV-2 genome in Eastern India.
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ObjectivesThis study aimed to identify virulent and antimicrobial resistant genes in fecal E. coli in Mbouda, Cameroon.MethodsA total of 599 fecal samples were collected from patients with enteric infections who were ≥ 20 years old. E. coli was isolated on the MacConkey agar and virulent genes were detected by multiplex/simplex PCR. Isolates in which ≥ 1 virulent gene was detected were subjected to antibiotic susceptibility testing. The resulting resistant isolates were subjected to PCR, followed by sequencing for resistant genes detection.ResultsThere were 119 enterovirulent E. coli identified, amongst which 47.05% were atypical enteropathogenic E. coli (EPEC), 36.97% enterotoxigenic E. coli, 10.08% Shiga toxin producing E. coli (STEC) and 5.88% were enteroinvasive E. coli (EIEC). The occurrence of the eae gene (47.06%) was higher compared with CVD432 (33.61%), aaic (13.45%), stx2 (10.08%) and stx1 (0.84%). High resistance rates were noted for ampicillin (94.64% EPEC, 91.67% STEC, 59.09% EAEC, and 57.14% EIEC) and sulfamethoxazole-trimethoprim (100% EPEC and 83.33% STEC, 81.82% EAEC and 71.43% EIEC). sul2 (71.43%), tetB (64.71%), tetA (59.94%) and blaTEM (52.10%) were detected. A double mutation (S83L; D87N) was seen in gyrA and a single mutation (S80I) was observed in parC.ConclusionThese findings suggested that measures should be taken to reduce the harm of E. coli to public health.
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Background: Janani Suraksha Yojana (JSY) replaces the National Maternity Benefit Scheme. It was launched by the Government of India in April 2005. The aim was to reduce maternal and neonatal mortality by increasing institutional delivery by providing cash incentive to the beneficiaries as well as the link worker ASHA. Objectives of this study was to assess receipt of Janani Suraksha Yojana (JSY) cash incentive and to assess fields of utilization of Janani Suraksha Yojana (JSY) cash incentive.Methods: A Community based cross-sectional study was conducted among 384 mothers delivered within last one year in urban slum of Raipur city. Study centre was department of community medicine, Pt. J.N.M. medical college Raipur, Chhattisgarh. A pre-designed and pre-tested questionnaire was used to interview the study subjects.Results: Out of total 384 study subject’s cheque of JSY cash incentive was received by 70.83%. All (100%) beneficiaries who received cheque was stipulated amount as per JSY guideline. Reasons for not receiving cheque was no availability of BPL card for those who delivered in accredited private health facility. Other reasons were absconded after delivery, unaware of cash incentive, did not go back to get the cheque when called later, patient shifted to another ward. None of the study subjects who delivered at home received the cash incentive cheque. Realization of cheque was not done by 22.79% of study subjects due to complex procedure to open an account in bank. Cash incentive money was utilized in neonatal care, drugs, food, household activities and some deposited in bank.Conclusions: JSY scheme is definitely functioning well in terms of providing cash incentive cheque of JSY on delivery in Government Health facility. There is need to simplify the procedure to open an account so that beneficiaries can avail cash incentive money. There is need to address the problems to receive cheque in home delivery and accredited Private health facility in addition to motivation for institutional delivery.
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Background: The prevalence of paediatric skin diseases varies worldwide. Paediatric dermatoses require a separate view from adult dermatoses as there are important differences in clinical presentation, treatment and prognosis. Their characterisation is essential for the preparation of academic, research and health plans. Aim: The study was carried out to find the incidence of skin diseases in children under 15 years of age. Methods: All children below 15 years of age attending dermatology outpatient department for first time were evaluated for various skin dermatoses. Skin diseases were classified into groups like infections and infestations, eczematous, non-infective dermatoses and hypersensitivity reactions. Results: Male children predominated with male female ratio 1.3:1. Infections and infestations were the most common (41.8%) cause of skin dermatoses followed by dermatitis (20.7%), non-infective & autoimmune dermatoses (13.4%) and hypersensitivity reactions seen in 10.8% patients. Miliaria and other physical dermatoses contributed to about 6% of the total cases. Conclusion: Majority of paediatric dermatoses can be grouped into 8 categories. Significant proportion of dermatoses are infectious and eczematous types. Infections are more prevalent in low socio-economic strata and with proper education and creating awareness they can be minimised to a significant extent.
