Effects of psoralen with long wave ultraviolet light on apoptosis and mitochondrial membrane potential in K562 cells / 中西医结合学报

To study the effects of psoralen (PSO) with long wave ultraviolet light (PUVA) on apoptosis and mitochondrial membrane potential in K562 cells.

Effects of psoralen plus long-wave ultraviolet-A on apoptosis of HL-60 leukemia cells / 中西医结合学报

OBJECTIVE: To explore the effects of psoralen (PSO) plus long-wave ultraviolet-A (PUVA) on apoptosis and expression of Fas ligand (FasL) in HL-60 leukemia cells. METHODS: The HL-60 cells were taken as the study objects and their apoptosis rates, ultrastructure changes and the expression of FasL were detected in order to observe the effects of PSO and ultraviolet-A (UVA) of wave length 360 nm. The factorial design and analysis of variance were used to analyze the interaction among the factors. RESULTS: PSO, UVA and PUVA all induced the apoptosis and the effects of PUVA were stronger than those of the other two. After HL-60 cells had been treated with PUVA, they all showed obvious ultrastructure changes due to apoptosis observed under the electron microscope. PSO, UVA and PUVA all decreased the expressions of FasL gene and protein. The effects of PUVA were stronger than those of the other two. CONCLUSIONS: PUVA can induce the apoptosis of HL-60 cells and the effects are stronger than those of PSO or UVA alone. The expression of FasL gene in HL-60 cells is down-regulated during the apoptosis induced by PUVA.

Effects of inactivated rabbit serum containing compound realgar and natural indigo tablet on cell line NB4 / 中西医结合学报

To explore the effects of inactivated rabbit serum containing compound realgar and natural indigo tablet (CRNIT) on cell line NB(4).

Effects of PUVA on Apoptosis and Expression of Fas in Leukemia Cells / 中国中医药信息杂志

Objective To explore the effects of psoralen (PSO) and long wave ultraviolet A (PUVA) on apoptosis and expression of Fas in HL-60,K562 and NB4 leukemia cells.Methods The cells were incubated with PSO in different concentrations irradiated with or without UVA.The changes of ultrastructure of cells were observed under the electron microscope.The expression of Fas gene was detected by fluorescent quantitation PCR.The apoptosis ratio and the expression of Fas protein were detected through the flow cytometry.The factorial design and analysis of variance were used to analyze the interaction among the factors.Results There were obvious ultrastructure changes about apoptosis in leukemia cells after treated with PUVA.PSO,UVA and PUVA all increased the apoptosis ratio and expression of Fas gene and protein,and the effects of PUVA were stronger than the other two (P

Growth-inhibiting effect of psoralen plus ultraviolet-A light therapy on K562 cells / 中西医结合学报

OBJECTIVE: To observe the effects of psoralen plus ultraviolet-A light (PUVA) on K562 cells and the relative mechanism. METHODS: The effects of psoralen, ultraviolet-A light and PUVA on K562 cells were assayed by monotetrazolium test (MTT). DNA content was analyzed by flow cytometry (FCM). The apoptotic rates of K562 cells treated with 40 and 80 microg/ml psoralen for 24 and 48 hours were assayed by Annexin-V-FITC/PI reagent kit on FCM respectively. The ultrastructures of apoptotic cells were observed by a transmission electron microscope (TEM). RESULTS: Either single psoralen therapy or single ultraviolet-A irradiation had inhibiting effect on K562 cells. The inhibiting effect of PUVA on K562 cells was stronger than that of the single psoralen therapy or single ultraviolet-A light irradiation (P<0.05). Apoptotic peak (AP) was detected by FCM. TEM test showed that K562 cells treated with PUVA were smaller, having condensed cell nucleus, assembled chromatin, disintegrated nucleus body and the majority of the cells appeared to be apoptotic conformation. CONCLUSION: Psoralen has inhibiting effect on K562 cells, and the effect of PUVA is more significant. It is suggested that 10 min irradiation and 40 microg/ml terminal concentration of psoralen be probably the best choice for PUVA. The inhibiting effect of PUVA is due to apoptosis.

Developing a finite element model of human head with true anatomic structure mandible / 生物医学工程学杂志

A finite element model of human mandible is developed from CT scan images by the technologies of three-dimensional reconstruction, image processing and meshing. The mandible model is connected to one modified head model of Hybrid III dummy with joint according to the anatomic structure and mechanical characteristics of the temporomandibular joint. Then a finite element model of the human head with the true anatomic structure mandible is developed. This model has been validated with the cadaver test results. It can be used in researches on the mechanism of craniofacial blunt-impact injury and on the assessment of injury severity.

