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Due to its own internal laws of development, Chinese medicine (CM) seems more inclined to empirical medicine in a relatively long historical period. It is considered to be lacking objective and unified clinical practice guidelines (CPGs), and the difficulties in diagnosis and therapeutic effect evaluation comes with it, have restricted its further inheritance, development and international communication. Over the years, our research group has been committed to improving the standardization theory and methodology of CM, also perfecting relative techniques for further application, which are all based on the stratified evidence scoring method. We have already applied this method to 45 issued guidelines, including 5 national guidelines, 3 industrial guidelines, and 37 formulation/revision social organization guidelines. The stratified evidence scoring method has been recognized and used widely. It helps scholars and applicators to study, formulate, publish and popularize the acupuncture therapy clinical practice guidelines better, thus further promotes the development of acupuncture therapy.
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BACKGROUND: IcarisideII (ICAII) could promote the differentiation of adipose tissue-derived stem cells (ADSCs) to Schwann cells (SCs), leading to improvement of erectile function (EF) and providing a realistic therapeutic option for the treatment of erectile dysfunction (ED). However, the underlying molecular mechanisms of ADSCs and ICAII in this process remain largely unclear. METHODS: ADSCs were treated with different concentrations of ICAII. Cell proliferation was determined by MTT assay. qRT-PCR and western blot were performed to detect expressions of SCs markers, signal transducer and activator of transcription-3 (STAT3), and microRNA-let-7i (let-7i). Luciferase reporter assay was conducted to verify the regulatory relationship between let-7i and STAT3. The detection of intracavernosal pressure (ICP) and the ratio of ICP/mean arterial pressure (MAP) were used to evaluate the EF in bilateral cavernous nerve injury (BCNI) rat models. RESULTS: ICAII promoted cell proliferation of ADSCs in a dose-dependent manner. The mRNA and protein levels of SCs markers were increased by ICAII treatment in a dose-dependent manner in ADSCs. Moreover, let-7i was significantly decreased in ICAII-treated ADSCs and upregulation of let-7i attenuated ICAII-induced promotion of SCs markers. In addition, STAT3 was a direct target of let-7i and upregulated in ICAII-treated ADSCs. Interestingly, overexpression of STAT3 abated the let-7i-mediated inhibition effect on differentiation of ADSCs to SCs and rescued the ICAII-mediated promotion effect on it. Besides, combination treatment of ADSCs and ICAII preserved the EF of BCNI rat models, which was undermined by let-7i overexpression. CONCLUSION: ICAII was effective for preserving EF by promoting the differentiation of ADSCs to SCs via modulating let-7i/STAT3 pathway.
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Animales , Masculino , Ratas , Células de Schwann/efectos de los fármacos , Flavonoides/farmacología , Diferenciación Celular/efectos de los fármacos , Tejido Adiposo/citología , Células Madre Mesenquimatosas/efectos de los fármacos , Disfunción Eréctil/tratamiento farmacológico , Transfección , Western Blotting , Ratas Sprague-Dawley , Modelos Animales de EnfermedadRESUMEN
Objective@#To investigate the histone acetylation level and histone deacetylase (HDAC) activity of peripheral blood CD4+ T lymphocytes in patients with oral lichen planus (OLP). @*Methods @#Twenty-three OLP patients were selected from August 2016 to January 2017 from the Stomatological Hospital, Southern Medical University. The diagnosis was confirmed by pathology, and the lesions were divided into a nonerosive OLP group (11 cases) and an erosive OLP group (12 cases). Ten healthy sex- and age-matched volunteers served as controls. Immunomagnetic beads were used to separate CD4+ T lymphocytes, and histones and nucleoproteins were extracted. The global histone H3/H4 acetylation levels and HDAC activity of CD4+ T lymphocytes from all subjects were detected by ELISA. The differences between the OLP and control groups were statistically analyzed. @*Results@#Global histone H3 hypoacetylation was observed in the OLP group compared with the control group (u = -2.410, P = 0.012). However, there was no significant difference in the serum CD4+ T lymphocyte histone H4 acetylation level between the OLP and control group (u = -1.412, P = 0.158). HDAC activity was significantly higher in the OLP group than in the healthy control group (F = 5.749, P = 0.023), and much higher HDAC activity was observed in the erosive group than in the nonerosive (P = 0.014) and healthy control groups (P = 0.001). The degree of histone H3 acetylation correlated negatively with increased HDAC activity in the OLP group (rs = -0.771, P < 0.001). There was no correlation between the level of histone H3 acetylation and HDAC activity in the healthy control group (rs = 0.382,P = 0.276). The histone H4 acetylation level in the OLP group showed no correlation with HDAC activity (rs = 0.149, P = 0.498), and the histone H4 acetylation level in the control group also showed no correlation with HDAC activity (rs = 0.527, P = 0.117).@*Conclusion @#Abnormal histone acetylation of CD4+ T lymphocytes in the peripheral blood of patients with OLP was identified and could be related to HDAC activity, suggesting that the epigenetic modification of histone acetylation may play a role in the pathogenesis of OLP.