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Drug induced Lupus Erythematosus (DILE) is a form of rare adverse drug reaction caused due to many drugs, including Isoniazid. Diagnosis is made on the basis of a temporal relationship between the drug administered and the onset of symptoms and on the basis of the antibody profile. We describe the case of a 13 year old female who was diagnosed as having abdominal tuberculosis and was put on ATT and later presented with signs and symptoms of Lupus Erythematosus, with a history of photosensitivity, oral ulcers, and malar rash. Keywords: Isoniazid, Tuberculosis, Drug Induced Lupus
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Background: Superficial fungal infection are most common fungal infection affecting 20 -25% of population living in tropical and subtropical countries. Global warming, rapid industrialization, migration of population and changing lifestyle are contributory factors. Aim: To study the host factors responsible for Tinea cruris infection and clinical resistance to treatment. Methods: Hundred patients of tinea cruris were taken for study and data regarding age, occupation, income, education and prior treatment taken was collected. Modified Kupuswamy scale 2017 was applied for socioeconomic status. Results: ‘Majority of patients were males (85%). Young adults (20-40 years) with low socioeconomic status were predominantly affected. Application of steroids and poor compliance to treatment were contributory factors in development of clinical resistance to treatment. Conclusion: By improving working environment and socioeconomic status, mass awareness, proper counseling we can restrain the ongoing epidemic of dermatophytes infection.
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Programmed cell death protein 1 (PD-1) is an immuno-inhibitory cell surface receptor protein of the myeloid, and lymphoid cell. PD-L1 is the ligand of PD-1, which is abundant in different malignant tissue e.g. skin, colon and breast cancer. PD-1/PD-L1 interaction helps the tumour cell to escape from the immune response by limiting TCR mediated T lymphocytes proliferation. Recently, PD-1 or PD-L1 blocking immunotherapy proved their efficacy in the treatment of different cancers. However, PD-1/PD-L1 interaction is well studied in T lymphocytes, but little is known about its function in tumour-associated macrophages (TAMs). In the tumour microenvironment, phagocytosis by TAMs plays a vital role in the immune response. In this review, the significance of PD-1 expression by TAMs and how it influences tumour immunity will be discussed. Recently, it has been found that PD-1 can express by TAMs and its expression level is directly related to duration and stages of colon cancer. TAMs expression of PD-1 was shown to be related to significant depletion of cancer cell phagocytosis. Monoclonal antibody against either PD-1 or PD-L1 in mice model of colon cancer promotes tumour cell phagocytosis by TAMs, thereby limiting the growth of the tumour and increase life expectancy. Therefore, PD-1 can be a promising target in macrophage-mediated immune therapy.
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Animales , Ratones , Neoplasias de la Mama , Muerte Celular , Colon , Neoplasias del Colon , Citofagocitosis , Inmunoterapia , Esperanza de Vida , Linfocitos , Macrófagos , Fagocitosis , Piel , Linfocitos T , Naciones UnidasRESUMEN
The hila and adjacent pre-hilar parts of 72 kidneys (35 right and 37 left) isolated from formalin fixed cadaverswere examined. The hilum and prehilar area of each kidney was dissected to observe the total number ofbranches of the main renal artery. This information is useful in nephron sparing surgeries such as partialnephrectomy by laparoscopic approach. Subsequently, prehilar branching of the main renal artery before itenters the hilum was observed in 48.6% of kidneys with a higher incidence of 51.3% in left kidneys as comparedwith the incidence of the right side (45.7%). We further observe the variations of branching patterns in the mainrenal artery and calculate the incidences. Finally, our results are compared with that of similar studies conductedpreviously.
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Giardia lamblia, an anaerobic, amitochondriate protozoan parasite causes parasitic infection giardiasis in children and young adults. It produces pyruvate, a major metabolic product for its fermentative metabolism. The current study was undertaken to explore the effects of pyruvate as a physiological antioxidant during oxidative stress in Giardia by cysteine-ascorbate deprivation and further investigation upon the hypothesis that oxidative stress due to metabolism was the reason behind the cytotoxicity. We have estimated intracellular reactive oxygen species generation due to cysteine-ascorbate deprivation in Giardia. In the present study, we have examined the effects of extracellular addition of pyruvate, during oxidative stress generated from cysteine-ascorbate deprivation in culture media on DNA damage in Giardia. The intracellular pyruvate concentrations at several time points were measured in the trophozoites during stress. Trophozoites viability under cysteine-ascorbate deprived (CAD) medium in presence and absence of extracellular pyruvate has also been measured. The exogenous addition of a physiologically relevant concentration of pyruvate to trophozoites suspension was shown to attenuate the rate of ROS generation. We have demonstrated that Giardia protects itself from destructive consequences of ROS by maintaining the intracellular pyruvate concentration. Pyruvate recovers Giardia trophozoites from oxidative stress by decreasing the number of DNA breaks that might favor DNA repair.
