Identification of differentially expressed proteins in the liver of Oncomelania snails induced by Eomecon chinanthe sanguinarine / 中华预防医学杂志

<p><b>OBJECTIVE</b>To identify the differentially expressed proteins in the liver of Oncomelania snails induced by Eomecon chinanthe sanguinarine.</p><p><b>METHODS</b>Sanguinarine was extracted and purified from the dry powder of Eomecon chinanthe. Oncomelania snails were immersed in 5 mg/L sanguinarine (50 Oncomelania snails per 500 ml) or pure water for 36 h (25°C) and the livers were isolated from live snails. Total liver proteins were extracted and separated by two-dimensional gel electrophoresis. Electrophoretogram was analyzed by Image Master 2D 5.0 software. The differentially expressed proteins between sanguinarine group and pure water group were selected and analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and tandem mass spectrometry sequencing of tryptic peptides.</p><p><b>RESULTS</b>In terms of protein spots, 433 ± 14 and 385 ± 12 were observed in sanguinarine group and in water group respectively. The eleven identified differentially expressed proteins included tropomyosin, hypothetical protein XP_533132, actin 87E, keratin 6A, beta-tubulin, mitochondrial inner membrane protein isoform 4, keratin 2, allatostatin precursor, ENSANGP00000020184, actin-3 and ENSANGP00000013943. Among them, hypothetical protein XP_533132 and ENSANGP00000013943 were down-regulated and the other nine proteins were up-regulated in sanguinarine group.</p><p><b>CONCLUSION</b>Sanguinarine could alter the expression of proteins in livers of Oncomelania snails.</p>

Screening and identification of differentially expressed proteins between adult female and male worms of Schistosoma japonicum / 中华预防医学杂志

<p><b>OBJECTIVE</b>To screen and identify differentially expressed proteins between adult female and male worms of Schistosoma japonicum(S.japonicum).</p><p><b>METHODS</b>Two rabbits infected with the cercaria were perfused with saline in carotid, and approximately two hundred adult female and two hundred male worms of S.japonicum were collected. Approximately 300 microg soluble and hydrophobic proteins of adult female and male worms of S.japonicum were extracted and then the proteins were separated by two-dimensional gel electrophoresis respectively. The analysis using ImageMaster Platinum 2D 5.0 resulted in differentially expressed proteins between adult female and male worms, which were subjected to matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and tandem mass spectrometry sequencing of tryptic peptides.</p><p><b>RESULTS</b>There were (255 +/- 10) and (224 +/- 12) spots detected for soluble proteins and (200 +/- 11) and (132 +/- 8) spots for hydrophobic proteins from adult female and male worms respectively. Six differential proteins were identified, five up-regulated proteins in female worms were thioredoxin, putative ferritin-1 heavy chain, chain B in solution structure of the human ubiquitin-conjugating-enzyme-like protein Mms2-Ubiquitin Complex, heat shock protein 10, cytoplasmic fatty acid binding protein variant H; while only one up-regulated proteins in male worms was identified as 48 kDa histamine receptor subunit peptide 4.</p><p><b>CONCLUSION</b>Several differentially expressed proteins between female and male worms of S. japonicum were recognized through screening and identifying differential proteins between female and male worms of S.japonicum.</p>

Cloning and characterization of new genes of Schistosoma japonicum / 中南大学学报(医学版)

OBJECTIVE@#To clone and characterize new genes of Schistosoma japonicum, Sj, and to provide efficient vaccine candidates.@*METHODS@#Sj adult cDNA library was screened with rabbit sera raised against male worm soluble antigen. The inserted cDNA fragments from the positively selected clones were amplified with PCR and further sequenced, as well as characterized through internet NCBI GenBank software.@*RESULTS@#Eleven positive clones were obtained and two were verified by GenBank as new, including a novel gene designated as Sj-P8 (GenBank accession No. AF517843) and a new partial cDNA of Sj myosin (GenBank accession No. AY770506). The two new genes encoded a transmembrane protein of 75 amino acids and a myosin protein fragment of 212 amino acids respectively.@*CONCLUSION@#The newly obtained genes may provide useful information for the research on Sj vaccine.

Current Advances on Molecular Biology of Porcine Reproduc- tive and Respiratory Syndrome Virus / 微生物学通报

Porcine Reproductive and Respiratory Syndrome virus (PRRSV) is the etiological agent of Por- cine Reproductive and Respiratory Syndrome. We summarized the recent research progress on molecular bi- ology of PRRSV including the structure of genome, viral structural and Non-structural protein.