Role of bioinoculants and organic fertilizers in fodder production and quality of leguminous tree species.

The comparative effect of dual inoculation of native N fixer (Rhizobium) and AM fungi consortia with different organic fertilizers (vermicompost and farm yard manure) on fodder production and quality of two leguminous tree species (Leucaena leucocephala (Lam) de. Wit. and Sesbania sesban (L.) Merr.) in silvopastoral system and their impact on the fodder production of un-inoculated Panicum maximum Jacq. under cut and carry system. After three years of plantation maximum tree survival was in L. leucocephala in all the treatments in comparison to S. sesban while fodder production was more in S. sesban for initial two years and in third year it accelerated in L. leucocephala. Dual inoculation with vermicompost significantly improved fodder production, fodder quality and rhizosphere microflora in L. leucocephala but in S. sesban dual inoculation was at par with single inoculation of N fixer, AM fungi and control (without inoculation). The grass production was higher with L. leucocephala for two years while in third year it was more with S. sesban. The association of Rhizobium with AM fungi in L. leucocephala was better than in S. sesban.

Antioxidative response of Lemna polyrrhiza L. to cadmium stress.

Growth, lipid peroxidation, different antioxidative enzymes and metal accumulation were studied in Lemna polyrrhiza treated with different concentrations (1-40 ppm) of CdSO4. The growth of the plant was slightly enhanced with 1 ppm, while higher concentrations retarted growth and multiplication of fronds, the effect being concentration and dose dependant. Increase in malondialdehyde content was insignificant after the first week but a prolonged exposure led to significant (p < 0.05) increase of about 38% and 45% over the control in 20 and 30 ppm, respectively after four weeks. Catalase (EC 1.11.1.6; CAT) activity increased at low concentration, but it declined to 42% and 54% at 40 ppm after 6 and 30 days, respectively Superoxide dismutase (EC 1.15.1.1; SOD), ascorbate peroxidase (EC 1.11.1.11;APx) and glutathione reductase (EC 1.6.4.2) increased at both low as well at high concentrations, but a prolonged exposure to high concentration of Cd (40 ppm) led to significant (p < 0.05) decline in the mean activities of these antioxidant enzymes. Accumulation of Cd in biomass was concentration and time dependant However at high concentration of 40 ppm, Cd accumulation did not increase significantly (p < 0.05) with time. Increased activities of antioxidant enzymes in Cd treated plants suggest that metal tolerance in L. polyrrhiza might be associated to the changes of antioxidant enzymatic activities.