Studies on chemical constituent and quantitative determination of Mallotus apelta / 中国中药杂志

<p><b>OBJECTIVE</b>To investigate the chemical constituents and establish a quantitative method of Mallotus apelta.</p><p><b>METHOD</b>Compound was isolated by silica gel, Sephadex LH-20 column chromatography and Pre-HPLC chromatography. Its structure was identified by physicochemical properties and spectral evidences. The content of M. apelta was determined by HPLC. Chromatographic conditions included Inertsil ODS-3 C18 column (4.6 mm x 150 mm, 5 microm) and the mobile phase consisting of a mixture of methanol-water (24:76). The detection wavelength was set at 335 nm.</p><p><b>RESULT</b>One compound was isolated from n-butanol extract of the M. apelta and its structure was identified as vicenin II. The calibration cure was linear in the range of 0.053-10.60 microg (r = 0.9999), the average recovery was 99. 32%, RSD 1.82% (n = 6).</p><p><b>CONCLUSION</b>The compound was isolated from this plant for the first time. The method to determine the content of vicenin II by HPLC was established for the first time. This method is simple, accurate and reliable.</p>

Determination of verbascoside in herba of Galeobdolon Chinense by RP-HPLC / 中国中药杂志

<p><b>OBJECTIVE</b>To determine Verbascoside in Galeobdolon chinense.</p><p><b>METHOD</b>HPLC method was used, with the column packed with Kromasil C18(4.6 mm x 150 mm, 5 microns). The mobile phase was a mixture of acetonitrile and 1% acetic acid in a ratio of 13:87, and the ultraviolet wavelength was set at 350 nm.</p><p><b>RESULT</b>The average recovery was 97.0%, the RSD being 1.69%.</p><p><b>CONCLUSION</b>This method is sensitive and reliable. It can be used to determine being Verbascoside in Galeobdolon chinense.</p>

Studies on the chemical constituents in herba of Galeobdolon chinense (I) / 中国中药杂志

<p><b>OBJECTIVE</b>To study the chemical constituents in the Galeobdolon chinense.</p><p><b>METHOD</b>The chemical constituents were separated by means of chromatogrophy, and their structures were identified by spectra of FAB-MS, IR, UV, 1H-NMR, 13C-NMR, DEPT, HMQC and HMBC.</p><p><b>RESULT</b>Two compounds were obtained and identified as galeobdoside (I) whose structure was apigenin-7-O-beta-D-(6'-p-hydoxy-cinnamoyloxy)-mannoside and verbascoside (II).</p><p><b>CONCLUSION</b>Compound I was a new compound and compound II was obtained from this plant for the first time.</p>