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Objective @#To study the clinical periodontal status of patients with desquamative gingivitis (DG) and analyze the factors that influence clinical periodontal indicators.@*Methods @#A purposive sampling method was used to obtain 42 subjects for a DG case group and a control group. Periodontal clinical indicators were detected, and related factors were analyzed. @*Results @#The DG patients were primarily middle-aged women. Periodontal clinical indicators were more prevalent in individuals with oral lichen planus (OLP) and mucous membrane pemphigoid (MMP) than in the control group. Probing depth (PD) (χ2=53.058, P<0.001; χ2=32.989, P<0.001), clinical attachment (χ2=30.292, P<0.001; χ2=32.470, P<0.001) and the positive rate of bleeding on probing (BOP) (χ2=50.003, P<0.001; χ2=36.236, P<0.001) were higher in the OLP and MMP group than in the control group. The time interval between the onset and treatment of DG was correlated with PD (rs=0.523, P<0.001) and the rate of positive BOP sites (rs=0.377, P=0.014). @*Conclusion@# Patients with DG have obvious periodontal lesions. Early medical intervention is helpful for diagnosing and treating DG-related oral and systemic disease.
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ObjectiveTo constructe the quality-evaluation index system of clinical teaching of operating room. MethodsThe quality-evaluation indicator system was constructed using literature study and Delphi. Then the Precedence Chart and Analytic Hierarchy Process was adopted to ensure the index's weight. ResultsThe scale was formed by 5 items of level 1 and 21 items of level 2 of the index system. ConclusionThe index system has high reliability,which can be confirmed from experts' authority degree, their attentive involvement and the concentrating degree of the views. The Quality-Evaluation Index System ( QEIS ) is constructed which can be used to evaluate clinical teaching of operating room.
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<p><b>OBJECTIVE</b>To investigate whether cardiac tissue extracts from rats could mimic the cardiac microenvironment and act as a natural inducer in promoting the differentiation of bone marrow stromal cells (BMSCs) into cardiomyocytes.</p><p><b>METHODS</b>Three kinds of tissue extract or cell lysate [infarcted myocardial tissue extract (IMTE), normal myocardial tissue extract (NMTE) and cultured neonatal myocardial lysate (NML)] were employed to induce BMSCs into cardiomyocyte-like cells. The cells were harvested at each time point for reverse transcription-polymerase chain reaction (RT-PCR) detection, immunocytochemical analysis, and transmission electron microscopy.</p><p><b>RESULTS</b>After a 7-day induction, BMSCs were enlarged and polygonal in morphology. Myofilaments, striated sarcomeres, Z-lines, and more mitochondia were observed under transmission electron microscope. Elevated expression levels of cardiac-specific genes and proteins were also confirmed by RT-PCR and immunocytochemistry. Moreover, IMTE showed a greater capacity of differentiating BMSCs into cardiomyocyte-like cells.</p><p><b>CONCLUSIONS</b>Cardiac tissue extracts, especially IMTE, can effectively differentiate BMSCs into cardiomyocyte-like cells.</p>
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Animales , Masculino , Ratas , Secuencia de Bases , Células de la Médula Ósea , Biología Celular , Diferenciación Celular , Cartilla de ADN , Inmunohistoquímica , Microscopía Electrónica de Transmisión , Infarto del Miocardio , Metabolismo , Miocardio , Biología Celular , Ratas Sprague-Dawley , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Células del Estroma , Biología CelularRESUMEN
