RESUMEN
OBJECTIVE@#To screen the tumor markers of laryngeal carcinoma and to investigate their expression in preoperative and postoperative serum.@*METHOD@#The distinct protein in serum was detected in 32 cases of laryngeal carcinoma and 38 healthy people by IMAC-Cu proteinchip array and surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS). The distinct proteins in serum were detected in 32 cases of laryngeal carcinoma preoperation and within 10 days 15 cases of laryngeal carcinoma postoperation with the same methods. The discriminatory profiling between preoperative and postoperative patients was analyzed by Biomarker Wizard software and Biomarker Pattern software.@*RESULT@#The results showed that fifteen differentially expressed proteins in serum were screened by analysis of proteomic spectra of preoperative patients and normal subjects. Seventeen kinds of protein differentially expressed in serum were screened by analysis of proteomic spectra of preoperative and postoperative patients. Six kinds of protein (2958.52, 3796.89, 5148.86, 6115.57, 4052.18, and 7770.76) were obtained for making up patterns that was able to class the preoperative-team and postoperative-team. Corresponding correct ratio were 84.38% (27/32) and 73.33% (11/15).@*CONCLUSION@#The preliminary results suggest that classification system will provide a highly accurate and innovative approach for the early diagnosis of laryngeal carcinoma and judgement of prognosis. SELDI-TOF-MS technology is a useful tool for a high throughput screening of large-sized serum samples to discover potential biomarkers for laryngeal carcinoma.
Asunto(s)
Anciano , Femenino , Humanos , Masculino , Persona de Mediana Edad , Biomarcadores de Tumor , Sangre , Neoplasias Laríngeas , Sangre , Patología , Pronóstico , Análisis por Matrices de Proteínas , Métodos , Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción , MétodosRESUMEN
Objective:To screen the tumor markers of laryngeal carcinoma and to investigate their expression in preoperative and postoperative serum.Method:The distinct protein in serum was detected in 32 cases of laryngeal carcinoma and 38 healthy people by IMAC-Cu proteinchip array and surface-enhanced laser desorption/ionization time-of-flight mass spectrometry(SELDI-TOF-MS).The distinct proteins in serum were detected in 32 cases of laryngeal carcinoma preoperation and within 10 days 15 cases of laryngeal carcinoma postoperation with the same methods.The discriminatory profiling between preoperative and postoperative patients was analyzed by Biomarker Wizard software and Biomarker Pattern software.Result:The results showed that fifteen differentially expressed proteins in serum were screened by analysis of protemic spectra of preoprative patients and normal subjects.Seventeen kinds of protein differentially expressed in serum were screened by analysis of protemic spectra of preoperative and postoperative patients.Six kinds of protein(2 958.52,3 796.89,5 148.86,6 115.57, 052.18,and7 770.76)were obtained for making up patterns that was able to class the preoperative-team and postoperativeteam.Corresponding correct ratio were 84.38%(27/32)and 73.33%(11/15).Conclusion:The preliminary results suggest that classification system will provide a highly accurate and innovative approach for the arly dioagnosis of laryngeal carcinoma and judgement of prognosis.SELDI-TOF-MS technology is a useful tool for a high throughput screening of large-sized serum samples tO discover potential biomarkers for laryngeal carcinoma.
RESUMEN
Objective To observe the anatomic relation of the anterior inferior cerebellar artery and the vestibulocochlear nerve. Methods The anterior inferior cerebellar artery and branches related with the vestibulocochlear nerve in the cerebellopontine angle were examined with the operating microscopy in 20 adult head cadavers fixed with formalin. Results The incidence of the anterior inferior cerebellar artery, recurrent perforating artery and anterior inferior cerebellar artery loop was 100percent.The 80 percent recurrent perforating arteries passed between the facial nerve and the vestibulocochlear nerve. The 95 percent vestibulocochlear nerves had contact with the anterior inferior cerebellar artery or its branches. Among them, The 50 percent had two or more branches contacted with the vestibulocochlear nerves.Conclusion The result will supply useful anatomic data for the surgery in the cerebellopontine angle.