The data management of 3D FE model reconstruction based on medical Images and the modeling for T10-T12 thoracic vertebrae / 生物医学工程学杂志

Setting up the 3D finite element (FE) model on the basis of medical images is a necessary method of biomechanical analysis. The pretreatment, including data processing of images and mesh generation, becomes the bottleneck of FE analysis, and this is especially the case of the modeling for thoracic vertebrae, because the shapes and data are complicated. In this paper, on the basis of the data for building a 3D FE model of thoracic vertebrae, we present some structures and types of data which are essential for the reconstruction of CT medical image, analyze some methods for obtaining and processing the data from images, and then advance a theory for data management based on mesh generation by block with the software MSC. Marc. A 3D-FE Model of T10-T12 thoracic vertebrae is set up, which keeps the configuration of the true one, and satisfies the requirements of biomechanical analysis. We focus affention on the data obtainment and management during medical image processing and FE modeling, all of which are very important for the biomechanical analysis. It is helpful and instructive to reconstruct some similar tissues of human body on the basis of the medical images from CT, MRI, etc.

Study on Realgar inducing apoptosis in T lymphocytic cell line CEM / 中西医结合学报

To study the Realgar induced T lymphocytic leukemia cell line CEM apoptosis in vitro.

Influence of arsenic bisulfide on the expression of HSP70 in K_(562)/ADM cell / 中国癌症杂志

Purpose:To study the expression of membrane protein HSP70 of K 562 /ADM cell using arsenic bisulfide(As 2S 2) treatment Methods:Membrane HSP70 and apoptosis of K 562 and multidrug resistant K 562 /ADM cell were studied by flow cytometry assay and RT PCR Results:Membrane HSP70 of K 562 and K 562 /ADM cell gradually increased from 12 to 24 hours with arsenic bisulfide treatment, and had higher expression at the level of HSP70mRNA Expression of membrane HSP70 and apoptosis of K 562 /ADM was higher than that of K 562 Conclusions:Arsenic bisulfide treatment can increase expression of membrane HSP70 of K 562 /ADM and K 562 cells.The change of membrane HSP70 is probably related to K 562 /ADM cell apoptosis

Effects of Psoralen and Ultraviolet Chemical Therapy on Cell Apoptosis and FasL Expression in Human Leukemia Cells / 中药新药与临床药理

Objective To explore the effects of psoralen(PSO)and long-wave ultraviolet chemical therapy(PUVA) on cell apoptosis and expressions of FasL in HL-60,K562,and NB4 leukemia cells.Methods The cells were taken as the studying objects and their apoptosis ratios,ultrastructure changes and the expressions of FasL were detected in or- der to observe the effects of PSO extracted from Chinese medicine and ultraviolet at 360 nm on human leukemia cells.The factor design and analysis of variance were used to analyze the interaction among the factors.Results(1)PSO,UVA and PUVA all induced the apoptosis and the effects of PUVA were stronger than the other two.(2)Obvious changes of ultra- structure of leukemia cells were found under electron microscope after treatment with PUVA.(3)PSO,UVA and PUVA down-regulated FasL gene and protein expression levels,and the effects of PUVA are the strongest.Conclusion PUVA can induce the apoptosis of human leukemia cells and its effects are the strongest.One of the pathway of PUVA to induce apoptosis is by down-regulating the expression of FasL gene.

INDUCTION OF APOPTOSIS BY COMPOUND "HUANG DAI" TABLET IN ACUTE PROMYELOCYTIC LEUKEMIA / 解放军医学杂志

To study the mechanism of therapeutie effect of compound "Huang Dai" tablet in acute promyelocytic leukemia, twenty one patients with acute promyelocytic leukemia were treated with compound "Huang Dai" tablet. Bone marrow cells of the patients were collected before treatment and after treatment, and membrane differentiating antigen (CD33 and CD11b), cell cycle and the apoptosis correlative protein(Fas and Apo 2.7 )were detected with flow cytometry. Results showed that the expression of CD33 was decreased and that of CD11b increased, G 2 /M phase of cell cycle was blocked, and the expressions of Fas and Apo 2.7 protein were increased significantly in all patents after treatment with compound "Huang Dai" tablet. These results suggested that the mechanism of therapeutic effect of compound "Huang Dai" tablet in acute promyelocytic leukemia was induction of both differentiation and apoptosis of leukemic cells, and cell apoptosis might be related to Fas and Apo2.7 protein.