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Niño , Humanos , Adulto Joven , Medios de Cultivo , Roturas del ADN , Daño del ADN , Reparación del ADN , Giardia lamblia , Giardia , Giardiasis , Metabolismo , Estrés Oxidativo , Parásitos , Ácido Pirúvico , Especies Reactivas de Oxígeno , TrofozoítosRESUMEN
@#Exposure to groundwater arsenic contamination has been demonstrated to be associated with an increased risk of diabetes mellitus in Bangladesh. But, the association between arsenic exposure and Gestational Diabetes Mellitus (GDM) in Bangladesh remains to be reported. This cross-sectional study was carried out to assess the occurrence of GDM amongst pregnant women from the arsenic contaminated area. A total of 200 pregnant women who were aged 20 years and more, having gestational age more than 21 weeks, and had attended a district hospital for antenatal checkup were selected for the study. Of the total 200 participants, 15.5% were found to have GDM and it was significantly high (p=0.029) in women with higher gestational age. Regarding arsenic exposure, 39.5% of the participants were categorized as arsenic exposed and amongst them the proportion of GDM was found high (20.3%). Binary logistic regression analysis showed that the increase in gestational age and BMI, and a higher level of arsenic in urine of the total participants, had significantly (p<0.05) ability to predict the likelihood of having GDM (1.2, 1.1 and 9.2 times respectively). While logistic regression analysis among the arsenic exposed participants showed that higher level of BMI was 1.2 times and arsenic in urine was 9.4 times likely to predict the likelihood of having GDM. Thus, it was revealed that the increased concentration of arsenic in urine had the strongest ability to predict the likelihood of developing GDM among the pregnant women. The study concluded that the arsenic exposed pregnant women suffered more from GDM compared to the non-exposed pregnant woman.
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@#Arsenicosis, the illness due to chronic arsenic toxicity is prevalent in both Nepal and Bangladesh. The occurrence of arsenicosis depends upon many factors including food and nutrition. The objective of this study was to find out any difference of food habits among the arsenic exposed households of both countries and the relationship with the occurrence of arsenicosis. This was a cross-sectional comparative study, conducted among the arsenic exposed rural households of Nawalparasi district in Nepal and Faridpur district in Bangladesh. A total of 190 and 200 female rural households from Nepal and Bangladesh were selected respectively as the respondents. The majority of the respondents of both countries were under the age of 40 years. The prevalence of arsenicosis was found significantly low (χ2 = 8.847; p=.002) among the Nepalese households (7.3%) than that of Bangladeshi households (11.0%). As a staple food, rice, vegetables and pulses were more common among the Nepalese households in comparison to that of Bangladesh (χ2=5.739; p=.017). In addition to staple food Nepalese households were found to take significantly more (p<.05) bread (74.7%), egg (73.2%), milk (68.9%) and fruits (58.4%). In contrast, Bangladeshi households took a little more meat (59.0%) and fish (73.5%). To get arsenic-safe water, 39.5% Bangladeshi households used a filter while a few Nepalese households (2.6%) used that. Nepalese households were found to take more protein and vitamins rich foods as staple food compared to that of Bangladeshi households, which might play a role in the low occurrence of arsenicosis amongst them.
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Arsénico , Intoxicación por Arsénico , Alimentos , Ciencias de la Nutrición , Conducta AlimentariaRESUMEN
Arsenicosis, the illness due to chronic arsenic toxicity is prevalent in both Nepal and Bangladesh. The occurrence of arsenicosis depends upon many factors including food and nutrition. The objective of this study was to find out any difference of food habits among the arsenic exposed households of both countries and the relationship with the occurrence of arsenicosis. This was a cross-sectional comparative study, conducted among the arsenic exposed rural households of Nawalparasi district in Nepal and Faridpur district in Bangladesh. A total of 190 and 200 female rural households from Nepal and Bangladesh were selected respectively as the respondents. The majority of the respondents of both countries were under the age of 40 years. The prevalence of arsenicosis was found significantly low (χ2 = 8.847; p=.002) among the Nepalese households (7.3%) than that of Bangladeshi households (11.0%). As a staple food, rice, vegetables and pulses were more common among the Nepalese households in comparison to that of Bangladesh (χ2=5.739; p=.017). In addition to staple food Nepalese households were found to take significantly more (p<.05) bread (74.7%), egg (73.2%), milk (68.9%) and fruits (58.4%). In contrast, Bangladeshi households took a little more meat (59.0%) and fish (73.5%). To get arsenic-safe water, 39.5% Bangladeshi households used a filter while a few Nepalese households (2.6%) used that. Nepalese households were found to take more protein and vitamins rich foods as staple food compared to that of Bangladeshi households, which might play a role in the low occurrence of arsenicosis amongst them.
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Humanos , Organismos Acuáticos/aislamiento & purificación , Técnicas de Tipificación Bacteriana/métodos , Ácidos Grasos/biosíntesis , Pseudomonas aeruginosa/genética , Pseudomonas aeruginosa/aislamiento & purificación , Organismos Acuáticos/genética , Genotipo , Geografía , Variación Genética/genética , India , Infecciones por Pseudomonas/microbiologíaRESUMEN
Background: The two major changes that may occur in elderly individual without neurologic deficits is enlargement of ventricles and cortical atrophy. Aim of the study was to statistically analyse the dimensions of Fourth ventricle in humans and also to study the Changes that occur during ageing. Ventricular size of males and females was compared. METHOD: The CT images of 112 adult individuals (Age Group 21-60) and 88 ageing individuals (Age above 61) was studied in both males and females. Measurements like vertical length, height, anterior-posterior diameter and transverse diameter of fourth ventricle was made by using dicomworks software. RESULT: This study showed positive co-relation of age with dimensions of fourth ventricle and the dimensions of the fourth ventricle were enlarged with physiologic ageing. Also the dimensions of fourth ventricle were more in males as compared to females.