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1.
Acta Physiologica Sinica ; (6): 593-599, 2013.
Artículo en Chino | WPRIM | ID: wpr-297533

RESUMEN

This study was to observe the effect and possible mechanism of somatostatin analogue octreotide (OCT) on cross excitation of adjacent segment of spinal nerve in rat. Cutaneous branches of T9-T13 spinal dorsal rami were chosen and dissected free for the following recording and stimulation. Only single unit fiber was used for recording, and the adjacent segment of nerve stem was used for antidromic electrical stimulation. To investigate the change of discharge rate and mechanical threshold, OCT and (or) somatostatin receptor antagonist cyclo-somatostatin (c-SOM) were applied to the receptive field following the antidromic electrical stimulation. The result showed that injection of OCT inhibited the increase of discharge rate and the decrease of mechanical threshold induced by the electrical stimulation (cross excitation); c-SOM reversed the effects of OCT. Application of c-SOM alone enhanced the cross excitation effects. The results suggest local application of somatostatin analogue OCT can inhibit the cross excitation between the two segments of spinal nerve by somatostatin receptor.


Asunto(s)
Animales , Ratas , Estimulación Eléctrica , Octreótido , Farmacología , Péptidos Cíclicos , Farmacología , Receptores de Somatostatina , Fisiología , Somatostatina , Nervios Espinales
2.
Chinese Journal of Pediatrics ; (12): 522-525, 2008.
Artículo en Chino | WPRIM | ID: wpr-300744

RESUMEN

<p><b>OBJECTIVE</b>To establish a quick method to detect drug resistance of Mycoplasma pneumoniae (MP) and study the condition of drug resistance in MP infection.</p><p><b>METHODS</b>MP 23S rRNA target gene in throat swab specimens from 200 patients with suspected MP infection was detected by using nested PCR and DNA sequencing. The result of 23S rRNA gene detection was confirmed by MP isolation and drug susceptibility test in vitro for reliability.</p><p><b>RESULTS</b>Of the 200 clinical specimens, 64 were proved to be positive for MP through MP-IgM antibody, MP specific 16S rRNA nested PCR and MP isolation . The 23S rRNA gene was amplified and the gene sequence was compared with MP reference strain in Genbank, 26 were identical to the reference strain, 38 had a point mutation in 23S rRNA. Among them, 35 had A to G mutation at position 2063, 1 had A to C mutation at position 2063 and 2 had A to G mutation at position 2064, the percentage of drug resistance was 59.4%. The sensitivity of the gene detection method was 10(2) ccu/ml and it was confirmed to be reliable by MP isolation and drug susceptibility test.</p><p><b>CONCLUSIONS</b>The gene detection method could detect MP drug resistant gene directly from clinical specimen, which has the advantages of high specificity, high sensitivity and quickness. It is of great significance for diagnosis of MP infection because MP isolation is difficult and time-consuming.</p>


Asunto(s)
Adolescente , Niño , Preescolar , Femenino , Humanos , Lactante , Masculino , Farmacorresistencia Bacteriana , Genes de ARNr , Pruebas de Sensibilidad Microbiana , Mycoplasma pneumoniae , Genética , Mutación Puntual , Reacción en Cadena de la Polimerasa , ARN Bacteriano , Genética , ARN Ribosómico 23S , Genética
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