To assess the correlation and difference between various endoscopic grades and indices for assessment of endoscopic disease activity in inflammatory bowel disease [IBD]. Eighty consecutive patients with ulcerative colitis [UC], and 31 patients with Crohn's disease [CD] were evaluated based on multiple endoscopic criteria between June 2006 and February 2007 at the Department of Gastroenterology of Renji Hospital, Shanghai, China. Two experienced endoscopists evaluated the endoscopic findings using various systems independently. Data were analyzed using Kendall's coefficient of concordance and Spearman correlations. For the systems of UC and CD, Kendall's coefficients of concordance were 0.714 [p<0.001] and 0.342 [p<0.001]. There was no significant difference between the 2 systems of UC [all p<0.01]. However, no statistically significant concordances were found between Chinese Grading System of Crohn's Disease [CGSCD] and Crohn's Disease Endoscopic Index of Severity [CDEIS] [rs=0.323, p=0.076]. Significant differences in frequencies were detected among endoscopic systems for UC [p=0.001]. It was suggested in our study that endoscopic grading and scoring systems of UC had satisfactory concordance, and CGSCD showed room for improvement. However, the modified Baron scale was tend to severe category, while Jeroen classification was tend to mild category
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Humanos , Masculino , Femenino , Endoscopía , Colitis Ulcerosa , Enfermedad de CrohnRESUMEN
Prostaglandin E2, which is produced by cyclooxygenase (COX) during arachidonic acid metabolism, is considered to be related to colon carcinogenesis and selective COX-2 inhibitors may be effective for chemoprevention without the adverse side effects of non-selective, nonsteroid anti-inflammatory drugs. Therefore, the influence of JTE-522 (4-(4-cyclohexyl-2-methyloxazol-5-yl)-2-fluorobenzensulfonamide), a selective COX-2 inhibitor, was examined in azoxymethane(AOM)-induced rat colon carcinogenesis. A total of 40 male F344 rats were randomly divided into two groups. Group 1 received diet containing 0.015% JTE-522 and group 2 the normal diet without supplement as a control group; one week later, all rats were administered axozymethane (AOM) s.c. at a dose of 15 mg/kg body weight once a week for 3 successive weeks. At the termination of the experiment (30 weeks after the start), the multiplicity of colon cancer in group 1 was significantly less than that of group 2. The proliferating cell nuclear antigen (PCNA) indices for non-neoplastic cells of the colon mucosa in group 1 were also lower. These data thus suggest that JTE-522 has chemopreventive potential against colon carcinogenesis with decrease of mucosal cell proliferation in rats.
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Administración Oral , Alimentación Animal , Animales , Azoximetano/administración & dosificación , Bencenosulfonatos/farmacología , Carcinógenos/administración & dosificación , Transformación Celular Neoplásica , Neoplasias del Colon/fisiopatología , Inhibidores de la Ciclooxigenasa/farmacología , Mucosa Intestinal/citología , Masculino , Oxazoles/farmacología , Antígeno Nuclear de Célula en Proliferación/análisis , Distribución Aleatoria , Ratas , Ratas Endogámicas F344RESUMEN
Objective It has become more important to protect brain function in operation and post-operation period with the developmen of surgery therapy for congenital heart surgery.Methods The primary cultured neuron cell of l8-day-pregnan embryoid Sprague-Dawley rat's hippocampus has been selected as the experimental material.The experimental concentration of magnesium sulfate will be 0 mmol/L,1 mmol/L,2 mmol/L,4 mmol/L;0 mmol/L is for control group.Selecting c-fos protein as target for primary antibody.Results After immunohistochemistry procedure,we found the mortality of neuronal cultures with magnesium sulfate in different concentration was 89.47 %,49.35 %,52.56 %,90.67 %(P