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OBJECTIVE：To observe the p rotective effects of resveratrol （Res）on LPS-induced acute lung injury （ALI）model mice，and to explore its possible mechanism based on TLR 4/NF-κB pathway. METHODS：Kunming mice were divided into normal group，model group ，positive control group （dexamethasone，0.5 mg/kg），Res low-dose ，medium-dose and high-dose groups （50， 100，200 mg/kg），with 10 mice in each group. Normal group and model group were given normal saline intragastrically ，once a day，for 7 days；positive control group were intraperitoneally injected with Dexamethasone sodium phosphate injection ，once a day，for 3 days；Res groups were given relevant medicine intragastrically ，once a day ，for 7 days. After last administration ，all the mice except the normal group were dripped LPS （5 mg/kg）into the nose to induce ALI model. The apoptosis of neutrophils in BALF was observed by Hoechst 33242 staining；the apoptosis rate of neutrophils were detected by flow cytometry. The contents of IL- 6 and TNF-α in the plasma were detected by ELISA. After wet mass to dry mass （W/D）ratio of lung was E-mail：492234709@qq.com calculated，the morphological characteristics of lung tissue were observed by HE staining. Western blotting assay was used to detect the expression o f TLR 4 and NF-κ B in lung tissue. RESULTS ：In normal group ，there were few apoptotic neutrophils in the BALF ，and the lung tissue structure was intact ， without edema，hyperemia，exudation，inflammatory cell infiltration or other inflammatory manifestations. In model group ，the number of apoptotic neutrophils in BALF increased ，and the apoptotic rate of neutrophils were enhanced significantly （P＜0.01）； edema and hyperemia of lung tissue were significantly increased ，and the red consolidation area was observed ；the contents of IL- 6 and TNF-α in plasma，the ratio of lung W/D，the relative expression of TLR 4 and NF-κB in lung tissue were significantly increased （P＜0.01）. Compared with model group ，the number of apoptotic neutrophils in BALF were increased ，and the apoptotic rate of neutrophils were enhanced significantly （P＜0.05 or P＜0.01）above symptoms of lung tissue were improved to different extents ； the contents of IL- 6 and TNF-α in plasma，lung W/D ratio as well as relative expression of TLR 4 and NF-κB in lung tissue（except for Res low-dose group ）in administration groups were decreased significantly （P＜0.05 or P＜0.01）. CONCLUSIONS ：Res has a protective effect on ALI model mice ，the mechanism of which may be related to reducing the generation of inflammatory cytokines TNF-α and IL-6 by inhibiting TLR 4/NF-kB expression.
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Objective:To study the quality of life and postoperative complications in patients after laparoscopic cholecystectomy (LC).Methods:The data from 319 patients who were admitted to Nanjing First Hospital Affiliated to Nanjing Medical University and underwent LC from October 2013 to October 2017 were reviewed. These patients were assessed by a questionnaire which was based on the Gastrointestinal Quality of Life Index (GIQLI) before and after surgery.Results:The GIQLI scores on conscious symptoms, physiological function were significantly lower after surgery. The main postoperative complications were diarrhea (53.9%), decline in physical strength (30.1%), abdominal distension (25.4%), fatigue (26.9%) and abdominal pain (11.0%). Among patients with decline in physical strength, fatigue, and abdominal pain, 60.4%, 55.8%, and 51.4% of each of the groups, respectively, were associated with diarrhea. The incidences of severe diarrhea was 0.9%, severe fatigue was 0.3%, severe decline in physical strength 0% and severe abdominal pain 0%.Conclusions:The quality of life of patients declined after LC. The main postoperative complications were diarrhea, decline in physical strength, abdominal distension, fatigue and abdominal pain. However, the incidence of serious complications was small.
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Objective To analyze the incidence of choledocholithiasis in patients who underwent cholecystectomy in our hospital,and to determine the incidences of recurrent stones and choledocholithiasis combined with cholangitis after treatment for choledocholithiasis.Methods A retrospective study was conducted on 1 152 patients with common bile duct stones treated from January 2013 to July 2017 in our hospital.Results Of the 1152 patients with common bile duct stones,572 patients had a history of cholecystectomy,accounting for 49.65％ of all patients.402 patients had choledocholoithiasis combined with cholecystolithiasis (34.9％ of all patients),278 patients had choledocholithiasis without cholecystolithiasis (24.1％).Choledocholithiasis could occur after cholecystectomy.The time of onset of choledocholithiasis was 5 ～ 10 years after operation in 49.1％ of patients,and 200 of these patients with choledocholithiasis were treated with lithotripsy.There were 110 patients who underwent choledocholithotomy and cholecystectomy,and 90 patients who underwent ERCP and stone removal.There was no significant difference between the 2 groups (P ＞ 0.05).Choledocholithiasis complicated with cholangitis developed in 511 patients (44.3％ of all patients).These included 437 patients with acute mild cholangitis (85.5％) and acute obstructive suppurative cholangitis (14.5％).Recurrent choledocholithiasis combined with acute cholangitis after choledocholithotomy developed in 51 patients,and recurrent choledocholithiasis combined with cholangifts developed after ERCP in 50 patients.There was no significant difference between the two groups (P ＞0.05).Conclusions Choledocholithiasis after cholecystectomy accounted for a high proportion of patients.Recurrent choledocholithiasis could occur after choledocholithotomy.Common bile duct stones were susceptible to develop cholangitis.Thus,common bile duct stones should be treated as soon as possible.
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This paper describes the analysis of insitu polymerization of monomeric reactants (PMR) polyimide resin solution by reversed phase ion pair suppression chromatography. the mobile phase consists of acetonitrile and water. The methyl sulphonic acid was used as an ion-pair reagent in the mobile phase. The addition of sodium perchlorate was required to increase the ionic strength of the mobile phase. The information was obtained on monomeric isomer distribution of PMR polyimide resin solution and purity of prepared materials.